Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4 ,6 -disulfate) and glucuronate(3 -sulfate) residues
Unique disaccharide composition of novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. 2S, 3S, 4S, and 6S represent 2-sulfate, 3-sulfate, 4-sulfate and 6-sulfate, respectively. Novel chondroitin sulfate (CS) chains w...
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| Veröffentlicht in: | Carbohydrate research 2009-08, Vol.344 (12), p.1526-1532 |
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| creator | Shetty, Ajaya Kumar Kobayashi, Takanari Mizumoto, Shuji Narumi, Masaki Kudo, Yoshiaki Yamada, Shuhei Sugahara, Kazuyuki |
| description | Unique disaccharide composition of novel chondroitin sulfate from squid liver integument rich in
N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. 2S, 3S, 4S, and 6S represent 2-sulfate, 3-sulfate, 4-sulfate and 6-sulfate, respectively.
Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6
kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1→3GalNAc(6-sulfate), GlcAβ1→3GalNAc(4-sulfate), and GlcAβ1→3GalNAc(4,6-disulfate) as the major ones, significant amounts of rare 3-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1→3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application. |
| doi_str_mv | 10.1016/j.carres.2009.02.029 |
| format | Article |
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N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. 2S, 3S, 4S, and 6S represent 2-sulfate, 3-sulfate, 4-sulfate and 6-sulfate, respectively.
Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6
kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1→3GalNAc(6-sulfate), GlcAβ1→3GalNAc(4-sulfate), and GlcAβ1→3GalNAc(4,6-disulfate) as the major ones, significant amounts of rare 3-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1→3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/j.carres.2009.02.029</identifier><identifier>PMID: 19344892</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acetylgalactosamine - chemistry ; Animals ; Chondroitin sulfate ; Chondroitin Sulfates - chemistry ; Chondroitin Sulfates - isolation & purification ; Chromatography, High Pressure Liquid ; Decapodiformes - metabolism ; Glucuronates - chemistry ; Glycosaminoglycan ; Growth factor ; Liver - metabolism ; Molecular Structure ; Neurite outgrowth ; Squid liver ; Surface Plasmon Resonance</subject><ispartof>Carbohydrate research, 2009-08, Vol.344 (12), p.1526-1532</ispartof><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-75f09eb018e76a7e34d3bc40ed76ce61feec38f0d117ab23897024de78d83de13</citedby><cites>FETCH-LOGICAL-c516t-75f09eb018e76a7e34d3bc40ed76ce61feec38f0d117ab23897024de78d83de13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008621509000822$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19344892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shetty, Ajaya Kumar</creatorcontrib><creatorcontrib>Kobayashi, Takanari</creatorcontrib><creatorcontrib>Mizumoto, Shuji</creatorcontrib><creatorcontrib>Narumi, Masaki</creatorcontrib><creatorcontrib>Kudo, Yoshiaki</creatorcontrib><creatorcontrib>Yamada, Shuhei</creatorcontrib><creatorcontrib>Sugahara, Kazuyuki</creatorcontrib><title>Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4 ,6 -disulfate) and glucuronate(3 -sulfate) residues</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>Unique disaccharide composition of novel chondroitin sulfate from squid liver integument rich in
N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. 2S, 3S, 4S, and 6S represent 2-sulfate, 3-sulfate, 4-sulfate and 6-sulfate, respectively.
Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6
kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1→3GalNAc(6-sulfate), GlcAβ1→3GalNAc(4-sulfate), and GlcAβ1→3GalNAc(4,6-disulfate) as the major ones, significant amounts of rare 3-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1→3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.</description><subject>Acetylgalactosamine - chemistry</subject><subject>Animals</subject><subject>Chondroitin sulfate</subject><subject>Chondroitin Sulfates - chemistry</subject><subject>Chondroitin Sulfates - isolation & purification</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Decapodiformes - metabolism</subject><subject>Glucuronates - chemistry</subject><subject>Glycosaminoglycan</subject><subject>Growth factor</subject><subject>Liver - metabolism</subject><subject>Molecular Structure</subject><subject>Neurite outgrowth</subject><subject>Squid liver</subject><subject>Surface Plasmon Resonance</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UdFKHDEUDaWlrto_KCVPRcFZk8lsknkRiqgVpH2x0LeQTe6sWTKJJpkF-0_9x0Znad8KFy733HPP4XIQ-kjJkhLKz7dLo1OCvGwJ6ZekrdW_QQsqBWu6lv98ixaEENnwlq4O0GHO2zoSLvh7dEB71nWybxfo922OXhcXA9bBYvOgkzYFkvs1g3HAGoe4A193MdgUXXEB58kPugAeUhxxfpqcxd7tIGEXCmymEULByZmHOuNvjTZQnv1G-yodsx5dgJMOn3HcWLdXOn213_jJTCmGCpww3Pzd1TednSAfo3eD9hk-7PsR-nF9dX_5tbn7fnN7-eWuMSvKSyNWA-lhTagEwbUA1lm2Nh0BK7gBTgcAw-RALKVCr1sme0HazoKQVjILlB2hz7PuY4pP1beo0WUD3usAccqKi5WknIhK7GaiSTHnBIN6TG7U6VlRol5iUls1x6ReYlKkrdXXs097_Wk9gv13tM-lEi5mAtQvdw6SysZBMGBdAlOUje7_Dn8A9V6pnA</recordid><startdate>20090817</startdate><enddate>20090817</enddate><creator>Shetty, Ajaya Kumar</creator><creator>Kobayashi, Takanari</creator><creator>Mizumoto, Shuji</creator><creator>Narumi, Masaki</creator><creator>Kudo, Yoshiaki</creator><creator>Yamada, Shuhei</creator><creator>Sugahara, Kazuyuki</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090817</creationdate><title>Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4 ,6 -disulfate) and glucuronate(3 -sulfate) residues</title><author>Shetty, Ajaya Kumar ; Kobayashi, Takanari ; Mizumoto, Shuji ; Narumi, Masaki ; Kudo, Yoshiaki ; Yamada, Shuhei ; Sugahara, Kazuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-75f09eb018e76a7e34d3bc40ed76ce61feec38f0d117ab23897024de78d83de13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Acetylgalactosamine - chemistry</topic><topic>Animals</topic><topic>Chondroitin sulfate</topic><topic>Chondroitin Sulfates - chemistry</topic><topic>Chondroitin Sulfates - isolation & purification</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Decapodiformes - metabolism</topic><topic>Glucuronates - chemistry</topic><topic>Glycosaminoglycan</topic><topic>Growth factor</topic><topic>Liver - metabolism</topic><topic>Molecular Structure</topic><topic>Neurite outgrowth</topic><topic>Squid liver</topic><topic>Surface Plasmon Resonance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shetty, Ajaya Kumar</creatorcontrib><creatorcontrib>Kobayashi, Takanari</creatorcontrib><creatorcontrib>Mizumoto, Shuji</creatorcontrib><creatorcontrib>Narumi, Masaki</creatorcontrib><creatorcontrib>Kudo, Yoshiaki</creatorcontrib><creatorcontrib>Yamada, Shuhei</creatorcontrib><creatorcontrib>Sugahara, Kazuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shetty, Ajaya Kumar</au><au>Kobayashi, Takanari</au><au>Mizumoto, Shuji</au><au>Narumi, Masaki</au><au>Kudo, Yoshiaki</au><au>Yamada, Shuhei</au><au>Sugahara, Kazuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4 ,6 -disulfate) and glucuronate(3 -sulfate) residues</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2009-08-17</date><risdate>2009</risdate><volume>344</volume><issue>12</issue><spage>1526</spage><epage>1532</epage><pages>1526-1532</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>Unique disaccharide composition of novel chondroitin sulfate from squid liver integument rich in
N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues. 2S, 3S, 4S, and 6S represent 2-sulfate, 3-sulfate, 4-sulfate and 6-sulfate, respectively.
Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6
kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1→3GalNAc(6-sulfate), GlcAβ1→3GalNAc(4-sulfate), and GlcAβ1→3GalNAc(4,6-disulfate) as the major ones, significant amounts of rare 3-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1→3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19344892</pmid><doi>10.1016/j.carres.2009.02.029</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylgalactosamine - chemistry Animals Chondroitin sulfate Chondroitin Sulfates - chemistry Chondroitin Sulfates - isolation & purification Chromatography, High Pressure Liquid Decapodiformes - metabolism Glucuronates - chemistry Glycosaminoglycan Growth factor Liver - metabolism Molecular Structure Neurite outgrowth Squid liver Surface Plasmon Resonance |
| title | Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4 ,6 -disulfate) and glucuronate(3 -sulfate) residues |
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