Identification and purification of a DNA-binding protein interacting with the promoter of 5′- nucleotidase in Dictyostelium discoideum

The developmental management of 5′- nucleotidase ( 5nt) expression in Dictyostelium discoideum has provided a focal point for studies of gene regulation at the level of transcription. To identify DNA–protein interactions involved in the 5nt regulation, EMSAs were performed using short oligonucleotid...

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