EUROPLUS™ ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis
The consensus on anti-neutrophil cytoplasmic antibody (ANCA) testing requires screening with indirect immunofluorescence (IIF) and confirmation in MPO- and PR3-ANCA specific assays. The EUROPLUS™ system combines in one incubation the conventional cell substrates with microdots of single antigens, i....
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| Veröffentlicht in: | Journal of immunological methods 2009-08, Vol.348 (1), p.67-73 |
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| creator | Damoiseaux, Jan Steller, Ulf Buschtez, Michael Vaessen, Mia Rosemann, Anke van Paassen, Pieter Stöcker, Winfried Fechner, Kai Cohen Tervaert, Jan Willem |
| description | The consensus on anti-neutrophil cytoplasmic antibody (ANCA) testing requires screening with indirect immunofluorescence (IIF) and confirmation in MPO- and PR3-ANCA specific assays. The EUROPLUS™ system combines in one incubation the conventional cell substrates with microdots of single antigens, i.e., MPO and PR3. We evaluated the diagnostic applicability of this new system for ANCA-associated vasculitis (AAV).
To assess the diagnostic performance of the EUROPLUS™ Granulocyte Mosaic, sera from 249 AAV patients, 85 disease controls and 27 healthy controls were analysed. Results were compared with a reference multi-testing algorithm based on IIF with ethanol-fixed granulocytes, direct and capture ELISAs for both MPO- and PR3-ANCA.
Based on the reference multi-testing algorithm 123 AAV patients were defined as having PR3-ANCA and 68 AAV patients as having MPO-ANCA (diagnostic sensitivity: 76.7%). For the EUROPLUS™ MPO and PR3 microdots the diagnostic sensitivity was 77.1% in the same AAV cohort. The concordance between both methods for PR3- and MPO-ANCA was 96.8% and 99.2%, respectively. In the control cohort the diagnostic specificity was 99.1% for the multi-testing algorithm and 98.2% for the EUROPLUS™ microdots.
The combination of conventional cell substrates and single MPO and PR3 antigen microdots greatly facilitates the identification of ANCA reactivity clinically relevant for AAV. Since our results obtained after a single incubation in the EUROPLUS™ system are highly concordant with the reference multi-testing algorithm (based on IIF, direct and capture ELISAs) the EUROPLUS™ system is advocated as an efficient test system. |
| doi_str_mv | 10.1016/j.jim.2009.07.001 |
| format | Article |
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To assess the diagnostic performance of the EUROPLUS™ Granulocyte Mosaic, sera from 249 AAV patients, 85 disease controls and 27 healthy controls were analysed. Results were compared with a reference multi-testing algorithm based on IIF with ethanol-fixed granulocytes, direct and capture ELISAs for both MPO- and PR3-ANCA.
Based on the reference multi-testing algorithm 123 AAV patients were defined as having PR3-ANCA and 68 AAV patients as having MPO-ANCA (diagnostic sensitivity: 76.7%). For the EUROPLUS™ MPO and PR3 microdots the diagnostic sensitivity was 77.1% in the same AAV cohort. The concordance between both methods for PR3- and MPO-ANCA was 96.8% and 99.2%, respectively. In the control cohort the diagnostic specificity was 99.1% for the multi-testing algorithm and 98.2% for the EUROPLUS™ microdots.
The combination of conventional cell substrates and single MPO and PR3 antigen microdots greatly facilitates the identification of ANCA reactivity clinically relevant for AAV. Since our results obtained after a single incubation in the EUROPLUS™ system are highly concordant with the reference multi-testing algorithm (based on IIF, direct and capture ELISAs) the EUROPLUS™ system is advocated as an efficient test system.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2009.07.001</identifier><identifier>PMID: 19615371</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adeno-associated virus ; Algorithms ; Anti-neutrophil cytoplasmic antibodies ; Antibodies, Antineutrophil Cytoplasmic - blood ; Autoantibodies ; Autoantibodies - blood ; Autoimmune Diseases - diagnosis ; Autoimmune Diseases - immunology ; Biological and medical sciences ; Cohort Studies ; Cross-Sectional Studies ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Granulocyte Colony-Stimulating Factor - analysis ; Granulomatosis with Polyangiitis - blood ; Granulomatosis with Polyangiitis - diagnosis ; Humans ; Immunoassay ; Interleukin-3 - analysis ; Molecular immunology ; Myeloblastin - analysis ; Protein Array Analysis ; Reagent Kits, Diagnostic ; Recombinant Fusion Proteins - analysis ; Recombinant Proteins ; Sensitivity and Specificity ; Techniques ; Vasculitis</subject><ispartof>Journal of immunological methods, 2009-08, Vol.348 (1), p.67-73</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-5e7e5e12947d4de76e31d7c7a3574259fd3c3d6941de3f3a19fbb213d9ca270c3</citedby><cites>FETCH-LOGICAL-c412t-5e7e5e12947d4de76e31d7c7a3574259fd3c3d6941de3f3a19fbb213d9ca270c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jim.2009.07.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21943392$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19615371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Damoiseaux, Jan</creatorcontrib><creatorcontrib>Steller, Ulf</creatorcontrib><creatorcontrib>Buschtez, Michael</creatorcontrib><creatorcontrib>Vaessen, Mia</creatorcontrib><creatorcontrib>Rosemann, Anke</creatorcontrib><creatorcontrib>van Paassen, Pieter</creatorcontrib><creatorcontrib>Stöcker, Winfried</creatorcontrib><creatorcontrib>Fechner, Kai</creatorcontrib><creatorcontrib>Cohen Tervaert, Jan Willem</creatorcontrib><title>EUROPLUS™ ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The consensus on anti-neutrophil cytoplasmic antibody (ANCA) testing requires screening with indirect immunofluorescence (IIF) and confirmation in MPO- and PR3-ANCA specific assays. The EUROPLUS™ system combines in one incubation the conventional cell substrates with microdots of single antigens, i.e., MPO and PR3. We evaluated the diagnostic applicability of this new system for ANCA-associated vasculitis (AAV).
