Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120
Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate iron metabolism with several environmental stress responses. The regulatory network that governs Fur proteins is rather complex. Control at several stages from gene transcription to post-translational b...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular biology 2006-01, Vol.355 (3), p.325-334 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 334 |
|---|---|
| container_issue | 3 |
| container_start_page | 325 |
| container_title | Journal of molecular biology |
| container_volume | 355 |
| creator | Hernández, José A. Muro-Pastor, Alicia M. Flores, Enrique Bes, M. Teresa Peleato, M. Luisa Fillat, María F. |
| description | Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate iron metabolism with several environmental stress responses. The regulatory network that governs Fur proteins is rather complex. Control at several stages from gene transcription to post-translational binding of different ligands has been reported in Fur from
Escherichia coli. In the nitrogen-fixing cyanobacterium
Anabaena sp. strain PCC 7120 FurA is the product of open reading frame
all1691 that is located between
sigC and
alr1690, the latter encoding a putative cell wall-binding protein.
Anabaena FurA is an autoregulated protein whose expression increases slightly under iron deprivation. Northern blot analysis of
furA expression showed an unexpected transcription pattern that consisted of two transcripts. The short transcript corresponded to
furA mRNA, whereas the longer transcript contained the
alr1690 mRNA and a large region that overlapped the complete
furA gene and was complementary to the
furA mRNA. Increased expression of FurA in a mutant unable to produce the longer message showed that this transcript acted as an antisense RNA (α-
furA RNA) interfering with
furA transcript translation thus contributing to determine cellular levels of FurA protein. |
| doi_str_mv | 10.1016/j.jmb.2005.10.079 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67579265</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022283605013410</els_id><sourcerecordid>20241907</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-d201d429a88d5ee56000a5547524a50c5b00d6355e5c2e16e894366e98945da03</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVpaDZpf0AvQafe7IxkSZbpaTH5gpCE0vYqZGlMtaztrWQH8u-jZRdyS07DzDzzMjyEfGdQMmDqclNuhq7kADL3JdTNJ7JioJtCq0p_JisAzguuK3VKzlLaQAYrob-QU6YqLmomV-TvncdxDn1wdg7TSKeeWtovcU1dSHSdVwnHhPTXw5qGkc7_kLYvdpw662aMYRkyYzuLo6VpV9KntqU14_CVnPR2m_DbsZ6TP9dXv9vb4v7x5q5d3xdOCD0XngPzgjdWay8RpcovWilFLbmwEpzsALyqpETpODKFuhGVUtjkKr2F6pz8OOTu4vR_wTSbISSH260dcVqSUbWsG67khyAHLlgDdQbZAXRxSilib3YxDDa-GAZmb91sTLZu9tb3o2w931wcw5duQP92cdScgZ8HALOL54DRJBdwdOhDRDcbP4V34l8BFsOOlg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20241907</pqid></control><display><type>article</type><title>Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Hernández, José A. ; Muro-Pastor, Alicia M. ; Flores, Enrique ; Bes, M. Teresa ; Peleato, M. Luisa ; Fillat, María F.</creator><creatorcontrib>Hernández, José A. ; Muro-Pastor, Alicia M. ; Flores, Enrique ; Bes, M. Teresa ; Peleato, M. Luisa ; Fillat, María F.</creatorcontrib><description>Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate iron metabolism with several environmental stress responses. The regulatory network that governs Fur proteins is rather complex. Control at several stages from gene transcription to post-translational binding of different ligands has been reported in Fur from
Escherichia coli. In the nitrogen-fixing cyanobacterium
Anabaena sp. strain PCC 7120 FurA is the product of open reading frame
all1691 that is located between
sigC and
alr1690, the latter encoding a putative cell wall-binding protein.
