Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles
We have developed a simple scheme for the isolation of parasitophorous vacuoles (PVs) that harbor Leishmania parasites. This scheme exploits the observation that PVs display endoplasmic reticulum molecules, including the transmembrane protein calnexin. The presence of calnexin at the surface of the...
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| Veröffentlicht in: | Microbial pathogenesis 2005-04, Vol.38 (4), p.139-145 |
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| container_title | Microbial pathogenesis |
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| creator | Kima, Peter E. Dunn, Waltraud |
| description | We have developed a simple scheme for the isolation of parasitophorous vacuoles (PVs) that harbor
Leishmania parasites. This scheme exploits the observation that PVs display endoplasmic reticulum molecules, including the transmembrane protein calnexin. The presence of calnexin at the surface of the PVs distinguishes them from late endosomal vesicles of comparable density. As a result, PVs can be isolated by calnexin affinity selection from an enriched PV fraction obtained by sucrose density fractionation. |
| doi_str_mv | 10.1016/j.micpath.2004.11.003 |
| format | Article |
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Leishmania parasites. This scheme exploits the observation that PVs display endoplasmic reticulum molecules, including the transmembrane protein calnexin. The presence of calnexin at the surface of the PVs distinguishes them from late endosomal vesicles of comparable density. As a result, PVs can be isolated by calnexin affinity selection from an enriched PV fraction obtained by sucrose density fractionation.</description><subject>Amastigotes</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calnexin</subject><subject>Calnexin - biosynthesis</subject><subject>Calnexin - chemistry</subject><subject>Cathepsins</subject><subject>Cell Line</subject><subject>Centrifugation, Density Gradient</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Leishmania</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmania - metabolism</subject><subject>Leishmania pifanoi</subject><subject>Macrophages - parasitology</subject><subject>Macrophages - ultrastructure</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Parasitophorous vacuole</subject><subject>Phagosomes - metabolism</subject><subject>Phagosomes - parasitology</subject><subject>Phagosomes - ultrastructure</subject><subject>Promastigotes</subject><subject>Subcellular Fractions - metabolism</subject><subject>Subcellular Fractions - parasitology</subject><subject>Vacuoles - parasitology</subject><subject>Vacuoles - ultrastructure</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFq3DAQhkVpaLZpH6HFl_ZmdyRbsnUqJaRNYKGX5NSDkOVxVottuRpv2Lx9FNaQ48KALt-v-ZmPsS8cCg5c_dgXo3ezXXaFAKgKzguA8h3bcNAq5wKa92wDTSPyCjhcso9EewDQVak_sEsua103oDfs381xHoJf_PSYOTtMePRThsc5IpEPU5Zm3tnHQGFEypaQeQqDXTDboqfdaCdvs9lGS34J8y7EcKDsybpDGJA-sYveDoSf1_eKPfy-ub--zbd__9xd_9rmTgq-5C1o2eiuUq53qZaolbC1Aud45ayFVtXYuE6UPW87LaGrXUpUEpxVfVuqrrxi30__zjH8PyAtZvTkcBjshKmPUbVUpRDiLMh1CY2q9Hmwlum2SiZQnkAXA1HE3szRjzY-Gw7m1ZPZm9WTefVkODfJU8p9XRcc2hG7t9QqJgHfVsBS8tJHOzlPb5xSdSNVlbifJw7TgZ88RkPO4-Sw8xHdYrrgz1R5AeO0tXE</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Kima, Peter E.</creator><creator>Dunn, Waltraud</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles</title><author>Kima, Peter E. ; Dunn, Waltraud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-b09589d46cfc7972762a760cc14caa0b67e8cd23f1bd950d7cb09450ca6fb36d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amastigotes</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calnexin</topic><topic>Calnexin - biosynthesis</topic><topic>Calnexin - chemistry</topic><topic>Cathepsins</topic><topic>Cell Line</topic><topic>Centrifugation, Density Gradient</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Leishmania</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmania - metabolism</topic><topic>Leishmania pifanoi</topic><topic>Macrophages - parasitology</topic><topic>Macrophages - ultrastructure</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Fluorescence</topic><topic>Parasitophorous vacuole</topic><topic>Phagosomes - metabolism</topic><topic>Phagosomes - parasitology</topic><topic>Phagosomes - ultrastructure</topic><topic>Promastigotes</topic><topic>Subcellular Fractions - metabolism</topic><topic>Subcellular Fractions - parasitology</topic><topic>Vacuoles - parasitology</topic><topic>Vacuoles - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kima, Peter E.</creatorcontrib><creatorcontrib>Dunn, Waltraud</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kima, Peter E.</au><au>Dunn, Waltraud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>38</volume><issue>4</issue><spage>139</spage><epage>145</epage><pages>139-145</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>We have developed a simple scheme for the isolation of parasitophorous vacuoles (PVs) that harbor
Leishmania parasites. This scheme exploits the observation that PVs display endoplasmic reticulum molecules, including the transmembrane protein calnexin. The presence of calnexin at the surface of the PVs distinguishes them from late endosomal vesicles of comparable density. As a result, PVs can be isolated by calnexin affinity selection from an enriched PV fraction obtained by sucrose density fractionation.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>15797809</pmid><doi>10.1016/j.micpath.2004.11.003</doi><tpages>7</tpages></addata></record> |
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| ispartof | Microbial pathogenesis, 2005-04, Vol.38 (4), p.139-145 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amastigotes Animals Biological and medical sciences Calnexin Calnexin - biosynthesis Calnexin - chemistry Cathepsins Cell Line Centrifugation, Density Gradient Fundamental and applied biological sciences. Psychology Leishmania Leishmania - isolation & purification Leishmania - metabolism Leishmania pifanoi Macrophages - parasitology Macrophages - ultrastructure Mice Microbiology Microscopy, Electron, Transmission Microscopy, Fluorescence Parasitophorous vacuole Phagosomes - metabolism Phagosomes - parasitology Phagosomes - ultrastructure Promastigotes Subcellular Fractions - metabolism Subcellular Fractions - parasitology Vacuoles - parasitology Vacuoles - ultrastructure |
| title | Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles |
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