Determination of ibuprofen and tetrazepam in human urine by micellar electrokinetic capillary chromatography

A new micellar electrokinetic capillary chromatography method (MEKC) is proposed for the determination of ibuprofen and tetrazepam in human urine samples over a concentration range of therapeutic interest. A fused silica capillary (60 cm x 75 μm) is used. Ibuprofen and tetrazepam are detected via UV...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2006, Vol.384 (1), p.208-214
Hauptverfasser:	Nevado, J. J. Berzas, Flores, J. Rodríguez, Peñalvo, G. Castañeda, Dorado, R. M. Rodríguez
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzodiazepines - chemistry Benzodiazepines - urine capillary electrophoresis Chromatography, Micellar Electrokinetic Capillary - methods Humans ibuprofen Ibuprofen - chemistry Ibuprofen - urine Robustness Sensitivity and Specificity Solutions Temperature Tetrazepam Time Factors urine
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Zusammenfassung:	A new micellar electrokinetic capillary chromatography method (MEKC) is proposed for the determination of ibuprofen and tetrazepam in human urine samples over a concentration range of therapeutic interest. A fused silica capillary (60 cm x 75 μm) is used. Ibuprofen and tetrazepam are detected via UV detection at 220 and 228 nm, respectively. Separation is performed at 25 °C and at a separation voltage of 30 kV, with 15 mM borate buffer (pH 10.2) containing 40 mM sodium dodecylsulfate as the electrolyte solution. Under these conditions the analytes were separated in
ISSN:	1618-2642 1618-2650
DOI:	10.1007/s00216-005-0128-0