DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality
Objective To determine the meiotic segregation and DNA fragmentation in spermatozoa of carriers of a chromosomal structural abnormality. Design Case series. Setting University hospital. Patient(s) Thirty-seven male carriers of a chromosomal structural abnormality (21 with a balanced reciprocal trans...
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| Veröffentlicht in: | Fertility and sterility 2009-08, Vol.92 (2), p.583-589 |
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| creator | Perrin, Aurore, M.Sc Caer, Emilie, M.D Oliver-Bonet, Maria, M.D Navarro, Joaquima, M.D Benet, Jordi, M.D Amice, Véronique, M.D De Braekeleer, Marc, M.D., Ph.D Morel, Frédéric, Ph.D |
| description | Objective To determine the meiotic segregation and DNA fragmentation in spermatozoa of carriers of a chromosomal structural abnormality. Design Case series. Setting University hospital. Patient(s) Thirty-seven male carriers of a chromosomal structural abnormality (21 with a balanced reciprocal translocation, 7 with a robertsonian translocation, 9 with a pericentric inversion). Intervention(s) Meiotic segregation was analyzed by the human sperm–hamster oocyte fusion technique or by fluorescent in situ hybridization, and DNA fragmentation was detected by terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling assay. Main Outcome Measure(s) Relationships between abnormal sperm parameters, DNA fragmentation, and meiotic mechanisms. Result(s) The average rates of chromosomally unbalanced spermatozoa were 55.22%, 14.09%, and 18.43% for reciprocal translocation, robertsonian translocation, and pericentric inversion carriers, respectively. The rates of DNA fragmentation were significantly higher in the whole group of carriers of a chromosomal structural abnormality and in each specific group than in the control group. No correlations between sperm DNA fragmentation and parameters of spermogram, age, or percentage of unbalanced chromosomal gametes were found. Conclusion(s) The DNA fragmentation rate depends solely on the presence of a chromosomal structural abnormality, and, therefore, a chromosomal structural abnormality predicts DNA fragmentation. Both meiotic segregation and DNA fragmentation studies should be integrated in the genetic exploration of male carriers of a chromosomal structural abnormality. |
| doi_str_mv | 10.1016/j.fertnstert.2008.06.052 |
| format | Article |
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Design Case series. Setting University hospital. Patient(s) Thirty-seven male carriers of a chromosomal structural abnormality (21 with a balanced reciprocal translocation, 7 with a robertsonian translocation, 9 with a pericentric inversion). Intervention(s) Meiotic segregation was analyzed by the human sperm–hamster oocyte fusion technique or by fluorescent in situ hybridization, and DNA fragmentation was detected by terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling assay. Main Outcome Measure(s) Relationships between abnormal sperm parameters, DNA fragmentation, and meiotic mechanisms. Result(s) The average rates of chromosomally unbalanced spermatozoa were 55.22%, 14.09%, and 18.43% for reciprocal translocation, robertsonian translocation, and pericentric inversion carriers, respectively. The rates of DNA fragmentation were significantly higher in the whole group of carriers of a chromosomal structural abnormality and in each specific group than in the control group. No correlations between sperm DNA fragmentation and parameters of spermogram, age, or percentage of unbalanced chromosomal gametes were found. Conclusion(s) The DNA fragmentation rate depends solely on the presence of a chromosomal structural abnormality, and, therefore, a chromosomal structural abnormality predicts DNA fragmentation. Both meiotic segregation and DNA fragmentation studies should be integrated in the genetic exploration of male carriers of a chromosomal structural abnormality.