Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro
Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointe...
Gespeichert in:
| Veröffentlicht in: | Molecular nutrition & food research 2009-08, Vol.53 (8), p.1007-1018 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1018 |
|---|---|
| container_issue | 8 |
| container_start_page | 1007 |
| container_title | Molecular nutrition & food research |
| container_volume | 53 |
| creator | Martinez-Villaluenga, Cristina Dia, Vermont P Berhow, Mark Bringe, Neal A Gonzalez de Mejia, Elvira |
| description | Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β-conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E₂ (PGE₂) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/NO and COX-2/PGE₂ pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food. |
| doi_str_mv | 10.1002/mnfr.200800473 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67551559</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67551559</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4353-d22f4f7d464a1ba93097642b4e1029f7fc1aee25ada71df4b42a2b65439342fc3</originalsourceid><addsrcrecordid>eNqFkMtu1DAUhiMEoqWwZQnZwC6Dr8lkiSo6XEqpoBVL68SXGYNjT-2kbV6LB-GZ8CijgR0rH_l8_3_O-YviOUYLjBB503sTFwShJUKsoQ-KY1xjWjFM6cNDTfhR8SSlHwhRTBh9XBzhtkaUIXZc3F_GMGjry82kYnBTgkGn0sTQl79_VTL4tZuk9RnQPlq50apMYeo0-HKtfRimbcat39jODqWzW6tKkHLsRweDDb4Er3LbOOj7-SM73dohhqfFIwMu6Wf796S4Pnt3dfq-Ov-y-nD69rySjHJaKUIMM41iNQPcQUtR29SMdExjRFrTGIlBa8JBQYOVYR0jQLqaM9pSRoykJ8Xr2Xcbw82o0yB6m6R2DrwOYxJ1wznmvM3gYgZlDClFbcQ22h7iJDASu6zFLmtxyDoLXuydx67X6i--DzcDr_YAJAnORPDSpgNH8JIw1O4mtzN3Z52e_jNWfL44-_rvEtWstWnQ9wctxJ_5Mtpw8f1iJS45XX5afbwSOPMvZ95AELCOeZ_rbwRhinBdc0xa-gd6Y7Ql</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67551559</pqid></control><display><type>article</type><title>Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Martinez-Villaluenga, Cristina ; Dia, Vermont P ; Berhow, Mark ; Bringe, Neal A ; Gonzalez de Mejia, Elvira</creator><creatorcontrib>Martinez-Villaluenga, Cristina ; Dia, Vermont P ; Berhow, Mark ; Bringe, Neal A ; Gonzalez de Mejia, Elvira</creatorcontrib><description>Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β-conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E₂ (PGE₂) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/NO and COX-2/PGE₂ pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.</description><identifier>ISSN: 1613-4125</identifier><identifier>EISSN: 1613-4133</identifier><identifier>DOI: 10.1002/mnfr.200800473</identifier><identifier>PMID: 19603404</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>3T3-L1 Cells ; Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts ; Animals ; Antigens, Plant - pharmacology ; Biological and medical sciences ; Biomarkers ; Cyclooxygenase 2 Inhibitors - pharmacology ; Dinoprostone - biosynthesis ; Fatty Acid Synthases - genetics ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; Genotype ; Globulins - pharmacology ; Glycine max - chemistry ; Inflammation ; Inflammation - prevention & control ; Lipid metabolism ; Lipid Metabolism - drug effects ; Lipoprotein Lipase - genetics ; Mice ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase Type II - antagonists & inhibitors ; Plant Proteins - analysis ; Seed Storage Proteins - pharmacology ; Soy protein hydrolysates ; Soybean Proteins - pharmacology ; β-conglycinin</subject><ispartof>Molecular nutrition & food research, 2009-08, Vol.53 (8), p.1007-1018</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4353-d22f4f7d464a1ba93097642b4e1029f7fc1aee25ada71df4b42a2b65439342fc3</citedby><cites>FETCH-LOGICAL-c4353-d22f4f7d464a1ba93097642b4e1029f7fc1aee25ada71df4b42a2b65439342fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmnfr.200800473$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmnfr.200800473$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21824099$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19603404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Villaluenga, Cristina</creatorcontrib><creatorcontrib>Dia, Vermont P</creatorcontrib><creatorcontrib>Berhow, Mark</creatorcontrib><creatorcontrib>Bringe, Neal A</creatorcontrib><creatorcontrib>Gonzalez de Mejia, Elvira</creatorcontrib><title>Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro</title><title>Molecular nutrition & food research</title><addtitle>Mol. Nutr. Food Res</addtitle><description>Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β-conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E₂ (PGE₂) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/NO and COX-2/PGE₂ pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.