Tetravalent neutralizing antibody response against four dengue serotypes by a single chimeric dengue envelope antigen

We employed DNA shuffling and screening technologies to develop a single recombinant dengue envelope (E) antigen capable of inducing neutralizing antibodies against all four antigenically distinct dengue serotypes. By DNA shuffling of codon-optimized dengue 1–4 E genes, we created a panel of novel c...

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Veröffentlicht in:	Vaccine 2006-01, Vol.24 (3), p.335-344
Hauptverfasser:	Apt, Doris, Raviprakash, Kanakatte, Brinkman, Alice, Semyonov, Andrey, Yang, Shumin, Skinner, Craig, Diehl, Lori, Lyons, Richard, Porter, Kevin, Punnonen, Juha
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - biosynthesis Antibodies, Viral - immunology Antigen Applied microbiology Arboviroses Biological and medical sciences Blotting, Western Cell Line Cloning Cross-neutralizing antibodies Dengue - immunology Dengue - prevention & control Dengue - virology Dengue fever Dengue fevers Dengue virus Dengue Virus - immunology Dengue virus type 2 Deoxyribonucleic acid Directed Molecular Evolution DNA DNA shuffling Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Library Human viral diseases Infections Infectious diseases Interferon-gamma Laboratory animals Medical sciences Mice Mice, Inbred BALB C Microbiology Miscellaneous Molecular weight Multivalent antigen Neutralization Tests Plasmids - genetics Proteins Recombinant Fusion Proteins - immunology T-Lymphocytes - immunology Tetravalent Transfection Tropical diseases Tropical viral diseases Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - immunology Vaccines, Synthetic - immunology Vector-borne diseases Viral diseases Viral Envelope Proteins - immunology Viral Vaccines - immunology Virology Viruses
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