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2008.06.052</identifier><identifier>PMID: 18706548</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Apoptosis - genetics ; Biological and medical sciences ; Birth control ; Cells, Cultured ; Chromosome Aberrations ; DNA Fragmentation ; FISH ; Gynecology. Andrology. Obstetrics ; Heterozygote ; Humans ; Infertility, Male - genetics ; Infertility, Male - pathology ; Internal Medicine ; Male ; male infertility ; Medical sciences ; Meiosis - genetics ; meiotic segregation ; Obstetrics and Gynecology ; Spermatogenesis - genetics ; Spermatozoa ; Sterility. Assisted procreation ; TUNEL</subject><ispartof>Fertility and sterility, 2009-08, Vol.92 (2), p.583-589</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2009 American Society for Reproductive Medicine</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-d4b528ad9312578e7b81b233b78363d4cb1124b3d7ad238047244bafa36068f13</citedby><cites>FETCH-LOGICAL-c507t-d4b528ad9312578e7b81b233b78363d4cb1124b3d7ad238047244bafa36068f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2008.06.052$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21824515$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18706548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perrin, Aurore, M.Sc</creatorcontrib><creatorcontrib>Caer, Emilie, M.D</creatorcontrib><creatorcontrib>Oliver-Bonet, Maria, M.D</creatorcontrib><creatorcontrib>Navarro, Joaquima, M.D</creatorcontrib><creatorcontrib>Benet, Jordi, M.D</creatorcontrib><creatorcontrib>Amice, Véronique, M.D</creatorcontrib><creatorcontrib>De Braekeleer, Marc, M.D., Ph.D</creatorcontrib><creatorcontrib>Morel, Frédéric, Ph.D</creatorcontrib><title>DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To determine the meiotic segregation and DNA fragmentation in spermatozoa of carriers of a chromosomal structural abnormality. Design Case series. Setting University hospital. Patient(s) Thirty-seven male carriers of a chromosomal structural abnormality (21 with a balanced reciprocal translocation, 7 with a robertsonian translocation, 9 with a pericentric inversion). Intervention(s) Meiotic segregation was analyzed by the human sperm–hamster oocyte fusion technique or by fluorescent in situ hybridization, and DNA fragmentation was detected by terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling assay. Main Outcome Measure(s) Relationships between abnormal sperm parameters, DNA fragmentation, and meiotic mechanisms. Result(s) The average rates of chromosomally unbalanced spermatozoa were 55.22%, 14.09%, and 18.43% for reciprocal translocation, robertsonian translocation, and pericentric inversion carriers, respectively. The rates of DNA fragmentation were significantly higher in the whole group of carriers of a chromosomal structural abnormality and in each specific group than in the control group. No correlations between sperm DNA fragmentation and parameters of spermogram, age, or percentage of unbalanced chromosomal gametes were found. Conclusion(s) The DNA fragmentation rate depends solely on the presence of a chromosomal structural abnormality, and, therefore, a chromosomal structural abnormality predicts DNA fragmentation. Both meiotic segregation and DNA fragmentation studies should be integrated in the genetic exploration of male carriers of a chromosomal structural abnormality.</description><subject>Apoptosis - genetics</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cells, Cultured</subject><subject>Chromosome Aberrations</subject><subject>DNA Fragmentation</subject><subject>FISH</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Heterozygote</subject><subject>Humans</subject><subject>Infertility, Male - genetics</subject><subject>Infertility, Male - pathology</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>male infertility</subject><subject>Medical sciences</subject><subject>Meiosis - genetics</subject><subject>meiotic segregation</subject><subject>Obstetrics and Gynecology</subject><subject>Spermatogenesis - genetics</subject><subject>Spermatozoa</subject><subject>Sterility. Assisted procreation</subject><subject>TUNEL</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQQIMo7rj6FyQXvXVbSTrpeBHWVXeFRQ_qOaTT1WPG7mRM0gvz780wgwuevCRF8eqDRxFCGbQMmHqzaydMJeRS35YD6BZUC5I_IhsmpWqkkuIx2QAw2QDX_II8y3kHAIr1_Cm5YLoHJTu9IdOHL1d0Sna7YCi2-BioDSNd0MfiHc24Tbg95X2geY9poXGizqbkMeVjbKn7meISc1zsTHNJqytrqqEdQkw158vhOXky2Tnji_N_SX58-vj9-ra5-3rz-frqrnES-tKM3SC5tuNbwbjsNfaDZgMXYui1UGLs3MAY7wYx9nbkQkPX864b7GSFAqUnJi7J61PffYq_V8zFLD47nGcbMK7ZqF52kjFVQX0CXYo5J5zMPvnFpoNhYI6Ozc48ODZHxwaUqY5r6cvzjHVYcHwoPEutwKszYLOzc7UbnM9_Oc40r0vIyr0_cViN3FedJjuPweHoE7pixuj_Z5t3_zRxsw--zv2FB8y7uKZQjRtmMjdgvh1v4ngSoIF1rBPiD_HdtYU</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Perrin, Aurore, M.Sc</creator><creator>Caer, Emilie, M.D</creator><creator>Oliver-Bonet, Maria, M.D</creator><creator>Navarro, Joaquima, M.D</creator><creator>Benet, Jordi, M.D</creator><creator>Amice, Véronique, M.D</creator><creator>De