Point mutation detection with the sandwich method employing hydrogel nanospheres by the surface plasmon resonance imaging technique

We propose a surface modification procedure to construct DNA arrays for use in surface plasmon resonance (SPR) imaging studies for the highly sensitive detection of a K- ras point mutation, enhanced with hydrogel nanospheres. A homobifunctional alkane dithiol was adsorbed on Au film to obtain the th...

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Veröffentlicht in:	Analytical biochemistry 2005-04, Vol.339 (2), p.328-337
Hauptverfasser:	Okumura, Aya, Sato, Yasunobu, Kyo, Motoki, Kawaguchi, Haruma
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biotinylation DNA array DNA Probes - chemistry Hydrogel nanosphere Hydrogels K- ras point mutation Nanotubes Nucleic Acid Amplification Techniques - methods Nucleic Acid Hybridization Point Mutation Sandwich method Surface Plasmon Resonance - methods Surface plasmon resonance imaging
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