Humanization of chicken monoclonal antibody using phage-display system

We describe a simple method for humanizing chicken monoclonal antibody (mAb). Humanization of mAbs by simple CDR-grafting often results in loss of affinity because certain framework residues of the antibody variable regions can participate in antigen–antibody interaction. In this study, humanization...

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Veröffentlicht in:	Molecular immunology 2006-02, Vol.43 (6), p.634-642
Hauptverfasser:	Nishibori, Nahoko, Horiuchi, Hiroyuki, Furusawa, Shuichi, Matsuda, Haruo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - genetics Antibody Affinity Chicken Chickens Cloning, Molecular Complementarity Determining Regions - genetics Humanization Humanized antibody Humans Immunoglobulin Fab Fragments Immunoglobulin Variable Region Methods Peptide Library Protein Engineering
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