Differential in vivo response of soft-shell clam hemocytes against two strains of Vibrio splendidus: Changes in cell structure, numbers and adherence
Host–pathogen interaction models in aquatic species are useful tools for understanding the pathogenicity of diseases in cultured and wild populations. In this study we report the differential in vivo response of soft-shell clam ( Mya arenaria) hemocytes against two strains of Vibrio splendidus. Resp...
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| Veröffentlicht in: | Journal of invertebrate pathology 2009-09, Vol.102 (1), p.50-56 |
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| creator | Mateo, Dante R. Siah, Ahmed Araya, Mebrahtu T. Berthe, Franck C.J. Johnson, Gerry R. Greenwood, Spencer J. |
| description | Host–pathogen interaction models in aquatic species are useful tools for understanding the pathogenicity of diseases in cultured and wild populations. In this study we report the differential
in vivo response of soft-shell clam (
Mya arenaria) hemocytes against two strains of
Vibrio splendidus. Responses were measured 24
h after injecting into the posterior adductor muscle either an endemic wild-type strain (7SHRW) or a strain associated with oyster mortalities (LGP32-GFP). Changes in hemocyte structure (percentage of rounded cells) were assessed microscopically. Changes in adherence and hemocyte numbers were analyzed by flow-cytometric cell counting. Increased percentages of rounded cells were found in response to both strains. However, values from the group infected with LGP32-GFP were significantly higher (
p
<
0.01) than with 7SHRW. The cell adherence was markedly diminished (
p
<
0.001) by LGP32-GFP whereas 7SHRW did not change it significantly. Increased numbers of hemocytes (
p
<
0.001) were induced by LGP32-GFP, while no significant changes were found after infection with 7SHRW. These results show the regulatory capacity of soft-shell clams hemocytes to perform specific responses against different strains of
V. splendidus. |
| doi_str_mv | 10.1016/j.jip.2009.06.008 |
| format | Article |
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in vivo response of soft-shell clam (
Mya arenaria) hemocytes against two strains of
Vibrio splendidus. Responses were measured 24
h after injecting into the posterior adductor muscle either an endemic wild-type strain (7SHRW) or a strain associated with oyster mortalities (LGP32-GFP). Changes in hemocyte structure (percentage of rounded cells) were assessed microscopically. Changes in adherence and hemocyte numbers were analyzed by flow-cytometric cell counting. Increased percentages of rounded cells were found in response to both strains. However, values from the group infected with LGP32-GFP were significantly higher (
p
<
0.01) than with 7SHRW. The cell adherence was markedly diminished (
p
<
0.001) by LGP32-GFP whereas 7SHRW did not change it significantly. Increased numbers of hemocytes (
p
<
0.001) were induced by LGP32-GFP, while no significant changes were found after infection with 7SHRW. These results show the regulatory capacity of soft-shell clams hemocytes to perform specific responses against different strains of
V. splendidus.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2009.06.008</identifier><identifier>PMID: 19576219</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animal aquaculture ; Animal productions ; Animals ; Biological and medical sciences ; Cell Adhesion ; Fundamental and applied biological sciences. Psychology ; Hemocytes ; Hemocytes - immunology ; Hemocytes - parasitology ; Hemocytes - pathology ; Host-Parasite Interactions - physiology ; Host-response ; Invertebrate aquaculture ; Invertebrates ; Marine ; Mollusca ; Mya - immunology ; Mya - parasitology ; Mya arenaria ; Pathology ; Phenotype ; Vibrio ; Vibrio Infections - immunology ; Vibrio Infections - pathology ; Vibrio splendidus</subject><ispartof>Journal of invertebrate pathology, 2009-09, Vol.102 (1), p.50-56</ispartof><rights>2009 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8a5007b5b81e1612fb9b877edfb55e02a6a2bf2df4f580f670c24bc0770991543</citedby><cites>FETCH-LOGICAL-c412t-8a5007b5b81e1612fb9b877edfb55e02a6a2bf2df4f580f670c24bc0770991543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jip.2009.06.