Regulation and Signal Transduction of Toll-Like Receptors in Human Chorioncarcinoma Cell Lines
Problem: Toll‐like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement. Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase cha...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2005-02, Vol.53 (2), p.77-84 |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Klaffenbach, Daniela Rascher, Wolfgang Röllinghoff, Martin Dötsch, Jörg Meißner, Udo Schnare, Markus |
| description | Problem: Toll‐like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement.
Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence‐activated cell sorter analysis. Activation of NF‐κB was determined in a reporter assay system and the activation of the mitogen‐activated protein kinase signaling pathways by immunoblot analysis.
Results: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF‐κB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin‐8.
Conclusion: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus. |
| doi_str_mv | 10.1111/j.1600-0897.2004.00247.x |
| format | Article |
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Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence‐activated cell sorter analysis. Activation of NF‐κB was determined in a reporter assay system and the activation of the mitogen‐activated protein kinase signaling pathways by immunoblot analysis.
Results: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF‐κB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin‐8.
Conclusion: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2004.00247.x</identifier><identifier>PMID: 15790341</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cell Line, Tumor ; Choriocarcinoma - genetics ; Choriocarcinoma - immunology ; Female ; Gene Expression Regulation ; Humans ; Innate immune response ; Interleukin-8 - immunology ; Lipopolysaccharides - pharmacology ; Membrane Glycoproteins - drug effects ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - immunology ; mitogen-activated protein kinase ; Mitogen-Activated Protein Kinases - metabolism ; NF-kappa B - metabolism ; NF-κB ; Oligonucleotides - pharmacology ; placenta ; Placenta - cytology ; Placenta - immunology ; Pregnancy ; Receptors, Cell Surface - drug effects ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - drug effects ; RNA, Messenger - genetics ; Signal Transduction - immunology ; Toll-Like Receptor 2 ; Toll-Like Receptors ; Trophoblasts - immunology</subject><ispartof>American journal of reproductive immunology (1989), 2005-02, Vol.53 (2), p.77-84</ispartof><rights>(c) Blackwell Munksgaard, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4707-7442caee9f3395ba81a64695a35b4b5b377febe70beb02d3d49ae79c36eb219f3</citedby><cites>FETCH-LOGICAL-c4707-7442caee9f3395ba81a64695a35b4b5b377febe70beb02d3d49ae79c36eb219f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.2004.00247.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.2004.00247.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15790341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klaffenbach, Daniela</creatorcontrib><creatorcontrib>Rascher, Wolfgang</creatorcontrib><creatorcontrib>Röllinghoff, Martin</creatorcontrib><creatorcontrib>Dötsch, Jörg</creatorcontrib><creatorcontrib>Meißner, Udo</creatorcontrib><creatorcontrib>Schnare, Markus</creatorcontrib><title>Regulation and Signal Transduction of Toll-Like Receptors in Human Chorioncarcinoma Cell Lines</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem: Toll‐like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement.
Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence‐activated cell sorter analysis. Activation of NF‐κB was determined in a reporter assay system and the activation of the mitogen‐activated protein kinase signaling pathways by immunoblot analysis.
Results: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF‐κB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin‐8.
