Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.)
A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained whe...
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| Veröffentlicht in: | Plant cell reports 2006, Vol.24 (12), p.708-714 |
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| creator | Parasharami, V.A Naik, V.B Arnold, S. von Nadgauda, R.S Clapham, D.H |
| description | A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained when embryos were cultured for 6 days on 10 micromolar benzyl adenine-containing medium and then exposed to high osmoticum (0.5 M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for β-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta. |
| doi_str_mv | 10.1007/s00299-005-0019-z |
| format | Article |
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High levels of transient expression were obtained when embryos were cultured for 6 days on 10 micromolar benzyl adenine-containing medium and then exposed to high osmoticum (0.5 M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for β-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-005-0019-z</identifier><identifier>PMID: 16133348</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Base Sequence ; Biolistics ; cotyledons ; DNA Primers ; DNA, Plant ; embryo (plant) ; Embryos ; gene expression ; genetic transformation ; molecular sequence data ; nucleotide sequences ; particle bombardment ; Pine needles ; Pinus - embryology ; Pinus - genetics ; Pinus - physiology ; Pinus roxburghii ; plant genetics ; plant physiology ; plant regeneration ; Plants, Genetically Modified - embryology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - physiology ; Plasmids ; Polymerase Chain Reaction ; Seeds - growth & development ; Shoots ; Transformation, Genetic ; transgenes ; Transgenic plants ; Zea mays ; zygote</subject><ispartof>Plant cell reports, 2006, Vol.24 (12), p.708-714</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-c1f5fc6541a6ce84645b2ec548d04552a4f62fe6f1830efde7f1771bd335a30d3</citedby><cites>FETCH-LOGICAL-c381t-c1f5fc6541a6ce84645b2ec548d04552a4f62fe6f1830efde7f1771bd335a30d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16133348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parasharami, V.A</creatorcontrib><creatorcontrib>Naik, V.B</creatorcontrib><creatorcontrib>Arnold, S. von</creatorcontrib><creatorcontrib>Nadgauda, R.S</creatorcontrib><creatorcontrib>Clapham, D.H</creatorcontrib><title>Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained when embryos were cultured for 6 days on 10 micromolar benzyl adenine-containing medium and then exposed to high osmoticum (0.5 M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for β-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta.</description><subject>Base Sequence</subject><subject>Biolistics</subject><subject>cotyledons</subject><subject>DNA Primers</subject><subject>DNA, Plant</subject><subject>embryo (plant)</subject><subject>Embryos</subject><subject>gene expression</subject><subject>genetic transformation</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>particle bombardment</subject><subject>Pine needles</subject><subject>Pinus - embryology</subject><subject>Pinus - genetics</subject><subject>Pinus - physiology</subject><subject>Pinus roxburghii</subject><subject>plant genetics</subject><subject>plant physiology</subject><subject>plant regeneration</subject><subject>Plants, Genetically Modified - embryology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - physiology</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Seeds - growth & development</subject><subject>Shoots</subject><subject>Transformation, Genetic</subject><subject>transgenes</subject><subject>Transgenic plants</subject><subject>Zea mays</subject><subject>zygote</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcGKFDEQhoMo7uzqA3jR4EH00GsqSafTx2VxVVhQGBe8hXS60ptlujMm3eDM05txBgUvHioViu8vquon5AWwS2CseZ8Z421bMVaXgLbaPyIrkIJXnInvj8mKNRyqpgF5Rs5zfiiMFI16Ss5AgRBC6hXZr2fbbZDOyU7ZxzTaOcSJRk_Lb0lI97shzsFRHLu0i5naqacJB5ww_UF_i0upYNuNneZ8KLr7kOg2TEjffg3TkmmKP7tluA-Brm0aLt89I0-83WR8fsoX5O7mw7frT9Xtl4-fr69uKyc0zJUDX3unaglWOdRSybrj6GqpeybrmlvpFfeoPGjB0PfYeCg7d70QtRWsFxfkzbHvNsUfC-bZjCE73JRJMS7ZqEYxLcpF_gdCyxulVVvA1_-AD3FJU1nCaNZIzctbIDhCLsWcE3qzTWG0aWeAmYN95mifKfaZg31mXzQvT42XbsT-r-LkVwFeHQFvo7FDCtncrTkDwYCpciMhfgGuQaA8</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Parasharami, V.A</creator><creator>Naik, V.B</creator><creator>Arnold, S. von</creator><creator>Nadgauda, R.S</creator><creator>Clapham, D.H</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.)</title><author>Parasharami, V.A ; Naik, V.B ; Arnold, S. von ; Nadgauda, R.S ; Clapham, D.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-c1f5fc6541a6ce84645b2ec548d04552a4f62fe6f1830efde7f1771bd335a30d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Base Sequence</topic><topic>Biolistics</topic><topic>cotyledons</topic><topic>DNA Primers</topic><topic>DNA, Plant</topic><topic>embryo (plant)</topic><topic>Embryos</topic><topic>gene expression</topic><topic>genetic transformation</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>particle bombardment</topic><topic>Pine needles</topic><topic>Pinus - embryology</topic><topic>Pinus - genetics</topic><topic>Pinus - physiology</topic><topic>Pinus roxburghii</topic><topic>plant genetics</topic><topic>plant physiology</topic><topic>plant regeneration</topic><topic>Plants, Genetically Modified - embryology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - physiology</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Seeds - growth & development</topic><topic>Shoots</topic><topic>Transformation, Genetic</topic><topic>transgenes</topic><topic>Transgenic plants</topic><topic>Zea mays</topic><topic>zygote</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parasharami, V.A</creatorcontrib><creatorcontrib>Naik, V.B</creatorcontrib><creatorcontrib>Arnold, S. von</creatorcontrib><creatorcontrib>Nadgauda, R.S</creatorcontrib><creatorcontrib>Clapham, D.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parasharami, V.A</au><au>Naik, V.B</au><au>Arnold, S. von</au><au>Nadgauda, R.S</au><au>Clapham, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2006</date><risdate>2006</risdate><volume>24</volume><issue>12</issue><spage>708</spage><epage>714</epage><pages>708-714</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained when embryos were cultured for 6 days on 10 micromolar benzyl adenine-containing medium and then exposed to high osmoticum (0.5 M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for β-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>16133348</pmid><doi>10.1007/s00299-005-0019-z</doi><tpages>7</tpages></addata></record> |
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| ispartof | Plant cell reports, 2006, Vol.24 (12), p.708-714 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Base Sequence Biolistics cotyledons DNA Primers DNA, Plant embryo (plant) Embryos gene expression genetic transformation molecular sequence data nucleotide sequences particle bombardment Pine needles Pinus - embryology Pinus - genetics Pinus - physiology Pinus roxburghii plant genetics plant physiology plant regeneration Plants, Genetically Modified - embryology Plants, Genetically Modified - genetics Plants, Genetically Modified - physiology Plasmids Polymerase Chain Reaction Seeds - growth & development Shoots Transformation, Genetic transgenes Transgenic plants Zea mays zygote |
| title | Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine (Pinus roxbughii Sarg.) |
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