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21859540$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19576219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mateo, Dante R.</creatorcontrib><creatorcontrib>Siah, Ahmed</creatorcontrib><creatorcontrib>Araya, Mebrahtu T.</creatorcontrib><creatorcontrib>Berthe, Franck C.J.</creatorcontrib><creatorcontrib>Johnson, Gerry R.</creatorcontrib><creatorcontrib>Greenwood, Spencer J.</creatorcontrib><title>Differential in vivo response of soft-shell clam hemocytes against two strains of Vibrio splendidus: Changes in cell structure, numbers and adherence</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>Host–pathogen interaction models in aquatic species are useful tools for understanding the pathogenicity of diseases in cultured and wild populations. In this study we report the differential
in vivo response of soft-shell clam (
Mya arenaria) hemocytes against two strains of
Vibrio splendidus. Responses were measured 24
h after injecting into the posterior adductor muscle either an endemic wild-type strain (7SHRW) or a strain associated with oyster mortalities (LGP32-GFP). Changes in hemocyte structure (percentage of rounded cells) were assessed microscopically. Changes in adherence and hemocyte numbers were analyzed by flow-cytometric cell counting. Increased percentages of rounded cells were found in response to both strains. However, values from the group infected with LGP32-GFP were significantly higher (
p
<
0.01) than with 7SHRW. The cell adherence was markedly diminished (
p
<
0.001) by LGP32-GFP whereas 7SHRW did not change it significantly. Increased numbers of hemocytes (
p
<
0.001) were induced by LGP32-GFP, while no significant changes were found after infection with 7SHRW. These results show the regulatory capacity of soft-shell clams hemocytes to perform specific responses against different strains of
V. splendidus.</description><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemocytes</subject><subject>Hemocytes - immunology</subject><subject>Hemocytes - parasitology</subject><subject>Hemocytes - pathology</subject><subject>Host-Parasite Interactions - physiology</subject><subject>Host-response</subject><subject>Invertebrate aquaculture</subject><subject>Invertebrates</subject><subject>Marine</subject><subject>Mollusca</subject><subject>Mya - immunology</subject><subject>Mya - parasitology</subject><subject>Mya arenaria</subject><subject>Pathology</subject><subject>Phenotype</subject><subject>Vibrio</subject><subject>Vibrio Infections - immunology</subject><subject>Vibrio Infections - pathology</subject><subject>Vibrio splendidus</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhCMEYoeFB-CCfIETGdqe2InhhIZfaSUuwNWynfaOR4kTbGfQPgjvi6MZwQ1O7ba-KpW6quophS0FKl4dt0c_bxmA3ILYAnT3qg0FKWrogN-vNgCM1QwovaoepXSE8uJCPqyuqOStYFRuql_vvHMYMWSvB-IDOfnTRCKmeQoJyeRImlyu0wGHgdhBj-SA42TvMiaib7UPKZP8cyIpx3VZBd-9ib78zAOG3vdLek32Bx1ui6L429Wo0IvNS8SXJCyjwVjMQk90f1ijWHxcPXB6SPjkMq-rbx_ef91_qm--fPy8f3tT24ayXHeaA7SGm44iFZQ5I03Xttg7wzkC00Iz41jvGsc7cKIFyxpjoW1BSsqb3XX14uw7x-nHgimr0ac1oQ44LUmJlnesE7v_ggxaIeWOFpCeQRunlCI6NUc_6ninKKi1NHVUpTS1lqZAqFJa0Ty7mC9mxP6v4tJSAZ5fAJ2sHlzUwfr0h2O045I3ULg3Zw7LzU4eo0rWr_fsfUSbVT_5f8T4Df9wtus</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Mateo, Dante R.</creator><creator>Siah, Ahmed</creator><creator>Araya, Mebrahtu T.</creator><creator>Berthe, Franck C.J.</creator><creator>Johnson, Gerry R.