Anabaena FurA is an autoregulated protein whose expression increases slightly under iron deprivation. Northern blot analysis of
furA expression showed an unexpected transcription pattern that consisted of two transcripts. The short transcript corresponded to
furA mRNA, whereas the longer transcript contained the
alr1690 mRNA and a large region that overlapped the complete
furA gene and was complementary to the
furA mRNA. Increased expression of FurA in a mutant unable to produce the longer message showed that this transcript acted as an antisense RNA (α-
furA RNA) interfering with
furA transcript translation thus contributing to determine cellular levels of FurA protein.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2005.10.079</identifier><identifier>PMID: 16324715</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anabaena ; Anabaena - genetics ; Anabaena - metabolism ; antisense RNA ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Cyanobacteria ; Cyanophyta ; Escherichia coli ; furA ; Gene Expression Regulation, Bacterial ; Iron - metabolism ; iron stress ; Mutation ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Antisense - genetics ; RNA, Antisense - metabolism ; Transcription, Genetic</subject><ispartof>Journal of molecular biology, 2006-01, Vol.355 (3), p.325-334</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-d201d429a88d5ee56000a5547524a50c5b00d6355e5c2e16e894366e98945da03</citedby><cites>FETCH-LOGICAL-c448t-d201d429a88d5ee56000a5547524a50c5b00d6355e5c2e16e894366e98945da03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmb.2005.10.079$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16324715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández, José A.</creatorcontrib><creatorcontrib>Muro-Pastor, Alicia M.</creatorcontrib><creatorcontrib>Flores, Enrique</creatorcontrib><creatorcontrib>Bes, M. Teresa</creatorcontrib><creatorcontrib>Peleato, M. Luisa</creatorcontrib><creatorcontrib>Fillat, María F.</creatorcontrib><title>Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate iron metabolism with several environmental stress responses. The regulatory network that governs Fur proteins is rather complex. Control at several stages from gene transcription to post-translational binding of different ligands has been reported in Fur from
Escherichia coli. In the nitrogen-fixing cyanobacterium
Anabaena sp. strain PCC 7120 FurA is the product of open reading frame
all1691 that is located between
sigC and
alr1690, the latter encoding a putative cell wall-binding protein.
Anabaena FurA is an autoregulated protein whose expression increases slightly under iron deprivation. Northern blot analysis of
furA expression showed an unexpected transcription pattern that consisted of two transcripts. The short transcript corresponded to
furA mRNA, whereas the longer transcript contained the
alr1690 mRNA and a large region that overlapped the complete
furA gene and was complementary to the
furA mRNA. Increased expression of FurA in a mutant unable to produce the longer message showed that this transcript acted as an antisense RNA (α-
furA RNA) interfering with
furA transcript translation thus contributing to determine cellular levels of FurA protein.</description><subject>Anabaena</subject><subject>Anabaena - genetics</subject><subject>Anabaena - metabolism</subject><subject>antisense RNA</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cyanobacteria</subject><subject>Cyanophyta</subject><subject>Escherichia coli</subject><subject>furA</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Iron - metabolism</subject><subject>iron stress</subject><subject>Mutation</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Antisense - genetics</subject><subject>RNA, Antisense - metabolism</subject><subject>Transcription, Genetic</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpaDZpf0AvQafe7IxkSZbpaTH5gpCE0vYqZGlMtaztrWQH8u-jZRdyS07DzDzzMjyEfGdQMmDqclNuhq7kADL3JdTNJ7JioJtCq0p_JisAzguuK3VKzlLaQAYrob-QU6YqLmomV-TvncdxDn1wdg7TSKeeWtovcU1dSHSdVwnHhPTXw5qGkc7_kLYvdpw662aMYRkyYzuLo6VpV9KntqU14_CVnPR2m_DbsZ6TP9dXv9vb4v7x5q5d3xdOCD0XngPzgjdWay8RpcovWilFLbmwEpzsALyqpETpODKFuhGVUtjkKr2F6pz8OOTu4vR_wTSbISSH260dcVqSUbWsG67khyAHLlgDdQbZAXRxSilib3YxDDa-GAZmb91sTLZu9tb3o2w931wcw5duQP92cdScgZ8HALOL54DRJBdwdOhDRDcbP4V34l8BFsOOlg</recordid><startdate>20060120</startdate><enddate>20060120</enddate><creator>Hernández, José A.</creator><creator>Muro-Pastor, Alicia M.