Determination of ibuprofen and tetrazepam in human urine by micellar electrokinetic capillary chromatography
A new micellar electrokinetic capillary chromatography method (MEKC) is proposed for the determination of ibuprofen and tetrazepam in human urine samples over a concentration range of therapeutic interest. A fused silica capillary (60 cm x 75 μm) is used. Ibuprofen and tetrazepam are detected via UV...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2006, Vol.384 (1), p.208-214 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Nevado, J. J. Berzas Flores, J. Rodríguez Peñalvo, G. Castañeda Dorado, R. M. Rodríguez |
| description | A new micellar electrokinetic capillary chromatography method (MEKC) is proposed for the determination of ibuprofen and tetrazepam in human urine samples over a concentration range of therapeutic interest. A fused silica capillary (60 cm x 75 μm) is used. Ibuprofen and tetrazepam are detected via UV detection at 220 and 228 nm, respectively. Separation is performed at 25 °C and at a separation voltage of 30 kV, with 15 mM borate buffer (pH 10.2) containing 40 mM sodium dodecylsulfate as the electrolyte solution. Under these conditions the analytes were separated in |
| doi_str_mv | 10.1007/s00216-005-0128-0 |
| format | Article |
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Prior to determination, the samples are purified and enriched by means of an extraction-preconcentration step with a preconditioned C₁₈ cartridge and by eluting the compounds with methanol. Good linearity, accuracy, precision, robustness and solution stability were achieved for the technique. Detection limits of 200 μg L-¹ for ibuprofen and 300 μg L-¹ for tetrazepam were obtained. 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J. Berzas</creatorcontrib><creatorcontrib>Flores, J. Rodríguez</creatorcontrib><creatorcontrib>Peñalvo, G. Castañeda</creatorcontrib><creatorcontrib>Dorado, R. M. Rodríguez</creatorcontrib><title>Determination of ibuprofen and tetrazepam in human urine by micellar electrokinetic capillary chromatography</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><description>A new micellar electrokinetic capillary chromatography method (MEKC) is proposed for the determination of ibuprofen and tetrazepam in human urine samples over a concentration range of therapeutic interest. A fused silica capillary (60 cm x 75 μm) is used. Ibuprofen and tetrazepam are detected via UV detection at 220 and 228 nm, respectively. Separation is performed at 25 °C and at a separation voltage of 30 kV, with 15 mM borate buffer (pH 10.2) containing 40 mM sodium dodecylsulfate as the electrolyte solution. Under these conditions the analytes were separated in <11 min. Sulfamethazine is used as an internal standard. Prior to determination, the samples are purified and enriched by means of an extraction-preconcentration step with a preconditioned C₁₈ cartridge and by eluting the compounds with methanol. Good linearity, accuracy, precision, robustness and solution stability were achieved for the technique. Detection limits of 200 μg L-¹ for ibuprofen and 300 μg L-¹ for tetrazepam were obtained. These analytes were then determined in real urine using the technique.</description><subject>Benzodiazepines - chemistry</subject><subject>Benzodiazepines - urine</subject><subject>capillary electrophoresis</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Humans</subject><subject>ibuprofen</subject><subject>Ibuprofen - chemistry</subject><subject>Ibuprofen - urine</subject><subject>Robustness</subject><subject>Sensitivity and Specificity</subject><subject>Solutions</subject><subject>Temperature</subject><subject>Tetrazepam</subject><subject>Time Factors</subject><subject>urine</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0Eoh_wA7iAT9xSZhx_JMeqQFupEgfK2bKzk67bJA62c1h-Pdnuih45zYeeeTUzL2MfEC4QwHzJAAJ1BaAqQNFU8IqdosamElrB63-5FCfsLOdHAFQN6rfsBHUtGqHEKRu-UqE0hsmVECceex78MqfY08TdtOGFSnJ_aHYjDxPfLqOb-JLCRNzv-Bg6GgaXOA3UlRSf1n4JHe_cHPb9He-2KY6uxIfk5u3uHXvTuyHT-2M8Z_ffv91f3VR3P65vry7vqq5WslStqbVvySEZQbLVngANSCJvhOqBBMKmluix7RvnDLW4WQsjO628R1mfs88H2fWO3wvlYseQnzedKC7ZaqMaDRr-C4pWgZSmXUE8gF2KOSfq7ZzCuB5oEezeCnuwwq5W2L0Vdi_-8Si--JE2LxPH36_ApwPQu2jdQwrZ_vopAGuARrVS6vovGSmPSQ</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Nevado, J. 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Rodríguez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-9736b9ea1e72e496be01704eeb725f0e210d341b19f8aa7e91d1b174c65bb143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Benzodiazepines - chemistry</topic><topic>Benzodiazepines - urine</topic><topic>capillary electrophoresis</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Humans</topic><topic>ibuprofen</topic><topic>Ibuprofen - chemistry</topic><topic>Ibuprofen - urine</topic><topic>Robustness</topic><topic>Sensitivity and Specificity</topic><topic>Solutions</topic><topic>Temperature</topic><topic>Tetrazepam</topic><topic>Time Factors</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nevado, J. J. Berzas</creatorcontrib><creatorcontrib>Flores, J. Rodríguez</creatorcontrib><creatorcontrib>Peñalvo, G. 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Separation is performed at 25 °C and at a separation voltage of 30 kV, with 15 mM borate buffer (pH 10.2) containing 40 mM sodium dodecylsulfate as the electrolyte solution. Under these conditions the analytes were separated in <11 min. Sulfamethazine is used as an internal standard. Prior to determination, the samples are purified and enriched by means of an extraction-preconcentration step with a preconditioned C₁₈ cartridge and by eluting the compounds with methanol. Good linearity, accuracy, precision, robustness and solution stability were achieved for the technique. Detection limits of 200 μg L-¹ for ibuprofen and 300 μg L-¹ for tetrazepam were obtained. These analytes were then determined in real urine using the technique.</abstract><cop>Germany</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>16328252</pmid><doi>10.1007/s00216-005-0128-0</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Springer Online Journals Complete |
| subjects | Benzodiazepines - chemistry Benzodiazepines - urine capillary electrophoresis Chromatography, Micellar Electrokinetic Capillary - methods Humans ibuprofen Ibuprofen - chemistry Ibuprofen - urine Robustness Sensitivity and Specificity Solutions Temperature Tetrazepam Time Factors urine |
| title | Determination of ibuprofen and tetrazepam in human urine by micellar electrokinetic capillary chromatography |
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