Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle
Nitric oxide (NO) has been proposed as a mediator of postnatal mammary gland development. We investigated the immunohistochemical localization of three isoforms of nitric oxide synthase (NOS) enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS), in the mouse mammary gland du...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2009, Vol.71(7), pp.945-949 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 949 |
|---|---|
| container_issue | 7 |
| container_start_page | 945 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 71 |
| creator | Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture) Matsumoto, M Tsuchida, K Oka, T Kanouchi, H Suzuki, S |
| description | Nitric oxide (NO) has been proposed as a mediator of postnatal mammary gland development. We investigated the immunohistochemical localization of three isoforms of nitric oxide synthase (NOS) enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS), in the mouse mammary gland during reproductive cycle. The NOS isoforms detected in normal mouse mammary glands were the constitutive forms of NOS (nNOS and eNOS). nNOS was localized to the alveoli, myoepithelia, lactiferous ducts and blood vessel endothelia, while eNOS was localized in the alveoli, lactiferous ducts and blood vessel endothelia. The strongest immunoreactivity for both constitutive NOS isoforms was observed in pregnant mice. The differential staining intensity of NOS enzymes in the mammary gland led us to conclude that nitric oxide in the mouse mammary gland is mainly synthesized by constitutive NOS isoforms, and suggest that NO has functional roles in post-pubertal growth and differentiation of the mammary gland. |
| doi_str_mv | 10.1292/jvms.71.945 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67554154</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67554154</sourcerecordid><originalsourceid>FETCH-LOGICAL-c556t-9bf59437d47fae42f54d7474de3881c5e992c9173c84b8191eb9188d032cb6283</originalsourceid><addsrcrecordid>eNpdkV2LEzEUhoMobl298loJCKLI1HxOkjtl8WNlcQX1OmQymTZlktRkZrH-elOmVPDmhJCHhzfvAeApRmtMFHm7uwtlLfBaMX4PrDBlohGMqvtghRRuG0E4ugCPStkhRDBr1UNwgVXLCZN0BfbXIcwxbX2Zkt264K0Z4Zjq9H_M5FOEaYDRT9lbmH773sFyiNPWFAdffb39_hr6CEOa6zWYEEw-wM1oYg_7Ofu4gdntc-pnO_k7B-3Bju4xeDCYsbgnp_MS_Pz44cfV5-bm9tP11fubxnLeTo3qBq4YFT0Tg3GMDJz1ggnWOyolttwpRazCglrJOokVdp3CUvaIEtu1RNJL8HLx1gC_ZlcmHXyxbqzpXM2rW8E5w5xV8MV_4C7NOdZsurYlBZYckUq9WSibUynZDXqf_fG_GiN9XIM-rkELrOsaKv385Jy74Pp_7Kn3CrxbgF2ZzMadAZMnX0s6y8QyqvP8ZLcmaxer4tmiGEzSZpN90V--EYQUQjWzpH8B6lWkew</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468718502</pqid></control><display><type>article</type><title>Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle</title><source>MEDLINE</source><source>EZB Electronic Journals Library</source><source>J-STAGE</source><creator>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture) ; Matsumoto, M ; Tsuchida, K ; Oka, T ; Kanouchi, H ; Suzuki, S</creator><creatorcontrib>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture) ; Matsumoto, M ; Tsuchida, K ; Oka, T ; Kanouchi, H ; Suzuki, S</creatorcontrib><description>Nitric oxide (NO) has been proposed as a mediator of postnatal mammary gland development. We investigated the immunohistochemical localization of three isoforms of nitric oxide synthase (NOS) enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS), in the mouse mammary gland during reproductive cycle. The NOS isoforms detected in normal mouse mammary glands were the constitutive forms of NOS (nNOS and eNOS). nNOS was localized to the alveoli, myoepithelia, lactiferous ducts and blood vessel endothelia, while eNOS was localized in the alveoli, lactiferous ducts and blood vessel endothelia. The strongest immunoreactivity for both constitutive NOS isoforms was observed in pregnant mice. The differential staining intensity of NOS enzymes in the mammary gland led us to conclude that nitric oxide in the mouse mammary gland is mainly synthesized by constitutive NOS isoforms, and suggest that NO has functional roles in post-pubertal growth and differentiation of the mammary gland.