Real-time microbe detection based on director distortions around growing immune complexes in lyotropic chromonic liquid crystals
We describe director distortions in the nematic liquid crystal (LC) caused by a spherical particle with tangential surface orientation of the director and show that light transmittance through the distorted region is a steep function of the particle's size. The effect allows us to propose a rea...
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| Veröffentlicht in: | Physical review. E, Statistical, nonlinear, and soft matter physics Statistical, nonlinear, and soft matter physics, 2005-02, Vol.71 (2 Pt 1), p.020702-020702, Article 020702 |
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| container_end_page | 020702 |
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| container_issue | 2 Pt 1 |
| container_start_page | 020702 |
| container_title | Physical review. E, Statistical, nonlinear, and soft matter physics |
| container_volume | 71 |
| creator | Shiyanovskii, S V Schneider, T Smalyukh, I I Ishikawa, T Niehaus, G D Doane, K J Woolverton, C J Lavrentovich, O D |
| description | We describe director distortions in the nematic liquid crystal (LC) caused by a spherical particle with tangential surface orientation of the director and show that light transmittance through the distorted region is a steep function of the particle's size. The effect allows us to propose a real-time microbial sensor based on a nontoxic lyotropic chromonic LC (LCLC) that detects and amplifies the presence of immune complexes. A cassette is filled with LCLC, antibody, and antigen-bearing particles. Small and isolated particles cause no macroscopic distortions of the LCLC. Upon antibody-antigen binding, the growing immune complexes distort the director and cause detectable optical transmittance between crossed polarizers. |
| doi_str_mv | 10.1103/physreve.71.020702 |
| format | Article |
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E, Statistical, nonlinear, and soft matter physics</jtitle><addtitle>Phys Rev E Stat Nonlin Soft Matter Phys</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>71</volume><issue>2 Pt 1</issue><spage>020702</spage><epage>020702</epage><pages>020702-020702</pages><artnum>020702</artnum><issn>1539-3755</issn><eissn>1550-2376</eissn><abstract>We describe director distortions in the nematic liquid crystal (LC) caused by a spherical particle with tangential surface orientation of the director and show that light transmittance through the distorted region is a steep function of the particle's size. The effect allows us to propose a real-time microbial sensor based on a nontoxic lyotropic chromonic LC (LCLC) that detects and amplifies the presence of immune complexes. A cassette is filled with LCLC, antibody, and antigen-bearing particles. Small and isolated particles cause no macroscopic distortions of the LCLC. 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| ispartof | Physical review. E, Statistical, nonlinear, and soft matter physics, 2005-02, Vol.71 (2 Pt 1), p.020702-020702, Article 020702 |
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| source | MEDLINE; American Physical Society Journals |
| subjects | Antigen-Antibody Complex - analysis Bacteria - cytology Bacteria - immunology Bacteria - isolation & purification Biosensing Techniques - instrumentation Biosensing Techniques - methods Colony Count, Microbial - instrumentation Colony Count, Microbial - methods Computer Systems Immunoassay - methods Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microscopy, Polarization - instrumentation Microscopy, Polarization - methods Reproducibility of Results Sensitivity and Specificity |
| title | Real-time microbe detection based on director distortions around growing immune complexes in lyotropic chromonic liquid crystals |
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