Braekeleer, Marc, M.D., Ph.D</creator><creator>Morel, Frédéric, Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality</title><author>Perrin, Aurore, M.Sc ; Caer, Emilie, M.D ; Oliver-Bonet, Maria, M.D ; Navarro, Joaquima, M.D ; Benet, Jordi, M.D ; Amice, Véronique, M.D ; De Braekeleer, Marc, M.D., Ph.D ; Morel, Frédéric, Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-d4b528ad9312578e7b81b233b78363d4cb1124b3d7ad238047244bafa36068f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Apoptosis - genetics</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cells, Cultured</topic><topic>Chromosome Aberrations</topic><topic>DNA Fragmentation</topic><topic>FISH</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Heterozygote</topic><topic>Humans</topic><topic>Infertility, Male - genetics</topic><topic>Infertility, Male - pathology</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>male infertility</topic><topic>Medical sciences</topic><topic>Meiosis - genetics</topic><topic>meiotic segregation</topic><topic>Obstetrics and Gynecology</topic><topic>Spermatogenesis - genetics</topic><topic>Spermatozoa</topic><topic>Sterility. Assisted procreation</topic><topic>TUNEL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perrin, Aurore, M.Sc</creatorcontrib><creatorcontrib>Caer, Emilie, M.D</creatorcontrib><creatorcontrib>Oliver-Bonet, Maria, M.D</creatorcontrib><creatorcontrib>Navarro, Joaquima, M.D</creatorcontrib><creatorcontrib>Benet, Jordi, M.D</creatorcontrib><creatorcontrib>Amice, Véronique, M.D</creatorcontrib><creatorcontrib>De Braekeleer, Marc, M.D., Ph.D</creatorcontrib><creatorcontrib>Morel, Frédéric, Ph.D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perrin, Aurore, M.Sc</au><au>Caer, Emilie, M.D</au><au>Oliver-Bonet, Maria, M.D</au><au>Navarro, Joaquima, M.D</au><au>Benet, Jordi, M.D</au><au>Amice, Véronique, M.D</au><au>De Braekeleer, Marc, M.D., Ph.D</au><au>Morel, Frédéric, Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>92</volume><issue>2</issue><spage>583</spage><epage>589</epage><pages>583-589</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective To determine the meiotic segregation and DNA fragmentation in spermatozoa of carriers of a chromosomal structural abnormality. Design Case series. Setting University hospital. Patient(s) Thirty-seven male carriers of a chromosomal structural abnormality (21 with a balanced reciprocal translocation, 7 with a robertsonian translocation, 9 with a pericentric inversion). Intervention(s) Meiotic segregation was analyzed by the human sperm–hamster oocyte fusion technique or by fluorescent in situ hybridization, and DNA fragmentation was detected by terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling assay. Main Outcome Measure(s) Relationships between abnormal sperm parameters, DNA fragmentation, and meiotic mechanisms. Result(s) The average rates of chromosomally unbalanced spermatozoa were 55.22%, 14.09%, and 18.43% for reciprocal translocation, robertsonian translocation, and pericentric inversion carriers, respectively. The rates of DNA fragmentation were significantly higher in the whole group of carriers of a chromosomal structural abnormality and in each specific group than in the control group. No correlations between sperm DNA fragmentation and parameters of spermogram, age, or percentage of unbalanced chromosomal gametes were found. Conclusion(s) The DNA fragmentation rate depends solely on the presence of a chromosomal structural abnormality, and, therefore, a chromosomal structural abnormality predicts DNA fragmentation. Both meiotic segregation and DNA fragmentation studies should be integrated in the genetic exploration of male carriers of a chromosomal structural abnormality.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18706548</pmid><doi>10.1016/j.fertnstert.2008.06.052</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis - genetics Biological and medical sciences Birth control Cells, Cultured Chromosome Aberrations DNA Fragmentation FISH Gynecology. Andrology. Obstetrics Heterozygote Humans Infertility, Male - genetics Infertility, Male - pathology Internal Medicine Male male infertility Medical sciences Meiosis - genetics meiotic segregation Obstetrics and Gynecology Spermatogenesis - genetics Spermatozoa Sterility. Assisted procreation TUNEL |
| title | DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality |
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