Mesenchymal stem cells from multiple myeloma patients display distinct genomic profile as compared with those from normal donors

It is an open question whether in multiple myeloma (MM) bone marrow stromal cells contain genomic alterations, which may contribute to the pathogenesis of the disease. We conducted an array-based comparative genomic hybridization (array-CGH) analysis to compare the extent of unbalanced genomic alter...

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Veröffentlicht in:	Leukemia 2009-08, Vol.23 (8), p.1515-1527
Hauptverfasser:	Garayoa, M, Garcia, J L, Santamaria, C, Garcia-Gomez, A, Blanco, J F, Pandiella, A, Hernández, J M, Sanchez-Guijo, F M, del Cañizo, M-C, Gutiérrez, N C, San Miguel, J F
Format:	Artikel
Sprache:	eng
Schlagworte:	Abnormalities Adult Aged Aged, 80 and over Arrays Biological and medical sciences Bone marrow Bone Marrow - pathology Bone Marrow Cells - chemistry Bone marrow transplantation Cancer Research Cell culture Cell Lineage Cells, Cultured - chemistry Cluster Analysis Comparative analysis Comparative Genomic Hybridization Critical Care Medicine Cytogenetics Deregulation Female Fluorescence Fluorescence in situ hybridization Gene Dosage Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Genomics Hematologic and hematopoietic diseases Hematology Humans Immunodeficiencies. Immunoglobulinopathies Immunoglobulinopathies Immunopathology In Situ Hybridization, Fluorescence Intensive Internal Medicine Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Medicine Medicine & Public Health Mesenchymal stem cells Mesenchymal Stromal Cells - chemistry Middle Aged Multiple myeloma Multiple Myeloma - genetics Multiple Myeloma - pathology Neoplastic Stem Cells - chemistry Oligonucleotide Array Sequence Analysis Oncology original-article Pathogenesis Physiological aspects Plasma cells Plasma Cells - chemistry Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Stem cell transplantation Stem cells Stromal cells Tumor Cells, Cultured - chemistry
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Zusammenfassung:	It is an open question whether in multiple myeloma (MM) bone marrow stromal cells contain genomic alterations, which may contribute to the pathogenesis of the disease. We conducted an array-based comparative genomic hybridization (array-CGH) analysis to compare the extent of unbalanced genomic alterations in mesenchymal stem cells from 21 myeloma patients (MM-MSCs) and 12 normal donors (ND-MSCs) after in vitro culture expansion. Whereas ND-MSCs were devoid of genomic imbalances, several non-recurrent chromosomal gains and losses (>1 Mb size) were detected in MM-MSCs. Using real-time reverse transcription PCR, we found correlative deregulated expression for five genes encoded in regions for which genomic imbalances were detected using array-CGH. In addition, only MM-MSCs showed a specific pattern of ‘hot-spot’ regions with discrete (
ISSN:	0887-6924 1476-5551
DOI:	10.1038/leu.2009.65