Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells
Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. T...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2005, Vol.28(4), pp.667-670 |
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| creator | Sekiya, Nobuyasu Hikiami, Hiroaki Yokoyama, Koichi Kouta, Kazufumi Sakakibara, Iwao Shimada, Yutaka Terasawa, Katsutoshi |
| description | Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo. |
| doi_str_mv | 10.1248/bpb.28.667 |
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The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.28.667</identifier><identifier>PMID: 15802807</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Alkaloids - pharmacology ; Animals ; Antioxidants - pharmacology ; antioxidative effect ; Benzylisoquinolines - pharmacology ; Berberine - analogs & derivatives ; Berberine - pharmacology ; Dose-Response Relationship, Drug ; Free Radical Scavengers - pharmacology ; Free Radicals - metabolism ; Hemolysis - drug effects ; In Vitro Techniques ; Male ; Molecular Structure ; Plant Extracts - pharmacology ; Rats ; Rats, Wistar ; reactive oxygen specie ; Stephania tetrandra ; Stephania tetrandra - chemistry ; Time Factors</subject><ispartof>Biological and Pharmaceutical Bulletin, 2005, Vol.28(4), pp.667-670</ispartof><rights>2005 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-b7c06d26c2394fdc99ad07e0c80bac51aec4c4e20c3e888e482a4460019d03803</citedby><cites>FETCH-LOGICAL-c471t-b7c06d26c2394fdc99ad07e0c80bac51aec4c4e20c3e888e482a4460019d03803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15802807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sekiya, Nobuyasu</creatorcontrib><creatorcontrib>Hikiami, Hiroaki</creatorcontrib><creatorcontrib>Yokoyama, Koichi</creatorcontrib><creatorcontrib>Kouta, Kazufumi</creatorcontrib><creatorcontrib>Sakakibara, Iwao</creatorcontrib><creatorcontrib>Shimada, Yutaka</creatorcontrib><creatorcontrib>Terasawa, Katsutoshi</creatorcontrib><title>Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.</description><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>antioxidative effect</subject><subject>Benzylisoquinolines - pharmacology</subject><subject>Berberine - analogs & derivatives</subject><subject>Berberine - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Free Radicals - metabolism</subject><subject>Hemolysis - drug effects</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Molecular Structure</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>reactive oxygen specie</subject><subject>Stephania tetrandra</subject><subject>Stephania tetrandra - chemistry</subject><subject>Time Factors</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkFGLEzEQx4MoXj198QNIQPBB2N4km91kn0RLzyv0KPT0OWST2euW7aYm2Yd-e6Otd-DLDMz85s_wI-Q9gznjQt20x3bO1byu5QsyY6WQRcVZ9ZLMoGGqqFmlrsibGPcAIIGXr8lVHgFXIGfkcTXu-rZPPpzosuvQpkh9Rx8SHndm7A1NmIIZXTD0YU7vN5vtkvqR3gZEujWut2YoVqObLDq6PsX-7_XWJLrNg2-D944ucBjiW_KqM0PEd5d-TX7eLn8s7or15vtq8XVdWCFZKlppoXa8trxsROds0xgHEsEqaI2tmEErrEAOtkSlFArFjRA1AGsclArKa_LpnHsM_teEMelDH23-wIzop6hrWUlR1iKDH_8D934KY_5NMyGaUmS1LFOfz5QNPsaAnT6G_mDCSTPQf-TrLF9zpbP8DH-4RE7tAd0zerGdgS9nYB-TecQnwITU2wH_ZYlzyZFPG7szQeNY_gZXjZRL</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Sekiya, Nobuyasu</creator><creator>Hikiami, Hiroaki</creator><creator>Yokoyama, Koichi</creator><creator>Kouta, Kazufumi</creator><creator>Sakakibara, Iwao</creator><creator>Shimada, Yutaka</creator><creator>Terasawa, Katsutoshi</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells</title><author>Sekiya, Nobuyasu ; Hikiami, Hiroaki ; Yokoyama, Koichi ; Kouta, Kazufumi ; Sakakibara, Iwao ; Shimada, Yutaka ; Terasawa, Katsutoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-b7c06d26c2394fdc99ad07e0c80bac51aec4c4e20c3e888e482a4460019d03803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Alkaloids - pharmacology</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>antioxidative effect</topic><topic>Benzylisoquinolines - pharmacology</topic><topic>Berberine - analogs & derivatives</topic><topic>Berberine - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Free Radicals - metabolism</topic><topic>Hemolysis - drug effects</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Molecular Structure</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>reactive oxygen specie</topic><topic>Stephania tetrandra</topic><topic>Stephania tetrandra - chemistry</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sekiya, Nobuyasu</creatorcontrib><creatorcontrib>Hikiami, Hiroaki</creatorcontrib><creatorcontrib>Yokoyama, Koichi</creatorcontrib><creatorcontrib>Kouta, Kazufumi</creatorcontrib><creatorcontrib>Sakakibara, Iwao</creatorcontrib><creatorcontrib>Shimada, Yutaka</creatorcontrib><creatorcontrib>Terasawa, Katsutoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sekiya, Nobuyasu</au><au>Hikiami, Hiroaki</au><au>Yokoyama, Koichi</au><au>Kouta, Kazufumi</au><au>Sakakibara, Iwao</au><au>Shimada, Yutaka</au><au>Terasawa, Katsutoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>28</volume><issue>4</issue><spage>667</spage><epage>670</epage><pages>667-670</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15802807</pmid><doi>10.1248/bpb.28.667</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaloids - pharmacology Animals Antioxidants - pharmacology antioxidative effect Benzylisoquinolines - pharmacology Berberine - analogs & derivatives Berberine - pharmacology Dose-Response Relationship, Drug Free Radical Scavengers - pharmacology Free Radicals - metabolism Hemolysis - drug effects In Vitro Techniques Male Molecular Structure Plant Extracts - pharmacology Rats Rats, Wistar reactive oxygen specie Stephania tetrandra Stephania tetrandra - chemistry Time Factors |
| title | Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells |
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