Antigen-Driven Evolution of B Lymphocytes in Coronary Atherosclerotic Plaques
Recent data indicated that adaptive immunity is involved in the process of atherogenesis. Oligoclonal recruitment of T lymphocytes has been described in coronary plaques of patients with acute coronary syndrome. However, the nature of immune response remains to be determined. In the present study, w...
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| Veröffentlicht in: | The Journal of immunology (1950) 2009-08, Vol.183 (4), p.2537-2544 |
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| container_title | The Journal of immunology (1950) |
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| creator | Burioni, Roberto Canducci, Filippo Saita, Diego Perotti, Mario Mancini, Nicasio De Marco, Donata Clementi, Nicola Chieffo, Alaide Denaro, Maurizio Cianflone, Domenico Manfredi, Angelo A Colombo, Antonio Maseri, Attilio Clementi, Massimo |
| description | Recent data indicated that adaptive immunity is involved in the process of atherogenesis. Oligoclonal recruitment of T lymphocytes has been described in coronary plaques of patients with acute coronary syndrome. However, the nature of immune response remains to be determined. In the present study, we examined the Ab response in six coronary plaques obtained by endoluminal directional atherectomy. The IgG1/kappa-coding gene repertoires of B lymphocytes present in circulating blood and in coronary plaques were cloned and analyzed. In all of the six plaques, we observed 1) a skewed usage of heavy and light IgG1/kappa Ab-coding genes, 2) an oligoclonal distribution of V(K), J(K), and V(H), D(H), and J(H) genes with overrepresentation of some rarely used IgG genes, and 3) the unequivocal signs of Ag-driven clonal expansion and evolution of B cells. The data document for the first time the presence of a local Ag-driven clonal evolution of B cells in human atherosclerotic plaques. |
| doi_str_mv | 10.4049/jimmunol.0901076 |
| format | Article |
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Oligoclonal recruitment of T lymphocytes has been described in coronary plaques of patients with acute coronary syndrome. However, the nature of immune response remains to be determined. In the present study, we examined the Ab response in six coronary plaques obtained by endoluminal directional atherectomy. The IgG1/kappa-coding gene repertoires of B lymphocytes present in circulating blood and in coronary plaques were cloned and analyzed. In all of the six plaques, we observed 1) a skewed usage of heavy and light IgG1/kappa Ab-coding genes, 2) an oligoclonal distribution of V(K), J(K), and V(H), D(H), and J(H) genes with overrepresentation of some rarely used IgG genes, and 3) the unequivocal signs of Ag-driven clonal expansion and evolution of B cells. The data document for the first time the presence of a local Ag-driven clonal evolution of B cells in human atherosclerotic plaques.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.0901076</identifier><identifier>PMID: 19635916</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Adult ; Aged ; Antigens, Bacterial - physiology ; Autoantigens - physiology ; B-Lymphocyte Subsets - immunology ; B-Lymphocyte Subsets - microbiology ; B-Lymphocyte Subsets - pathology ; Cell Proliferation ; Clone Cells ; Coronary Artery Disease - blood ; Coronary Artery Disease - immunology ; Coronary Artery Disease - pathology ; Evolution, Molecular ; Humans ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Immunoglobulin G - genetics ; Immunoglobulin kappa-Chains - biosynthesis ; Immunoglobulin kappa-Chains - blood ; Immunoglobulin kappa-Chains - genetics ; Male ; Middle Aged ; Multigene Family - immunology</subject><ispartof>The Journal of immunology (1950), 2009-08, Vol.183 (4), p.2537-2544</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-afb0da089030582b5e8ab887c5d8c6c043313524fa24190207eb1f9fc6774b4f3</citedby><cites>FETCH-LOGICAL-c437t-afb0da089030582b5e8ab887c5d8c6c043313524fa24190207eb1f9fc6774b4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19635916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burioni, Roberto</creatorcontrib><creatorcontrib>Canducci, Filippo</creatorcontrib><creatorcontrib>Saita, Diego</creatorcontrib><creatorcontrib>Perotti, Mario</creatorcontrib><creatorcontrib>Mancini, Nicasio</creatorcontrib><creatorcontrib>De Marco, Donata</creatorcontrib><creatorcontrib>Clementi, Nicola</creatorcontrib><creatorcontrib>Chieffo, Alaide</creatorcontrib><creatorcontrib>Denaro, Maurizio</creatorcontrib><creatorcontrib>Cianflone, Domenico</creatorcontrib><creatorcontrib>Manfredi, Angelo A</creatorcontrib><creatorcontrib>Colombo, Antonio</creatorcontrib><creatorcontrib>Maseri, Attilio</creatorcontrib><creatorcontrib>Clementi, Massimo</creatorcontrib><title>Antigen-Driven