Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier's disease
The permanent human cell line C3842 was established from a secondary chondrosarcoma in a typical case of Ollier's disease. In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and in...
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| Veröffentlicht in: | Virchows Archiv : an international journal of pathology 2005-03, Vol.446 (3), p.287-299 |
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| creator | KALINSKI, Thomas KRUEGER, Sabine PELZ, Antje-Friederike WIEACKER, Peter HARTIG, Roland RÖPKE, Martin SCHNEIDER-STOCK, Regine DOMBROWSKI, Frank ROESSNER, Albert |
| description | The permanent human cell line C3842 was established from a secondary chondrosarcoma in a typical case of Ollier's disease. In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier's disease in vitro, which is characterized by distinct features of such malignant cartilage tumors. |
| doi_str_mv | 10.1007/s00428-004-1194-y |
| format | Article |
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In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier's disease in vitro, which is characterized by distinct features of such malignant cartilage tumors.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s00428-004-1194-y</identifier><identifier>PMID: 15731924</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adult ; Animals ; Biological and medical sciences ; Bone Neoplasms - complications ; Bone Neoplasms - pathology ; Bone Neoplasms - ultrastructure ; Cell Line, Tumor - physiology ; Cell Line, Tumor - ultrastructure ; Chondrosarcoma - complications ; Chondrosarcoma - pathology ; Chondrosarcoma - ultrastructure ; Chorioallantoic Membrane ; Chromosome Aberrations ; Diseases of the osteoarticular system ; Enchondromatosis - complications ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Neoplasm Invasiveness ; Pathology. 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In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier's disease in vitro, which is characterized by distinct features of such malignant cartilage tumors.</description><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Neoplasms - complications</subject><subject>Bone Neoplasms - pathology</subject><subject>Bone Neoplasms - ultrastructure</subject><subject>Cell Line, Tumor - physiology</subject><subject>Cell Line, Tumor - ultrastructure</subject><subject>Chondrosarcoma - complications</subject><subject>Chondrosarcoma - pathology</subject><subject>Chondrosarcoma - ultrastructure</subject><subject>Chorioallantoic Membrane</subject><subject>Chromosome Aberrations</subject><subject>Diseases of the osteoarticular system</subject><subject>Enchondromatosis - complications</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neoplasm Invasiveness</subject><subject>Pathology. 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In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier's disease in vitro, which is characterized by distinct features of such malignant cartilage tumors.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>15731924</pmid><doi>10.1007/s00428-004-1194-y</doi><tpages>13</tpages></addata></record> |
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| subjects | Adult Animals Biological and medical sciences Bone Neoplasms - complications Bone Neoplasms - pathology Bone Neoplasms - ultrastructure Cell Line, Tumor - physiology Cell Line, Tumor - ultrastructure Chondrosarcoma - complications Chondrosarcoma - pathology Chondrosarcoma - ultrastructure Chorioallantoic Membrane Chromosome Aberrations Diseases of the osteoarticular system Enchondromatosis - complications Humans Immunohistochemistry In Situ Hybridization, Fluorescence Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Neoplasm Invasiveness Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymorphism, Single-Stranded Conformational Reverse Transcriptase Polymerase Chain Reaction Tumors Tumors of striated muscle and skeleton |
| title | Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier's disease |
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