Heterogeneity in distribution of amyloid-positive islets in type-2 diabetic patients
Amyloid-containing (A+) islets are characteristic for type-2 diabetes (T2D), but their abundance seems variable among patients. It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islet...
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| Veröffentlicht in: | Virchows Archiv : an international journal of pathology 2005-03, Vol.446 (3), p.232-238 |
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| container_title | Virchows Archiv : an international journal of pathology |
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| creator | BORROMEO, Cecilia M POTTIER, Xavier IN'T VELD, Peter A PIPELEERS-MARICHAL, Miriam A KAUFMAN, Leonard PIPELEERS, Daniel G VAN SCHRAVENDIJK, Christiaan F |
| description | Amyloid-containing (A+) islets are characteristic for type-2 diabetes (T2D), but their abundance seems variable among patients. It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islets in eight pancreata of T2D patients and eight sex- and age-matched non-diabetic subjects. Transversal sections of head, body and tail segments were stained with synaptophysin combined with Congo red to map/quantify islet tissue and amyloid. In the eight T2D pancreata, the overall percentage of A+ islets varied from 4% to 85%. Further analysis in body and tail indicated that peripheral regions exhibited higher percentages of A+ islets than central regions (averages of, respectively, 30% and 17%, P |
| doi_str_mv | 10.1007/s00428-004-1171-5 |
| format | Article |
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It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islets in eight pancreata of T2D patients and eight sex- and age-matched non-diabetic subjects. Transversal sections of head, body and tail segments were stained with synaptophysin combined with Congo red to map/quantify islet tissue and amyloid. In the eight T2D pancreata, the overall percentage of A+ islets varied from 4% to 85%. Further analysis in body and tail indicated that peripheral regions exhibited higher percentages of A+ islets than central regions (averages of, respectively, 30% and 17%, P<0.05). Non-diabetic control pancreata also exhibited A+ islets, albeit at a 25-fold lower frequency; a tendency towards higher percentage of A+ islets in peripheral versus central regions was also observed. The higher percentage A+ islets in peripheral regions was associated with a higher density and relative islet over exocrine surface area. These observations on heterogeneity in abundance and distribution of A+ islets need consideration when sampling tissue for studies on human islet amyloidosis. The present methodology allows us to further investigate the susceptibility to amyloidosis of islets in peripheral regions of the pancreas.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s00428-004-1171-5</identifier><identifier>PMID: 15791482</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Aged ; Aged, 80 and over ; Amyloid - metabolism ; Biological and medical sciences ; Diabetes Mellitus, Type 2 - metabolism ; Diabetes Mellitus, Type 2 - pathology ; Female ; Heterogeneity ; Humans ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Islets of Langerhans - metabolism ; Islets of Langerhans - pathology ; Male ; Medical sciences ; Pancreas ; Pancreatic Polypeptide - metabolism ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Regions</subject><ispartof>Virchows Archiv : an international journal of pathology, 2005-03, Vol.446 (3), p.232-238</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-4d0e24d2da62e19859ef5cefa9b229d9ef073d6a3fc2a9e8f9df4f005ad910c33</citedby><cites>FETCH-LOGICAL-c356t-4d0e24d2da62e19859ef5cefa9b229d9ef073d6a3fc2a9e8f9df4f005ad910c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16661497$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15791482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BORROMEO, Cecilia M</creatorcontrib><creatorcontrib>POTTIER, Xavier</creatorcontrib><creatorcontrib>IN'T VELD, Peter A</creatorcontrib><creatorcontrib>PIPELEERS-MARICHAL, Miriam A</creatorcontrib><creatorcontrib>KAUFMAN, Leonard</creatorcontrib><creatorcontrib>PIPELEERS, Daniel G</creatorcontrib><creatorcontrib>VAN SCHRAVENDIJK, Christiaan F</creatorcontrib><title>Heterogeneity in distribution of amyloid-positive islets in type-2 diabetic patients</title><title>Virchows Archiv : an international journal of pathology</title><addtitle>Virchows Arch</addtitle><description>Amyloid-containing (A+) islets are characteristic for type-2 diabetes (T2D), but their abundance seems variable among patients. It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islets in eight pancreata of T2D patients and eight sex- and age-matched non-diabetic subjects. Transversal sections of