Evolution of B Lymphocytes in Coronary Atherosclerotic Plaques</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Recent data indicated that adaptive immunity is involved in the process of atherogenesis. Oligoclonal recruitment of T lymphocytes has been described in coronary plaques of patients with acute coronary syndrome. However, the nature of immune response remains to be determined. In the present study, we examined the Ab response in six coronary plaques obtained by endoluminal directional atherectomy. The IgG1/kappa-coding gene repertoires of B lymphocytes present in circulating blood and in coronary plaques were cloned and analyzed. In all of the six plaques, we observed 1) a skewed usage of heavy and light IgG1/kappa Ab-coding genes, 2) an oligoclonal distribution of V(K), J(K), and V(H), D(H), and J(H) genes with overrepresentation of some rarely used IgG genes, and 3) the unequivocal signs of Ag-driven clonal expansion and evolution of B cells. The data document for the first time the presence of a local Ag-driven clonal evolution of B cells in human atherosclerotic plaques.</description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Bacterial - physiology</subject><subject>Autoantigens - physiology</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>B-Lymphocyte Subsets - microbiology</subject><subject>B-Lymphocyte Subsets - pathology</subject><subject>Cell Proliferation</subject><subject>Clone Cells</subject><subject>Coronary Artery Disease - blood</subject><subject>Coronary Artery Disease - immunology</subject><subject>Coronary Artery Disease - pathology</subject><subject>Evolution, Molecular</subject><subject>Humans</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - genetics</subject><subject>Immunoglobulin kappa-Chains - biosynthesis</subject><subject>Immunoglobulin kappa-Chains - blood</subject><subject>Immunoglobulin kappa-Chains - genetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Multigene Family - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAURi0EoqWwM6FMMAWu38lYSnlIRTDAbDmu07py4hInrfrvSdUilnuX893HQegawz0Dlj-sXFV1dfD3kAMGKU7QEHMOqRAgTtEQgJAUSyEH6CLGFQAIIOwcDXAuKM-xGKL3cd26ha3Tp8ZtbJ1MN8F3rQt1EsrkMZntqvUymF1rY-LqZBKaUOtml4zbpW1CNL6vrTPJp9c_nY2X6KzUPtqrYx-h7-fp1-Q1nX28vE3Gs9QwKttUlwXMNWQ5UOAZKbjNdJFl0vB5ZoQBRimmnLBSE4ZzICBtgcu8NEJKVrCSjtDtYe66Cfu9rapcNNZ7XdvQRSUkF5gQ2oNwAE1_bWxsqdaNq_oPFAa1V6j-FKqjwj5yc5zdFZWd_weOznrg7gAs3WK5dY1VsdLe9zhW2-0WZ1QxRTiV9BfigHxF</recordid><startdate>20090815</startdate><enddate>20090815</enddate><creator>Burioni, Roberto</creator><creator>Canducci, Filippo</creator><creator>Saita, Diego</creator><creator>Perotti, Mario</creator><creator>Mancini, Nicasio</creator><creator>De Marco, Donata</creator><creator>Clementi, Nicola</creator><creator>Chieffo, Alaide</creator><creator>Denaro, Maurizio</creator><creator>Cianflone, Domenico</creator><creator>Manfredi, Angelo A</creator><creator>Colombo, Antonio</creator><creator>Maseri, Attilio</creator><creator>Clementi, Massimo</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090815</creationdate><title>Antigen-Driven Evolution of B Lymphocytes in Coronary Atherosclerotic Plaques</title><author>Burioni, Roberto ; 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Oligoclonal recruitment of T lymphocytes has been described in coronary plaques of patients with acute coronary syndrome. However, the nature of immune response remains to be determined. In the present study, we examined the Ab response in six coronary plaques obtained by endoluminal directional atherectomy. The IgG1/kappa-coding gene repertoires of B lymphocytes present in circulating blood and in coronary plaques were cloned and analyzed. In all of the six plaques, we observed 1) a skewed usage of heavy and light IgG1/kappa Ab-coding genes, 2) an oligoclonal distribution of V(K), J(K), and V(H), D(H), and J(H) genes with overrepresentation of some rarely used IgG genes, and 3) the unequivocal signs of Ag-driven clonal expansion and evolution of B cells. 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| subjects | Adult Aged Antigens, Bacterial - physiology Autoantigens - physiology B-Lymphocyte Subsets - immunology B-Lymphocyte Subsets - microbiology B-Lymphocyte Subsets - pathology Cell Proliferation Clone Cells Coronary Artery Disease - blood Coronary Artery Disease - immunology Coronary Artery Disease - pathology Evolution, Molecular Humans Immunoglobulin G - biosynthesis Immunoglobulin G - blood Immunoglobulin G - genetics Immunoglobulin kappa-Chains - biosynthesis Immunoglobulin kappa-Chains - blood Immunoglobulin kappa-Chains - genetics Male Middle Aged Multigene Family - immunology |
| title | Antigen-Driven Evolution of B Lymphocytes in Coronary Atherosclerotic Plaques |
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