Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants
In Arabidopsis, NPR1 (non-expressor of pathogenesis related genes 1, AtNPR1) functions downstream of salicylic acid (SA) and modulates the SA mediated systemic acquired resistance. It is also involved in a cross talk with the jasmonate pathway that is essential for resistance against herbivores and...
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| Veröffentlicht in: | Biotechnology letters 2009-09, Vol.31 (9), p.1343-1351 |
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| creator | Srinivasan, T Kumar, K. Raja Rajesh Meur, G Kirti, P. B |
| description | In Arabidopsis, NPR1 (non-expressor of pathogenesis related genes 1, AtNPR1) functions downstream of salicylic acid (SA) and modulates the SA mediated systemic acquired resistance. It is also involved in a cross talk with the jasmonate pathway that is essential for resistance against herbivores and necrotrophic pathogens. Overexpression of AtNPR1 in transgenic plants resulted in enhanced disease resistance. Recently, tobacco transgenic plants expressing AtNPR1 were shown to be tolerant to the early instars of Spodoptera litura (Meur et al., Physiol Plant 133:765-775, 2008). In this communication, we show that the heterologous expression of AtNPR1 in tobacco has also enhanced the oxidative stress tolerance. The transgenic plants exhibited enhanced tolerance to the treatment with methyl viologen. This tolerance was associated with the constitutive upregulation of PR1, PR2 (glucanase), PR5 (thaumatin like protein), ascorbate peroxidase (APX) and Cu²⁺/Zn²⁺ superoxide dismutase (SOD). This is the first demonstration of the novel function of heterologous expression of AtNPR1 in oxidative stress tolerance in transgenic tobacco. |
| doi_str_mv | 10.1007/s10529-009-0022-5 |
| format | Article |
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Raja Rajesh ; Meur, G ; Kirti, P. B</creator><creatorcontrib>Srinivasan, T ; Kumar, K. Raja Rajesh ; Meur, G ; Kirti, P. B</creatorcontrib><description>In Arabidopsis, NPR1 (non-expressor of pathogenesis related genes 1, AtNPR1) functions downstream of salicylic acid (SA) and modulates the SA mediated systemic acquired resistance. It is also involved in a cross talk with the jasmonate pathway that is essential for resistance against herbivores and necrotrophic pathogens. Overexpression of AtNPR1 in transgenic plants resulted in enhanced disease resistance. Recently, tobacco transgenic plants expressing AtNPR1 were shown to be tolerant to the early instars of Spodoptera litura (Meur et al., Physiol Plant 133:765-775, 2008). In this communication, we show that the heterologous expression of AtNPR1 in tobacco has also enhanced the oxidative stress tolerance. The transgenic plants exhibited enhanced tolerance to the treatment with methyl viologen. This tolerance was associated with the constitutive upregulation of PR1, PR2 (glucanase), PR5 (thaumatin like protein), ascorbate peroxidase (APX) and Cu²⁺/Zn²⁺ superoxide dismutase (SOD). This is the first demonstration of the novel function of heterologous expression of AtNPR1 in oxidative stress tolerance in transgenic tobacco.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-009-0022-5</identifier><identifier>PMID: 19466562</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Heidelberg: Dordrecht : Springer Netherlands</publisher><subject>Agricultural biotechnology ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis Proteins - biosynthesis ; Arabidopsis Proteins - genetics ; Biological and medical sciences ; Biotechnology ; Disease resistance ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Profiling ; Herbivores ; Nicotiana - genetics ; Nicotiana - physiology ; Oxidative Stress ; Plant Proteins - biosynthesis ; Plant resistance ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - physiology ; Spodoptera litura ; Stress, Physiological ; Tobacco ; Transgenic plants</subject><ispartof>Biotechnology letters, 2009-09, Vol.31 (9), p.1343-1351</ispartof><rights>2009 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-ec76697b28a9aa281c8dc847817df470229681de9588ce467a4cfd40d1352b383</citedby><cites>FETCH-LOGICAL-c442t-ec76697b28a9aa281c8dc847817df470229681de9588ce467a4cfd40d1352b383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,782,786,791,792,23939,23940,25149,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21897357$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19466562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Srinivasan, T</creatorcontrib><creatorcontrib>Kumar, K. Raja Rajesh</creatorcontrib><creatorcontrib>Meur, G</creatorcontrib><creatorcontrib>Kirti, P. B</creatorcontrib><title>Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>In Arabidopsis, NPR1 (non-expressor of pathogenesis related genes 1, AtNPR1) functions downstream of salicylic acid (SA) and modulates the SA mediated systemic acquired resistance. It is also involved in a cross talk with the