head, body and tail segments were stained with synaptophysin combined with Congo red to map/quantify islet tissue and amyloid. In the eight T2D pancreata, the overall percentage of A+ islets varied from 4% to 85%. Further analysis in body and tail indicated that peripheral regions exhibited higher percentages of A+ islets than central regions (averages of, respectively, 30% and 17%, P<0.05). Non-diabetic control pancreata also exhibited A+ islets, albeit at a 25-fold lower frequency; a tendency towards higher percentage of A+ islets in peripheral versus central regions was also observed. The higher percentage A+ islets in peripheral regions was associated with a higher density and relative islet over exocrine surface area. These observations on heterogeneity in abundance and distribution of A+ islets need consideration when sampling tissue for studies on human islet amyloidosis. The present methodology allows us to further investigate the susceptibility to amyloidosis of islets in peripheral regions of the pancreas.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amyloid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Diabetes Mellitus, Type 2 - pathology</subject><subject>Female</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Islets of Langerhans - metabolism</subject><subject>Islets of Langerhans - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pancreas</subject><subject>Pancreatic Polypeptide - metabolism</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Miscellaneous investigative techniques</topic><topic>Regions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BORROMEO, Cecilia M</creatorcontrib><creatorcontrib>POTTIER, Xavier</creatorcontrib><creatorcontrib>IN'T VELD, Peter A</creatorcontrib><creatorcontrib>PIPELEERS-MARICHAL, Miriam A</creatorcontrib><creatorcontrib>KAUFMAN, Leonard</creatorcontrib><creatorcontrib>PIPELEERS, Daniel G</creatorcontrib><creatorcontrib>VAN SCHRAVENDIJK, Christiaan F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Virchows Archiv : an international journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BORROMEO, Cecilia M</au><au>POTTIER, Xavier</au><au>IN'T VELD, Peter A</au><au>PIPELEERS-MARICHAL, Miriam A</au><au>KAUFMAN, Leonard</au><au>PIPELEERS, Daniel G</au><au>VAN SCHRAVENDIJK, Christiaan F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterogeneity in distribution of amyloid-positive islets in type-2 diabetic patients</atitle><jtitle>Virchows Archiv : an international journal of pathology</jtitle><addtitle>Virchows Arch</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>446</volume><issue>3</issue><spage>232</spage><epage>238</epage><pages>232-238</pages><issn>0945-6317</issn><eissn>1432-2307</eissn><abstract>Amyloid-containing (A+) islets are characteristic for type-2 diabetes (T2D), but their abundance seems variable among patients. It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islets in eight pancreata of T2D patients and eight sex- and age-matched non-diabetic subjects. Transversal sections of head, body and tail segments were stained with synaptophysin combined with Congo red to map/quantify islet tissue and amyloid. In the eight T2D pancreata, the overall percentage of A+ islets varied from 4% to 85%. Further analysis in body and tail indicated that peripheral regions exhibited higher percentages of A+ islets than central regions (averages of, respectively, 30% and 17%, P<0.05). Non-diabetic control pancreata also exhibited A+ islets, albeit at a 25-fold lower frequency; a tendency towards higher percentage of A+ islets in peripheral versus central regions was also observed. The higher percentage A+ islets in peripheral regions was associated with a higher density and relative islet over exocrine surface area. These observations on heterogeneity in abundance and distribution of A+ islets need consideration when sampling tissue for studies on human islet amyloidosis. The present methodology allows us to further investigate the susceptibility to amyloidosis of islets in peripheral regions of the pancreas.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>15791482</pmid><doi>10.1007/s00428-004-1171-5</doi><tpages>7</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Amyloid - metabolism Biological and medical sciences Diabetes Mellitus, Type 2 - metabolism Diabetes Mellitus, Type 2 - pathology Female Heterogeneity Humans Image Processing, Computer-Assisted Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Islets of Langerhans - metabolism Islets of Langerhans - pathology Male Medical sciences Pancreas Pancreatic Polypeptide - metabolism Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Regions |
| title | Heterogeneity in distribution of amyloid-positive islets in type-2 diabetic patients |
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