To assess the diagnostic performance of the EUROPLUS™ Granulocyte Mosaic, sera from 249 AAV patients, 85 disease controls and 27 healthy controls were analysed. Results were compared with a reference multi-testing algorithm based on IIF with ethanol-fixed granulocytes, direct and capture ELISAs for both MPO- and PR3-ANCA.
Based on the reference multi-testing algorithm 123 AAV patients were defined as having PR3-ANCA and 68 AAV patients as having MPO-ANCA (diagnostic sensitivity: 76.7%). For the EUROPLUS™ MPO and PR3 microdots the diagnostic sensitivity was 77.1% in the same AAV cohort. The concordance between both methods for PR3- and MPO-ANCA was 96.8% and 99.2%, respectively. In the control cohort the diagnostic specificity was 99.1% for the multi-testing algorithm and 98.2% for the EUROPLUS™ microdots.
The combination of conventional cell substrates and single MPO and PR3 antigen microdots greatly facilitates the identification of ANCA reactivity clinically relevant for AAV. Since our results obtained after a single incubation in the EUROPLUS™ system are highly concordant with the reference multi-testing algorithm (based on IIF, direct and capture ELISAs) the EUROPLUS™ system is advocated as an efficient test system.</description><subject>Adeno-associated virus</subject><subject>Algorithms</subject><subject>Anti-neutrophil cytoplasmic antibodies</subject><subject>Antibodies, Antineutrophil Cytoplasmic - blood</subject><subject>Autoantibodies</subject><subject>Autoantibodies - blood</subject><subject>Autoimmune Diseases - diagnosis</subject><subject>Autoimmune Diseases - immunology</subject><subject>Biological and medical sciences</subject><subject>Cohort Studies</subject><subject>Cross-Sectional Studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Granulocyte Colony-Stimulating Factor - analysis</subject><subject>Granulomatosis with Polyangiitis - blood</subject><subject>Granulomatosis with Polyangiitis - diagnosis</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Interleukin-3 - analysis</subject><subject>Molecular immunology</subject><subject>Myeloblastin - analysis</subject><subject>Protein Array Analysis</subject><subject>Reagent Kits, Diagnostic</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Proteins</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>Vasculitis</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhS0EoqHwAGyQN7CbwT_jcQ2rELU0UiBRIWvLse8URzPj1nYidcWmT8Kj8SS4JIIdrM7mu-cenYPQS0pqSmj7dltv_VAzQlRNZE0IfYQm9EyySioiHqMJIYxVVAp1gp6ltCWFIC15ik6oaqngkk7Q9_P11XK1WH_5ef8DTz_PpvjDfDm7nK_wEJLx9h1eXXFsRoc_rZZFs7-GEQ_exuBCTtgPNzHsAedvgHuzCdHkEO-w8-Z6DCl7m3DofhtXJqVgvcng8N4ku-t99uk5etKZPsGLo56i9cX519lltVh-nM-mi8o2lOVKgAQBlKlGusaBbIFTJ600XMiGCdU5brlrVUMd8I4bqrrNhlHulDVMEstP0ZuDb4l7u4OU9eCThb43I4Rd0q0UZ62UzX9BRhhVgvIC0gNYqkgpQqdvoh9MvNOU6Id59FaXefTDPJpIXcovN6-O5rvNAO7vxXGPArw-AqUh03fRjNanP1x53XCuWOHeHzgone09RJ2sh9GC8xFs1i74f8T4BZ_QrN8</recordid><startdate>20090831</startdate><enddate>20090831</enddate><creator>Damoiseaux, Jan</creator><creator>Steller, Ulf</creator><creator>Buschtez, Michael</creator><creator>Vaessen, Mia</creator><creator>Rosemann, Anke</creator><creator>van Paassen, Pieter</creator><creator>Stöcker, Winfried</creator><creator>Fechner, Kai</creator><creator>Cohen Tervaert, Jan Willem</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090831</creationdate><title>EUROPLUS™ ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis</title><author>Damoiseaux, Jan ; Steller, Ulf ; Buschtez, Michael ; Vaessen, Mia ; Rosemann, Anke ; van Paassen, Pieter ; Stöcker, Winfried ; Fechner, Kai ; Cohen Tervaert, Jan Willem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-5e7e5e12947d4de76e31d7c7a3574259fd3c3d6941de3f3a19fbb213d9ca270c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adeno-associated virus</topic><topic>Algorithms</topic><topic>Anti-neutrophil cytoplasmic antibodies</topic><topic>Antibodies, Antineutrophil Cytoplasmic - blood</topic><topic>Autoantibodies</topic><topic>Autoantibodies - blood</topic><topic>Autoimmune Diseases - diagnosis</topic><topic>Autoimmune Diseases - immunology</topic><topic>Biological and medical sciences</topic><topic>Cohort Studies</topic><topic>Cross-Sectional Studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Granulocyte Colony-Stimulating Factor - analysis</topic><topic>Granulomatosis with Polyangiitis - blood</topic><topic>Granulomatosis with Polyangiitis - diagnosis</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Interleukin-3 - analysis</topic><topic>Molecular immunology</topic><topic>Myeloblastin - analysis</topic><topic>Protein Array Analysis</topic><topic>Reagent Kits, Diagnostic</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Recombinant Proteins</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Vasculitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Damoiseaux, Jan</creatorcontrib><creatorcontrib>Steller, Ulf</creatorcontrib><creatorcontrib>Buschtez, Michael</creatorcontrib><creatorcontrib>Vaessen, Mia</creatorcontrib><creatorcontrib>Rosemann, Anke</creatorcontrib><creatorcontrib>van Paassen, Pieter</creatorcontrib><creatorcontrib>Stöcker, Winfried</creatorcontrib><creatorcontrib>Fechner, Kai</creatorcontrib><creatorcontrib>Cohen Tervaert, Jan Willem</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Damoiseaux, Jan</au><au>Steller, Ulf</au><au>Buschtez, Michael</au><au>Vaessen, Mia</au><au>Rosemann, Anke</au><au>van Paassen, Pieter</au><au>Stöcker, Winfried</au><au>Fechner, Kai</au><au>Cohen Tervaert, Jan Willem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EUROPLUS™ ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2009-08-31</date><risdate>2009</risdate><volume>348</volume><issue>1</issue><spage>67</spage><epage>73</epage><pages>67-73</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The consensus on anti-neutrophil cytoplasmic antibody (ANCA) testing requires screening with indirect immunofluorescence (IIF) and confirmation in MPO- and PR3-ANCA specific assays. The EUROPLUS™ system combines in one incubation the conventional cell substrates with microdots of single antigens, i.e., MPO and PR3. We evaluated the diagnostic applicability of this new system for ANCA-associated vasculitis (AAV).
To assess the diagnostic performance of the EUROPLUS™ Granulocyte Mosaic, sera from 249 AAV patients, 85 disease controls and 27 healthy controls were analysed. Results were compared with a reference multi-testing algorithm based on IIF with ethanol-fixed granulocytes, direct and capture ELISAs for both MPO- and PR3-ANCA.
Based on the reference multi-testing algorithm 123 AAV patients were defined as having PR3-ANCA and 68 AAV patients as having MPO-ANCA (diagnostic sensitivity: 76.7%). For the EUROPLUS™ MPO and PR3 microdots the diagnostic sensitivity was 77.1% in the same AAV cohort. The concordance between both methods for PR3- and MPO-ANCA was 96.8% and 99.2%, respectively. In the control cohort the diagnostic specificity was 99.1% for the multi-testing algorithm and 98.2% for the EUROPLUS™ microdots.
The combination of conventional cell substrates and single MPO and PR3 antigen microdots greatly facilitates the identification of ANCA reactivity clinically relevant for AAV. Since our results obtained after a single incubation in the EUROPLUS™ system are highly concordant with the reference multi-testing algorithm (based on IIF, direct and capture ELISAs) the EUROPLUS™ system is advocated as an efficient test system.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19615371</pmid><doi>10.1016/j.jim.2009.07.001</doi><tpages>7</tpages></addata></record> |
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| subjects | Adeno-associated virus Algorithms Anti-neutrophil cytoplasmic antibodies Antibodies, Antineutrophil Cytoplasmic - blood Autoantibodies Autoantibodies - blood Autoimmune Diseases - diagnosis Autoimmune Diseases - immunology Biological and medical sciences Cohort Studies Cross-Sectional Studies Fundamental and applied biological sciences. Psychology Fundamental immunology Granulocyte Colony-Stimulating Factor - analysis Granulomatosis with Polyangiitis - blood Granulomatosis with Polyangiitis - diagnosis Humans Immunoassay Interleukin-3 - analysis Molecular immunology Myeloblastin - analysis Protein Array Analysis Reagent Kits, Diagnostic Recombinant Fusion Proteins - analysis Recombinant Proteins Sensitivity and Specificity Techniques Vasculitis |
| title | EUROPLUS™ ANCA BIOCHIP mosaic: PR3 and MPO antigen microdots improve the laboratory diagnostics of ANCA-associated vasculitis |
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