</creator><creator>Greenwood, Spencer J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Differential in vivo response of soft-shell clam hemocytes against two strains of Vibrio splendidus: Changes in cell structure, numbers and adherence</title><author>Mateo, Dante R. ; Siah, Ahmed ; Araya, Mebrahtu T. ; Berthe, Franck C.J. ; Johnson, Gerry R. ; Greenwood, Spencer J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8a5007b5b81e1612fb9b877edfb55e02a6a2bf2df4f580f670c24bc0770991543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemocytes</topic><topic>Hemocytes - immunology</topic><topic>Hemocytes - parasitology</topic><topic>Hemocytes - pathology</topic><topic>Host-Parasite Interactions - physiology</topic><topic>Host-response</topic><topic>Invertebrate aquaculture</topic><topic>Invertebrates</topic><topic>Marine</topic><topic>Mollusca</topic><topic>Mya - immunology</topic><topic>Mya - parasitology</topic><topic>Mya arenaria</topic><topic>Pathology</topic><topic>Phenotype</topic><topic>Vibrio</topic><topic>Vibrio Infections - immunology</topic><topic>Vibrio Infections - pathology</topic><topic>Vibrio splendidus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mateo, Dante R.</creatorcontrib><creatorcontrib>Siah, Ahmed</creatorcontrib><creatorcontrib>Araya, Mebrahtu T.</creatorcontrib><creatorcontrib>Berthe, Franck C.J.</creatorcontrib><creatorcontrib>Johnson, Gerry R.</creatorcontrib><creatorcontrib>Greenwood, Spencer J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mateo, Dante R.</au><au>Siah, Ahmed</au><au>Araya, Mebrahtu T.</au><au>Berthe, Franck C.J.</au><au>Johnson, Gerry R.</au><au>Greenwood, Spencer J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential in vivo response of soft-shell clam hemocytes against two strains of Vibrio splendidus: Changes in cell structure, numbers and adherence</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>102</volume><issue>1</issue><spage>50</spage><epage>56</epage><pages>50-56</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>Host–pathogen interaction models in aquatic species are useful tools for understanding the pathogenicity of diseases in cultured and wild populations. In this study we report the differential
in vivo response of soft-shell clam (
Mya arenaria) hemocytes against two strains of
Vibrio splendidus. Responses were measured 24
h after injecting into the posterior adductor muscle either an endemic wild-type strain (7SHRW) or a strain associated with oyster mortalities (LGP32-GFP). Changes in hemocyte structure (percentage of rounded cells) were assessed microscopically. Changes in adherence and hemocyte numbers were analyzed by flow-cytometric cell counting. Increased percentages of rounded cells were found in response to both strains. However, values from the group infected with LGP32-GFP were significantly higher (
p
<
0.01) than with 7SHRW. The cell adherence was markedly diminished (
p
<
0.001) by LGP32-GFP whereas 7SHRW did not change it significantly. Increased numbers of hemocytes (
p
<
0.001) were induced by LGP32-GFP, while no significant changes were found after infection with 7SHRW. These results show the regulatory capacity of soft-shell clams hemocytes to perform specific responses against different strains of
V. splendidus.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>19576219</pmid><doi>10.1016/j.jip.2009.06.008</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2011 |
| ispartof | Journal of invertebrate pathology, 2009-09, Vol.102 (1), p.50-56 |
| issn | 0022-2011 1096-0805 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67582863 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animal aquaculture Animal productions Animals Biological and medical sciences Cell Adhesion Fundamental and applied biological sciences. Psychology Hemocytes Hemocytes - immunology Hemocytes - parasitology Hemocytes - pathology Host-Parasite Interactions - physiology Host-response Invertebrate aquaculture Invertebrates Marine Mollusca Mya - immunology Mya - parasitology Mya arenaria Pathology Phenotype Vibrio Vibrio Infections - immunology Vibrio Infections - pathology Vibrio splendidus |
| title | Differential in vivo response of soft-shell clam hemocytes against two strains of Vibrio splendidus: Changes in cell structure, numbers and adherence |
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