SREBP Pathway Responds to Sterols and Functions as an Oxygen Sensor in Fission Yeast
Cholesterol and fatty acid synthesis in mammals are controlled by SREBPs, a family of membrane bound transcription factors. Our studies identified homologs of SREBP, its binding partner SCAP, and the ER retention protein Insig in Schizosaccharomyces pombe, named sre1+, scp1+, and ins1+. Like SREBP,...
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| Veröffentlicht in: | Cell 2005-03, Vol.120 (6), p.831-842 |
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| creator | Hughes, Adam L. Todd, Bridget L. Espenshade, Peter J. |
| description | Cholesterol and fatty acid synthesis in mammals are controlled by SREBPs, a family of membrane bound transcription factors. Our studies identified homologs of SREBP, its binding partner SCAP, and the ER retention protein Insig in Schizosaccharomyces pombe, named sre1+, scp1+, and ins1+. Like SREBP, Sre1 is cleaved and activated in response to sterol depletion in a Scp1-dependent manner. Microarray analysis revealed that Sre1 activates sterol biosynthetic enzymes as in mammals, and, surprisingly, Sre1 also stimulates transcription of genes required for adaptation to hypoxia. Furthermore, Sre1 rapidly activates these target genes in response to low oxygen and is itself required for anaerobic growth. Based on these findings, we propose and test a model in which Sre1 and Scp1 monitor oxygen-dependent sterol synthesis as an indirect measure of oxygen supply and mediate a hypoxic response in fission yeast. |
| doi_str_mv | 10.1016/j.cell.2005.01.012 |
| format | Article |
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Based on these findings, we propose and test a model in which Sre1 and Scp1 monitor oxygen-dependent sterol synthesis as an indirect measure of oxygen supply and mediate a hypoxic response in fission yeast.</description><subject>Animals</subject><subject>CHO Cells</subject><subject>Cholesterol - biosynthesis</subject><subject>Cricetinae</subject><subject>Gene Expression Regulation, Fungal - physiology</subject><subject>Microarray Analysis</subject><subject>Oxygen - analysis</subject><subject>Oxygen - metabolism</subject><subject>Schizosaccharomyces - growth & development</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF2LEzEUhoMobl39A15IrrybmpOPyQx4o8tWhYVdtuuFVyGTnNGUNqnJdLX_3gwteKdwICR53pfDQ8hrYEtg0L7bLB1ut0vOmFoyqMOfkAWwXjcSNH9KFoz1vOlaLS_Ii1I2jLFOKfWcXIDSvRadWJCH9f31xzt6Z6cfv-yR3mPZp-gLnRJdT5jTtlAbPV0doptCivU2P9Db38fvGOkaY0mZhkhXoZT6T7-hLdNL8my024Kvzucl-bq6frj63Nzcfvpy9eGmcbJXUwOug54PykvhQWiQrtO95JrbEYa27uetAJB84MCVHv0wKHRc98gUylF4cUnennr3Of08YJnMLpTZiY2YDsW0WqlOCvVfEHQn-hZkBfkJdDmVknE0-xx2Nh8NMDNLNxsz58ws3TCow2vozbn9MOzQ_42cLVfg_QnAKuMxYDbFBYwOfcjoJuNT-Ff_H28ukU0</recordid><startdate>20050325</startdate><enddate>20050325</enddate><creator>Hughes, Adam L.</creator><creator>Todd, Bridget L.</creator><creator>Espenshade, Peter J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050325</creationdate><title>SREBP Pathway Responds to Sterols and Functions as an Oxygen Sensor in Fission Yeast</title><author>Hughes, Adam L. ; Todd, Bridget L. ; Espenshade, Peter J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-1c8192b5d43d13714c8794272af1b6738da31142b21257fdbb5ec279e05e4f3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>CHO Cells</topic><topic>Cholesterol - biosynthesis</topic><topic>Cricetinae</topic><topic>Gene Expression Regulation, Fungal - physiology</topic><topic>Microarray Analysis</topic><topic>Oxygen - analysis</topic><topic>Oxygen - metabolism</topic><topic>Schizosaccharomyces - growth & development</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hughes, Adam L.</creatorcontrib><creatorcontrib>Todd, Bridget L.</creatorcontrib><creatorcontrib>Espenshade, Peter J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hughes, Adam L.</au><au>Todd, Bridget L.</au><au>Espenshade, Peter J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SREBP Pathway Responds to Sterols and Functions as an Oxygen Sensor in Fission Yeast</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2005-03-25</date><risdate>2005</risdate><volume>120</volume><issue>6</issue><spage>831</spage><epage>842</epage><pages>831-842</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Cholesterol and fatty acid synthesis in mammals are controlled by SREBPs, a family of membrane bound transcription factors. 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| source | MEDLINE; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier) |
| subjects | Animals CHO Cells Cholesterol - biosynthesis Cricetinae Gene Expression Regulation, Fungal - physiology Microarray Analysis Oxygen - analysis Oxygen - metabolism Schizosaccharomyces - growth & development Schizosaccharomyces - metabolism Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - metabolism Transcription Factors - metabolism Transcription, Genetic |
| title | SREBP Pathway Responds to Sterols and Functions as an Oxygen Sensor in Fission Yeast |
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