</creator><creator>Flores, Enrique</creator><creator>Bes, M. Teresa</creator><creator>Peleato, M. Luisa</creator><creator>Fillat, María F.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060120</creationdate><title>Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120</title><author>Hernández, José A. ; Muro-Pastor, Alicia M. ; Flores, Enrique ; Bes, M. Teresa ; Peleato, M. Luisa ; Fillat, María F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-d201d429a88d5ee56000a5547524a50c5b00d6355e5c2e16e894366e98945da03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anabaena</topic><topic>Anabaena - genetics</topic><topic>Anabaena - metabolism</topic><topic>antisense RNA</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Cyanobacteria</topic><topic>Cyanophyta</topic><topic>Escherichia coli</topic><topic>furA</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Iron - metabolism</topic><topic>iron stress</topic><topic>Mutation</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Antisense - genetics</topic><topic>RNA, Antisense - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández, José A.</creatorcontrib><creatorcontrib>Muro-Pastor, Alicia M.</creatorcontrib><creatorcontrib>Flores, Enrique</creatorcontrib><creatorcontrib>Bes, M. Teresa</creatorcontrib><creatorcontrib>Peleato, M. Luisa</creatorcontrib><creatorcontrib>Fillat, María F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández, José A.</au><au>Muro-Pastor, Alicia M.</au><au>Flores, Enrique</au><au>Bes, M. Teresa</au><au>Peleato, M. Luisa</au><au>Fillat, María F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2006-01-20</date><risdate>2006</risdate><volume>355</volume><issue>3</issue><spage>325</spage><epage>334</epage><pages>325-334</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate iron metabolism with several environmental stress responses. The regulatory network that governs Fur proteins is rather complex. Control at several stages from gene transcription to post-translational binding of different ligands has been reported in Fur from
Escherichia coli. In the nitrogen-fixing cyanobacterium
Anabaena sp. strain PCC 7120 FurA is the product of open reading frame
all1691 that is located between
sigC and
alr1690, the latter encoding a putative cell wall-binding protein.
Anabaena FurA is an autoregulated protein whose expression increases slightly under iron deprivation. Northern blot analysis of
furA expression showed an unexpected transcription pattern that consisted of two transcripts. The short transcript corresponded to
furA mRNA, whereas the longer transcript contained the
alr1690 mRNA and a large region that overlapped the complete
furA gene and was complementary to the
furA mRNA. Increased expression of FurA in a mutant unable to produce the longer message showed that this transcript acted as an antisense RNA (α-
furA RNA) interfering with
furA transcript translation thus contributing to determine cellular levels of FurA protein.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16324715</pmid><doi>10.1016/j.jmb.2005.10.079</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 2006-01, Vol.355 (3), p.325-334 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67579265 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anabaena Anabaena - genetics Anabaena - metabolism antisense RNA Bacterial Proteins - genetics Bacterial Proteins - metabolism Cyanobacteria Cyanophyta Escherichia coli furA Gene Expression Regulation, Bacterial Iron - metabolism iron stress Mutation Repressor Proteins - genetics Repressor Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Antisense - genetics RNA, Antisense - metabolism Transcription, Genetic |
| title | Identification of a furA cis Antisense RNA in the Cyanobacterium Anabaena sp. PCC 7120 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T11%3A22%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20furA%20cis%20Antisense%20RNA%20in%20the%20Cyanobacterium%20Anabaena%20sp.%20PCC%207120&rft.jtitle=Journal%20of%20molecular%20biology&rft.au=Hern%C3%A1ndez,%20Jos%C3%A9%20A.&rft.date=2006-01-20&rft.volume=355&rft.issue=3&rft.spage=325&rft.epage=334&rft.pages=325-334&rft.issn=0022-2836&rft.eissn=1089-8638&rft_id=info:doi/10.1016/j.jmb.2005.10.079&rft_dat=%3Cproquest_cross%3E20241907%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20241907&rft_id=info:pmid/16324715&rft_els_id=S0022283605013410&rfr_iscdi=true |