jasmonate pathway that is essential for resistance against herbivores and necrotrophic pathogens. Overexpression of AtNPR1 in transgenic plants resulted in enhanced disease resistance. Recently, tobacco transgenic plants expressing AtNPR1 were shown to be tolerant to the early instars of Spodoptera litura (Meur et al., Physiol Plant 133:765-775, 2008). In this communication, we show that the heterologous expression of AtNPR1 in tobacco has also enhanced the oxidative stress tolerance. The transgenic plants exhibited enhanced tolerance to the treatment with methyl viologen. This tolerance was associated with the constitutive upregulation of PR1, PR2 (glucanase), PR5 (thaumatin like protein), ascorbate peroxidase (APX) and Cu²⁺/Zn²⁺ superoxide dismutase (SOD). This is the first demonstration of the novel function of heterologous expression of AtNPR1 in oxidative stress tolerance in transgenic tobacco.</description><subject>Agricultural biotechnology</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - biosynthesis</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Disease resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Herbivores</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>Oxidative Stress</subject><subject>Plant Proteins - biosynthesis</subject><subject>Plant resistance</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - physiology</subject><subject>Spodoptera litura</subject><subject>Stress, Physiological</subject><subject>Tobacco</subject><subject>Transgenic plants</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFks2KFDEURoMoTtv6AG40CIouSnPzX8tmUEcYVNRZh1Qq1WaortQkVTK-vQndKLiZRbghOfcjlxOEngJ5C4SodxmIoG1DSF2UNuIe2oBQrJFKyftoQ4BDI3hLz9CjnK9JARVRD9EZtFxKIekGrRd-8SmOcR_XjP3tnHzOIU44DniXbBf6OOeQ8eev3wC_3i21vsF--mkn5zOOt6G3S_jlcV5qJ17i6FO9w2HCS9nlvZ-CK-eddS7iebTTkh-jB4Mds39yqlt09eH9j_OL5vLLx0_nu8vGcU6XxjslZas6qm1rLdXgdO80VxpUP3BVRm6lht63QmvnuVSWu6HnpAcmaMc026JXx9w5xZvV58UcQnZ-LI_wZV4jlRAagN4JMsk4V1LcCVKgQkpSwRf_gddxTVOZtjAgihoKBYIj5FLMOfnBzCkcbPptgJiq2BwVm2LOVMWmBj87Ba_dwff_Ok5OC_DyBNjs7DhUHSH_5SjoVrHyS7bo-ZEbbDR2nwpz9Z0SYASk5AoY-wOYCLa3</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Srinivasan, T</creator><creator>Kumar, K. 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Raja Rajesh ; Meur, G ; Kirti, P. B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-ec76697b28a9aa281c8dc847817df470229681de9588ce467a4cfd40d1352b383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agricultural biotechnology</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - biosynthesis</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Disease resistance</topic><topic>Fundamental and applied biological sciences. 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Raja Rajesh</au><au>Meur, G</au><au>Kirti, P. B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>31</volume><issue>9</issue><spage>1343</spage><epage>1351</epage><pages>1343-1351</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>In Arabidopsis, NPR1 (non-expressor of pathogenesis related genes 1, AtNPR1) functions downstream of salicylic acid (SA) and modulates the SA mediated systemic acquired resistance. It is also involved in a cross talk with the jasmonate pathway that is essential for resistance against herbivores and necrotrophic pathogens. Overexpression of AtNPR1 in transgenic plants resulted in enhanced disease resistance. Recently, tobacco transgenic plants expressing AtNPR1 were shown to be tolerant to the early instars of Spodoptera litura (Meur et al., Physiol Plant 133:765-775, 2008). In this communication, we show that the heterologous expression of AtNPR1 in tobacco has also enhanced the oxidative stress tolerance. The transgenic plants exhibited enhanced tolerance to the treatment with methyl viologen. This tolerance was associated with the constitutive upregulation of PR1, PR2 (glucanase), PR5 (thaumatin like protein), ascorbate peroxidase (APX) and Cu²⁺/Zn²⁺ superoxide dismutase (SOD). This is the first demonstration of the novel function of heterologous expression of AtNPR1 in oxidative stress tolerance in transgenic tobacco.</abstract><cop>Heidelberg</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>19466562</pmid><doi>10.1007/s10529-009-0022-5</doi><tpages>9</tpages></addata></record> |
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| subjects | Agricultural biotechnology Arabidopsis Arabidopsis - genetics Arabidopsis Proteins - biosynthesis Arabidopsis Proteins - genetics Biological and medical sciences Biotechnology Disease resistance Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Herbivores Nicotiana - genetics Nicotiana - physiology Oxidative Stress Plant Proteins - biosynthesis Plant resistance Plants, Genetically Modified - genetics Plants, Genetically Modified - physiology Spodoptera litura Stress, Physiological Tobacco Transgenic plants |
| title | Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants |
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