Georgenia thermotolerans sp. nov., an actinobacterium isolated from forest soil

NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan Correspondence Moriyuki Hamada hamada-moriyuki{at}nite.go.jp A Gram-positive bacterium strain, designated TT02-04 T , was isolated from a forest soil sample in Iriomote Island, Japan, and its taxonomic position was investigated by using a polyphasic approach. The peptidoglycan type of this organism was found to be A4 and lysine was the diagnostic cell-wall diamino acid of the peptidoglycan. The only menaquinone was MK-8(H 4 ), and the major fatty acid was anteiso-C 15 : 0 . Galactose was detected as the cell-wall sugar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The DNA G+C content was 73.0 mol%. 16S rRNA gene sequencing studies indicated that strain TT02-04 T was closely related to the type strains of Georgenia ruanii (99.0 %) and Georgenia muralis (97.7 %). However, DNA–DNA hybridization results and phenotypic characteristics revealed that the strain differed from the currently recognized species of the genus Georgenia . Therefore, strain TT02-04 T represents a novel species of the genus Georgenia , for which the name Georgenia thermotolerans sp. nov. is proposed. The type strain is TT02-04 T (=NBRC 104148 T =DSM 21501 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TT02-04 T is AB436534 .