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.71.945</identifier><identifier>PMID: 19652483</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>ANIMAL TISSUES ; Animals ; ENZIMAS ; ENZYME ; ENZYMES ; Female ; Gene Expression Regulation, Enzymologic ; GLANDE MAMMAIRE ; GLANDULAS MAMARIAS ; Immunohistochemistry ; IMMUNOLOGICAL TECHNIQUES ; mammary gland ; MAMMARY GLANDS ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - metabolism ; MICE ; mouse ; NITRIC OXIDE ; Nitric Oxide Synthase - genetics ; Nitric Oxide Synthase - metabolism ; NOS ; OXIDO NITRICO ; OXYDE NITRIQUE ; PERINATAL PERIOD ; PERIODE PERINATALE ; PERIODO PERINATAL ; Pregnancy ; Protein Isoforms ; Protein Transport ; RATON ; REPRODUCCION ; REPRODUCTION ; reproductive cycle ; SOURIS ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS ; TEJIDOS ANIMALES ; TISSU ANIMAL</subject><ispartof>Journal of Veterinary Medical Science, 2009, Vol.71(7), pp.945-949</ispartof><rights>2009 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-9bf59437d47fae42f54d7474de3881c5e992c9173c84b8191eb9188d032cb6283</citedby><cites>FETCH-LOGICAL-c556t-9bf59437d47fae42f54d7474de3881c5e992c9173c84b8191eb9188d032cb6283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19652483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Matsumoto, M</creatorcontrib><creatorcontrib>Tsuchida, K</creatorcontrib><creatorcontrib>Oka, T</creatorcontrib><creatorcontrib>Kanouchi, H</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><title>Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Nitric oxide (NO) has been proposed as a mediator of postnatal mammary gland development. We investigated the immunohistochemical localization of three isoforms of nitric oxide synthase (NOS) enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS), in the mouse mammary gland during reproductive cycle. The NOS isoforms detected in normal mouse mammary glands were the constitutive forms of NOS (nNOS and eNOS). nNOS was localized to the alveoli, myoepithelia, lactiferous ducts and blood vessel endothelia, while eNOS was localized in the alveoli, lactiferous ducts and blood vessel endothelia. The strongest immunoreactivity for both constitutive NOS isoforms was observed in pregnant mice. The differential staining intensity of NOS enzymes in the mammary gland led us to conclude that nitric oxide in the mouse mammary gland is mainly synthesized by constitutive NOS isoforms, and suggest that NO has functional roles in post-pubertal growth and differentiation of the mammary gland.</description><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>ENZIMAS</subject><subject>ENZYME</subject><subject>ENZYMES</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>GLANDE MAMMAIRE</subject><subject>GLANDULAS MAMARIAS</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>mammary gland</subject><subject>MAMMARY GLANDS</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>MICE</subject><subject>mouse</subject><subject>NITRIC OXIDE</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>NOS</subject><subject>OXIDO NITRICO</subject><subject>OXYDE NITRIQUE</subject><subject>PERINATAL PERIOD</subject><subject>PERIODE PERINATALE</subject><subject>PERIODO PERINATAL</subject><subject>Pregnancy</subject><subject>Protein Isoforms</subject><subject>Protein Transport</subject><subject>RATON</subject><subject>REPRODUCCION</subject><subject>REPRODUCTION</subject><subject>reproductive cycle</subject><subject>SOURIS</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><subject>TEJIDOS ANIMALES</subject><subject>TISSU ANIMAL</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV2LEzEUhoMobl298loJCKLI1HxOkjtl8WNlcQX1OmQymTZlktRkZrH-elOmVPDmhJCHhzfvAeApRmtMFHm7uwtlLfBaMX4PrDBlohGMqvtghRRuG0E4ugCPStkhRDBr1UNwgVXLCZN0BfbXIcwxbX2Zkt264K0Z4Zjq9H_M5FOEaYDRT9lbmH773sFyiNPWFAdffb39_hr6CEOa6zWYEEw-wM1oYg_7Ofu4gdntc-pnO_k7B-3Bju4xeDCYsbgnp_MS_Pz44cfV5-bm9tP11fubxnLeTo3qBq4YFT0Tg3GMDJz1ggnWOyolttwpRazCglrJOokVdp3CUvaIEtu1RNJL8HLx1gC_ZlcmHXyxbqzpXM2rW8E5w5xV8MV_4C7NOdZsurYlBZYckUq9WSibUynZDXqf_fG_GiN9XIM-rkELrOsaKv385Jy74Pp_7Kn3CrxbgF2ZzMadAZMnX0s6y8QyqvP8ZLcmaxer4tmiGEzSZpN90V--EYQUQjWzpH8B6lWkew</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture)</creator><creator>Matsumoto, M</creator><creator>Tsuchida, K</creator><creator>Oka, T</creator><creator>Kanouchi, H</creator><creator>Suzuki, S</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle</title><author>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture) ; Matsumoto, M ; Tsuchida, K ; Oka, T ; Kanouchi, H ; Suzuki, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-9bf59437d47fae42f54d7474de3881c5e992c9173c84b8191eb9188d032cb6283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ANIMAL