Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe

A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-...

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Veröffentlicht in:	Analytical biochemistry 2005-05, Vol.340 (1), p.66-73
Hauptverfasser:	Wu, Meng, Lin, Zhihong, Schäferling, Michael, Dürkop, Axel, Wolfbeis, Otto S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques Enzyme activity assay Enzyme imaging Europium - chemistry Fluorescent probe Glucose oxidase Glucose Oxidase - metabolism Hydrogen Peroxide - analysis Hydrogen Peroxide - metabolism Hydrogen-Ion Concentration Immunoassay Kinetics Molecular Probes - chemistry Sensitivity and Specificity Spectrometry, Fluorescence Tetracycline - chemistry Time Factors Time-resolved imaging
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