Conclusion: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.</description><subject>Cell Line, Tumor</subject><subject>Choriocarcinoma - genetics</subject><subject>Choriocarcinoma - immunology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Innate immune response</subject><subject>Interleukin-8 - immunology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Membrane Glycoproteins - drug effects</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - immunology</subject><subject>mitogen-activated protein kinase</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>NF-kappa B - metabolism</subject><subject>NF-κB</subject><subject>Oligonucleotides - pharmacology</subject><subject>placenta</subject><subject>Placenta - cytology</subject><subject>Placenta - immunology</subject><subject>Pregnancy</subject><subject>Receptors, Cell Surface - drug effects</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>Signal Transduction - immunology</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-Like Receptors</subject><subject>Trophoblasts - immunology</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFP2zAUha0JNBjwFyY_8ZbMiZ04kXhBFbRU0ZBoJ3jCsp0b5pLYxW608u_n0oq9zi--uj7fvccHIZyRNIvnxyrNSkISUtU8zQlhKSE54-n2Czr9fDiKNWFlwhmpTtC3EFaExD7lX9FJVvCaUJadoucHeBl7uTHOYmlbvDAvVvZ46aUN7ag_-q7DS9f3SWNeAT-AhvXG-YCNxbNxkBZPfjsfdVp6bawbJJ5A3-PGWAjn6LiTfYCLw32Gft3eLCezpLmf3k2um0QzTnj0yHItAeqO0rpQsspkycq6kLRQTBWKct6BAk4UKJK3tGW1BF5rWoLKs0idocv93LV3byOEjRhM0NGGtODGIEpeFPG_VRRWe6H2LgQPnVh7M0j_LjIidtmKldhFKHYRil224iNbsY3o98OOUQ3Q_gMPYUbB1V7wx_Tw_t-DxfX8LhYRT_a4CRvYfuLSv0b7lBfi8edUNIvHxfxpNhdT-heAxJen</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Klaffenbach, Daniela</creator><creator>Rascher, Wolfgang</creator><creator>Röllinghoff, Martin</creator><creator>Dötsch, Jörg</creator><creator>Meißner, Udo</creator><creator>Schnare, Markus</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Regulation and Signal Transduction of Toll-Like Receptors in Human Chorioncarcinoma Cell Lines</title><author>Klaffenbach, Daniela ; Rascher, Wolfgang ; Röllinghoff, Martin ; Dötsch, Jörg ; Meißner, Udo ; Schnare, Markus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4707-7442caee9f3395ba81a64695a35b4b5b377febe70beb02d3d49ae79c36eb219f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Cell Line, Tumor</topic><topic>Choriocarcinoma - genetics</topic><topic>Choriocarcinoma - immunology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Innate immune response</topic><topic>Interleukin-8 - immunology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Membrane Glycoproteins - drug effects</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - immunology</topic><topic>mitogen-activated protein kinase</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>NF-kappa B - metabolism</topic><topic>NF-κB</topic><topic>Oligonucleotides - pharmacology</topic><topic>placenta</topic><topic>Placenta - cytology</topic><topic>Placenta - immunology</topic><topic>Pregnancy</topic><topic>Receptors, Cell Surface - drug effects</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction - immunology</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-Like Receptors</topic><topic>Trophoblasts - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klaffenbach, Daniela</creatorcontrib><creatorcontrib>Rascher, Wolfgang</creatorcontrib><creatorcontrib>Röllinghoff, Martin</creatorcontrib><creatorcontrib>Dötsch, Jörg</creatorcontrib><creatorcontrib>Meißner, Udo</creatorcontrib><creatorcontrib>Schnare, Markus</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klaffenbach, Daniela</au><au>Rascher, Wolfgang</au><au>Röllinghoff, Martin</au><au>Dötsch, Jörg</au><au>Meißner, Udo</au><au>Schnare, Markus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation and Signal Transduction of Toll-Like Receptors in Human Chorioncarcinoma Cell Lines</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2005-02</date><risdate>2005</risdate><volume>53</volume><issue>2</issue><spage>77</spage><epage>84</epage><pages>77-84</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem: Toll‐like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement.
Methods of study: In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence‐activated cell sorter analysis. Activation of NF‐κB was determined in a reporter assay system and the activation of the mitogen‐activated protein kinase signaling pathways by immunoblot analysis.
Results: Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF‐κB, as well as the phosphorylation of ERK1/2 and p38 MAP kinases and secretion of interleukin‐8.
Conclusion: The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15790341</pmid><doi>10.1111/j.1600-0897.2004.00247.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 2005-02, Vol.53 (2), p.77-84 |
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| subjects | Cell Line, Tumor Choriocarcinoma - genetics Choriocarcinoma - immunology Female Gene Expression Regulation Humans Innate immune response Interleukin-8 - immunology Lipopolysaccharides - pharmacology Membrane Glycoproteins - drug effects Membrane Glycoproteins - genetics Membrane Glycoproteins - immunology mitogen-activated protein kinase Mitogen-Activated Protein Kinases - metabolism NF-kappa B - metabolism NF-κB Oligonucleotides - pharmacology placenta Placenta - cytology Placenta - immunology Pregnancy Receptors, Cell Surface - drug effects Receptors, Cell Surface - genetics Receptors, Cell Surface - immunology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - drug effects RNA, Messenger - genetics Signal Transduction - immunology Toll-Like Receptor 2 Toll-Like Receptors Trophoblasts - immunology |
| title | Regulation and Signal Transduction of Toll-Like Receptors in Human Chorioncarcinoma Cell Lines |
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