TISSUES</topic><topic>Animals</topic><topic>ENZIMAS</topic><topic>ENZYME</topic><topic>ENZYMES</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>GLANDE MAMMAIRE</topic><topic>GLANDULAS MAMARIAS</topic><topic>Immunohistochemistry</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>mammary gland</topic><topic>MAMMARY GLANDS</topic><topic>Mammary Glands, Animal - cytology</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>MICE</topic><topic>mouse</topic><topic>NITRIC OXIDE</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>NOS</topic><topic>OXIDO NITRICO</topic><topic>OXYDE NITRIQUE</topic><topic>PERINATAL PERIOD</topic><topic>PERIODE PERINATALE</topic><topic>PERIODO PERINATAL</topic><topic>Pregnancy</topic><topic>Protein Isoforms</topic><topic>Protein Transport</topic><topic>RATON</topic><topic>REPRODUCCION</topic><topic>REPRODUCTION</topic><topic>reproductive cycle</topic><topic>SOURIS</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><topic>TEJIDOS ANIMALES</topic><topic>TISSU ANIMAL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Matsumoto, M</creatorcontrib><creatorcontrib>Tsuchida, K</creatorcontrib><creatorcontrib>Oka, T</creatorcontrib><creatorcontrib>Kanouchi, H</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Islam, M.S.(Kagoshima Univ. (Japan). Faculty of Agriculture)</au><au>Matsumoto, M</au><au>Tsuchida, K</au><au>Oka, T</au><au>Kanouchi, H</au><au>Suzuki, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>71</volume><issue>7</issue><spage>945</spage><epage>949</epage><pages>945-949</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Nitric oxide (NO) has been proposed as a mediator of postnatal mammary gland development. We investigated the immunohistochemical localization of three isoforms of nitric oxide synthase (NOS) enzymes, neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS), in the mouse mammary gland during reproductive cycle. The NOS isoforms detected in normal mouse mammary glands were the constitutive forms of NOS (nNOS and eNOS). nNOS was localized to the alveoli, myoepithelia, lactiferous ducts and blood vessel endothelia, while eNOS was localized in the alveoli, lactiferous ducts and blood vessel endothelia. The strongest immunoreactivity for both constitutive NOS isoforms was observed in pregnant mice. The differential staining intensity of NOS enzymes in the mammary gland led us to conclude that nitric oxide in the mouse mammary gland is mainly synthesized by constitutive NOS isoforms, and suggest that NO has functional roles in post-pubertal growth and differentiation of the mammary gland.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>19652483</pmid><doi>10.1292/jvms.71.945</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2009, Vol.71(7), pp.945-949 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67554154 |
| source | MEDLINE; EZB Electronic Journals Library; J-STAGE |
| subjects | ANIMAL TISSUES Animals ENZIMAS ENZYME ENZYMES Female Gene Expression Regulation, Enzymologic GLANDE MAMMAIRE GLANDULAS MAMARIAS Immunohistochemistry IMMUNOLOGICAL TECHNIQUES mammary gland MAMMARY GLANDS Mammary Glands, Animal - cytology Mammary Glands, Animal - metabolism MICE mouse NITRIC OXIDE Nitric Oxide Synthase - genetics Nitric Oxide Synthase - metabolism NOS OXIDO NITRICO OXYDE NITRIQUE PERINATAL PERIOD PERIODE PERINATALE PERIODO PERINATAL Pregnancy Protein Isoforms Protein Transport RATON REPRODUCCION REPRODUCTION reproductive cycle SOURIS TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS TEJIDOS ANIMALES TISSU ANIMAL |
| title | Immunohistochemical localization of nitric oxide synthase (NOS) in mouse mammary gland during reproductive cycle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T17%3A18%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunohistochemical%20localization%20of%20nitric%20oxide%20synthase%20(NOS)%20in%20mouse%20mammary%20gland%20during%20reproductive%20cycle&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=Islam,%20M.S.(Kagoshima%20Univ.%20(Japan).%20Faculty%20of%20Agriculture)&rft.date=2009-07-01&rft.volume=71&rft.issue=7&rft.spage=945&rft.epage=949&rft.pages=945-949&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.71.945&rft_dat=%3Cproquest_cross%3E67554154%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468718502&rft_id=info:pmid/19652483&rfr_iscdi=true |