</description><subject>3T3-L1 Cells</subject><subject>Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts</subject><subject>Animals</subject><subject>Antigens, Plant - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Cyclooxygenase 2 Inhibitors - pharmacology</subject><subject>Dinoprostone - biosynthesis</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Globulins - pharmacology</subject><subject>Glycine max - chemistry</subject><subject>Inflammation</subject><subject>Inflammation - prevention & control</subject><subject>Lipid metabolism</subject><subject>Lipid Metabolism - drug effects</subject><subject>Lipoprotein Lipase - genetics</subject><subject>Mice</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase Type II - antagonists & inhibitors</subject><subject>Plant Proteins - analysis</subject><subject>Seed Storage Proteins - pharmacology</subject><subject>Soy protein hydrolysates</subject><subject>Soybean Proteins - pharmacology</subject><subject>β-conglycinin</subject><issn>1613-4125</issn><issn>1613-4133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu1DAUhiMEoqWwZQnZwC6Dr8lkiSo6XEqpoBVL68SXGYNjT-2kbV6LB-GZ8CijgR0rH_l8_3_O-YviOUYLjBB503sTFwShJUKsoQ-KY1xjWjFM6cNDTfhR8SSlHwhRTBh9XBzhtkaUIXZc3F_GMGjry82kYnBTgkGn0sTQl79_VTL4tZuk9RnQPlq50apMYeo0-HKtfRimbcat39jODqWzW6tKkHLsRweDDb4Er3LbOOj7-SM73dohhqfFIwMu6Wf796S4Pnt3dfq-Ov-y-nD69rySjHJaKUIMM41iNQPcQUtR29SMdExjRFrTGIlBa8JBQYOVYR0jQLqaM9pSRoykJ8Xr2Xcbw82o0yB6m6R2DrwOYxJ1wznmvM3gYgZlDClFbcQ22h7iJDASu6zFLmtxyDoLXuydx67X6i--DzcDr_YAJAnORPDSpgNH8JIw1O4mtzN3Z52e_jNWfL44-_rvEtWstWnQ9wctxJ_5Mtpw8f1iJS45XX5afbwSOPMvZ95AELCOeZ_rbwRhinBdc0xa-gd6Y7Ql</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Martinez-Villaluenga, Cristina</creator><creator>Dia, Vermont P</creator><creator>Berhow, Mark</creator><creator>Bringe, Neal A</creator><creator>Gonzalez de Mejia, Elvira</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro</title><author>Martinez-Villaluenga, Cristina ; Dia, Vermont P ; Berhow, Mark ; Bringe, Neal A ; Gonzalez de Mejia, Elvira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4353-d22f4f7d464a1ba93097642b4e1029f7fc1aee25ada71df4b42a2b65439342fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>3T3-L1 Cells</topic><topic>Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts</topic><topic>Animals</topic><topic>Antigens, Plant - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Cyclooxygenase 2 Inhibitors - pharmacology</topic><topic>Dinoprostone - biosynthesis</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Globulins - pharmacology</topic><topic>Glycine max - chemistry</topic><topic>Inflammation</topic><topic>Inflammation - prevention & control</topic><topic>Lipid metabolism</topic><topic>Lipid Metabolism - drug effects</topic><topic>Lipoprotein Lipase - genetics</topic><topic>Mice</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase Type II - antagonists & inhibitors</topic><topic>Plant Proteins - analysis</topic><topic>Seed Storage Proteins - pharmacology</topic><topic>Soy protein hydrolysates</topic><topic>Soybean Proteins - pharmacology</topic><topic>β-conglycinin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Villaluenga, Cristina</creatorcontrib><creatorcontrib>Dia, Vermont P</creatorcontrib><creatorcontrib>Berhow, Mark</creatorcontrib><creatorcontrib>Bringe, Neal A</creatorcontrib><creatorcontrib>Gonzalez de Mejia, Elvira</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular nutrition & food research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez-Villaluenga, Cristina</au><au>Dia, Vermont P</au><au>Berhow, Mark</au><au>Bringe, Neal A</au><au>Gonzalez de Mejia, Elvira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro</atitle><jtitle>Molecular nutrition & food research</jtitle><addtitle>Mol. Nutr. Food Res</addtitle><date>2009-08</date><risdate>2009</risdate><volume>53</volume><issue>8</issue><spage>1007</spage><epage>1018</epage><pages>1007-1018</pages><issn>1613-4125</issn><eissn>1613-4133</eissn><abstract>Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β-conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E₂ (PGE₂) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/NO and COX-2/PGE₂ pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>19603404</pmid><doi>10.1002/mnfr.200800473</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1613-4125 |
| ispartof | Molecular nutrition & food research, 2009-08, Vol.53 (8), p.1007-1018 |
| issn | 1613-4125 1613-4133 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67551559 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | 3T3-L1 Cells Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts Animals Antigens, Plant - pharmacology Biological and medical sciences Biomarkers Cyclooxygenase 2 Inhibitors - pharmacology Dinoprostone - biosynthesis Fatty Acid Synthases - genetics Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Genotype Globulins - pharmacology Glycine max - chemistry Inflammation Inflammation - prevention & control Lipid metabolism Lipid Metabolism - drug effects Lipoprotein Lipase - genetics Mice Nitric Oxide - biosynthesis Nitric Oxide Synthase Type II - antagonists & inhibitors Plant Proteins - analysis Seed Storage Proteins - pharmacology Soy protein hydrolysates Soybean Proteins - pharmacology β-conglycinin |
| title | Protein hydrolysates from β-conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T20%3A55%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein%20hydrolysates%20from%20%CE%B2-conglycinin%20enriched%20soybean%20genotypes%20inhibit%20lipid%20accumulation%20and%20inflammation%20in%20vitro&rft.jtitle=Molecular%20nutrition%20&%20food%20research&rft.au=Martinez-Villaluenga,%20Cristina&rft.date=2009-08&rft.volume=53&rft.issue=8&rft.spage=1007&rft.epage=1018&rft.pages=1007-1018&rft.issn=1613-4125&rft.eissn=1613-4133&rft_id=info:doi/10.1002/mnfr.200800473&rft_dat=%3Cproquest_cross%3E67551559%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67551559&rft_id=info:pmid/19603404&rfr_iscdi=true |