ICPBC and C12-ICPBC: two new red emitting, fluorescent Ca2+ indicators excited with visible light
Two new, visible-excited and red-emitting fluorescent Ca(2+) indicators were synthesized and the spectral profiles of their free and Ca(2+) bound forms were studied. The fluorescent properties of these probes are due to the extended conjugation of the chromeno[3',2':3,4]pyrido[1,2a][1,3]be...
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| Veröffentlicht in: | Cell calcium (Edinburgh) 2006-01, Vol.39 (1), p.3-11 |
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| container_title | Cell calcium (Edinburgh) |
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| creator | Roussakis, Emmanuel Liepouri, Fotini Nifli, Artemissia-Phoebe Castanas, Elias Deligeorgiev, Todor G Katerinopoulos, Haralambos E |
| description | Two new, visible-excited and red-emitting fluorescent Ca(2+) indicators were synthesized and the spectral profiles of their free and Ca(2+) bound forms were studied. The fluorescent properties of these probes are due to the extended conjugation of the chromeno[3',2':3,4]pyrido[1,2a][1,3]benzimidazole chromophore incorporated in their BAPTA-type, Ca(2+) chelating structure. The compounds, namely ICPBC and its N-dodecyl analog C12-ICPBC exhibit Ca(2+) dissociation constants of 7.7 and 18.0 microM, respectively. The fluorescence spectra of the probes showed a clear shift in excitation wavelength maxima upon Ca(2+) binding along with a large Stokes shift and changes in fluorescence intensity, indicating their potential use as Ca(2+) indicators. The ability of ICPBC to trace high calcium spikes was tested in the human HepG2 cell line with positive results. |
| doi_str_mv | 10.1016/j.ceca.2005.09.003 |
| format | Article |
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The ability of ICPBC to trace high calcium spikes was tested in the human HepG2 cell line with positive results.</description><subject>Algorithms</subject><subject>Benzimidazoles - chemical synthesis</subject><subject>Benzimidazoles - chemistry</subject><subject>Benzopyrans - chemical synthesis</subject><subject>Benzopyrans - chemistry</subject><subject>Calcium - analysis</subject><subject>Cell Line</subject><subject>Fluorescent Dyes - chemical synthesis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Indicators and Reagents - chemical synthesis</subject><subject>Indicators and Reagents - chemistry</subject><subject>Light</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Fluorescence</subject><issn>0143-4160</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAURT2AaCn8AQbkiQUSnu3ESdgg4qNSJRhgthz7tXWVJiV2KPx7UlqJ6elK5149HUIuGMQMmLxdxQaNjjlAGkMRA4gjMgaWiChhEkbk1PsVABQiYydkxCQXUqTZmOhp-fZQUt1YWjIe_aU7GrYtbXBLO7QU1y4E1yxu6Lzu2w69wSbQUvNr6hrrjA5t5yl-GxcGeuvCkn4576oaae0Wy3BGjue69nh-uBPy8fT4Xr5Es9fnaXk_iwwXLESJMDKpZFGhlBxkjlmGieBWVtrkgstkzgAsyoIVmakk6kTnXFfC2iK3WVKJCbna72669rNHH9TaDb_WtW6w7b2SWZrnIk0HkO9B07XedzhXm86tdfejGKidTLVSO5lqJ1NBoQaZQ-nysN5Xa7T_lYNJ8QvIxXGD</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>Roussakis, Emmanuel</creator><creator>Liepouri, Fotini</creator><creator>Nifli, Artemissia-Phoebe</creator><creator>Castanas, Elias</creator><creator>Deligeorgiev, Todor G</creator><creator>Katerinopoulos, Haralambos E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200601</creationdate><title>ICPBC and C12-ICPBC: two new red emitting, fluorescent Ca2+ indicators excited with visible light</title><author>Roussakis, Emmanuel ; Liepouri, Fotini ; Nifli, Artemissia-Phoebe ; Castanas, Elias ; Deligeorgiev, Todor G ; Katerinopoulos, Haralambos E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c231t-43c64b69be662068e77e432d6bac83264f100de69197cb6ea4a82ab3dd98d74b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Algorithms</topic><topic>Benzimidazoles - chemical synthesis</topic><topic>Benzimidazoles - chemistry</topic><topic>Benzopyrans - chemical synthesis</topic><topic>Benzopyrans - chemistry</topic><topic>Calcium - analysis</topic><topic>Cell Line</topic><topic>Fluorescent Dyes - chemical synthesis</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Humans</topic><topic>Indicators and Reagents - chemical synthesis</topic><topic>Indicators and Reagents - chemistry</topic><topic>Light</topic><topic>Microscopy, Confocal - methods</topic><topic>Microscopy, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roussakis, Emmanuel</creatorcontrib><creatorcontrib>Liepouri, Fotini</creatorcontrib><creatorcontrib>Nifli, Artemissia-Phoebe</creatorcontrib><creatorcontrib>Castanas, Elias</creatorcontrib><creatorcontrib>Deligeorgiev, Todor G</creatorcontrib><creatorcontrib>Katerinopoulos, Haralambos E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell calcium (Edinburgh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roussakis, Emmanuel</au><au>Liepouri, Fotini</au><au>Nifli, Artemissia-Phoebe</au><au>Castanas, Elias</au><au>Deligeorgiev, Todor G</au><au>Katerinopoulos, Haralambos E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ICPBC and C12-ICPBC: two new red emitting, fluorescent Ca2+ indicators excited with visible light</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>2006-01</date><risdate>2006</risdate><volume>39</volume><issue>1</issue><spage>3</spage><epage>11</epage><pages>3-11</pages><issn>0143-4160</issn><abstract>Two new, visible-excited and red-emitting fluorescent Ca(2+) indicators were synthesized and the spectral profiles of their free and Ca(2+) bound forms were studied. The fluorescent properties of these probes are due to the extended conjugation of the chromeno[3',2':3,4]pyrido[1,2a][1,3]benzimidazole chromophore incorporated in their BAPTA-type, Ca(2+) chelating structure. The compounds, namely ICPBC and its N-dodecyl analog C12-ICPBC exhibit Ca(2+) dissociation constants of 7.7 and 18.0 microM, respectively. The fluorescence spectra of the probes showed a clear shift in excitation wavelength maxima upon Ca(2+) binding along with a large Stokes shift and changes in fluorescence intensity, indicating their potential use as Ca(2+) indicators. The ability of ICPBC to trace high calcium spikes was tested in the human HepG2 cell line with positive results.</abstract><cop>Netherlands</cop><pmid>16236357</pmid><doi>10.1016/j.ceca.2005.09.003</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Algorithms Benzimidazoles - chemical synthesis Benzimidazoles - chemistry Benzopyrans - chemical synthesis Benzopyrans - chemistry Calcium - analysis Cell Line Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Humans Indicators and Reagents - chemical synthesis Indicators and Reagents - chemistry Light Microscopy, Confocal - methods Microscopy, Fluorescence |
| title | ICPBC and C12-ICPBC: two new red emitting, fluorescent Ca2+ indicators excited with visible light |
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