Quadruplex Formation by a Guanine-Rich PNA Oligomer
A guanine-rich PNA dodecamer having the sequence H-G4T4G4-Lys-NH2 (G 4 -PNA) hybridizes with a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt, C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111−4118). This report describes quadruplex formation by the P...
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| Veröffentlicht in: | Journal of the American Chemical Society 2005-03, Vol.127 (12), p.4199-4207 |
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| container_end_page | 4207 |
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| container_issue | 12 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 127 |
| creator | Datta, Bhaskar Bier, Mark E Roy, Subhadeep Armitage, Bruce A |
| description | A guanine-rich PNA dodecamer having the sequence H-G4T4G4-Lys-NH2 (G 4 -PNA) hybridizes with a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt, C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111−4118). This report describes quadruplex formation by the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments reveal formation of a multistranded structure stabilized by guanine tetrads. The ion dependency of these structures is analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicates that both dimeric and tetrameric quadruplexes are formed by G 4 -PNA, with the dimeric form being preferred. These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targets in chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipid bilayer environments. |
| doi_str_mv | 10.1021/ja0446202 |
| format | Article |
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A. J. Am. Chem. Soc. 2003, 125, 4111−4118). This report describes quadruplex formation by the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments reveal formation of a multistranded structure stabilized by guanine tetrads. The ion dependency of these structures is analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicates that both dimeric and tetrameric quadruplexes are formed by G 4 -PNA, with the dimeric form being preferred. These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targets in chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipid bilayer environments.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja0446202</identifier><identifier>PMID: 15783201</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA - chemistry ; Fundamental and applied biological sciences. Psychology ; G-Quadruplexes ; Guanine - chemistry ; Molecular biophysics ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Peptide Nucleic Acids - chemistry ; Spectrometry, Fluorescence ; Spectrometry, Mass, Electrospray Ionization ; Spectrophotometry, Ultraviolet ; Structure in molecular biology ; Supramolecular structure</subject><ispartof>Journal of the American Chemical Society, 2005-03, Vol.127 (12), p.4199-4207</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a412t-3c24518cbf793fa7f0ae6de97afb31dc081b0d8129d30bdaa40b1217664d104c3</citedby><cites>FETCH-LOGICAL-a412t-3c24518cbf793fa7f0ae6de97afb31dc081b0d8129d30bdaa40b1217664d104c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja0446202$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja0446202$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16634607$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15783201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Datta, Bhaskar</creatorcontrib><creatorcontrib>Bier, Mark E</creatorcontrib><creatorcontrib>Roy, Subhadeep</creatorcontrib><creatorcontrib>Armitage, Bruce A</creatorcontrib><title>Quadruplex Formation by a Guanine-Rich PNA Oligomer</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>A guanine-rich PNA dodecamer having the sequence H-G4T4G4-Lys-NH2 (G 4 -PNA) hybridizes with a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt, C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111−4118). This report describes quadruplex formation by the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments reveal formation of a multistranded structure stabilized by guanine tetrads. The ion dependency of these structures is analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicates that both dimeric and tetrameric quadruplexes are formed by G 4 -PNA, with the dimeric form being preferred. These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targets in chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipid bilayer environments.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G-Quadruplexes</subject><subject>Guanine - chemistry</subject><subject>Molecular biophysics</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Hybridization</subject><subject>Peptide Nucleic Acids - chemistry</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Structure in molecular biology</subject><subject>Supramolecular structure</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtLxDAQB_Agiq6Pg19AelHwUJ1J0qR7lMVVwff7FqZpql37WJMt6Le3sot78TQM8-PP8GdsF-EIgePxhEBKxYGvsAEmHOIEuVplAwDgsU6V2GCbIUz6VfIU19kGJjoVHHDAxF1Hue-mlfuKxq2vaVa2TZR9RxSdddSUjYvvS_se3V6fRDdV-dbWzm-ztYKq4HYWc4s9jU8fR-fx5c3ZxejkMiaJfBYLy2WCqc0KPRQF6QLIqdwNNRWZwNxCihnkKfJhLiDLiSRkyFErJXMEacUWO5jnTn372bkwM3UZrKsqalzbBaN0IkGItIeHc2h9G4J3hZn6sib_bRDMb0Pmr6He7i1Cu6x2-VIuKunB_gJQsFQVnhpbhqVTSkgFunfx3JVh5r7-7uQ_-seETszj7YPBlytxNX59NtfLXLLBTNrON313_zz4AxsBhiA</recordid><startdate>20050330</startdate><enddate>20050330</enddate><creator>Datta, Bhaskar</creator><creator>Bier, Mark E</creator><creator>Roy, Subhadeep</creator><creator>Armitage, Bruce A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050330</creationdate><title>Quadruplex Formation by a Guanine-Rich PNA Oligomer</title><author>Datta, Bhaskar ; Bier, Mark E ; Roy, Subhadeep ; Armitage, Bruce A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a412t-3c24518cbf793fa7f0ae6de97afb31dc081b0d8129d30bdaa40b1217664d104c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-Quadruplexes</topic><topic>Guanine - chemistry</topic><topic>Molecular biophysics</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Hybridization</topic><topic>Peptide Nucleic Acids - chemistry</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Structure in molecular biology</topic><topic>Supramolecular structure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Datta, Bhaskar</creatorcontrib><creatorcontrib>Bier, Mark E</creatorcontrib><creatorcontrib>Roy, Subhadeep</creatorcontrib><creatorcontrib>Armitage, Bruce A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Datta, Bhaskar</au><au>Bier, Mark E</au><au>Roy, Subhadeep</au><au>Armitage, Bruce A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quadruplex Formation by a Guanine-Rich PNA Oligomer</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2005-03-30</date><risdate>2005</risdate><volume>127</volume><issue>12</issue><spage>4199</spage><epage>4207</epage><pages>4199-4207</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>A guanine-rich PNA dodecamer having the sequence H-G4T4G4-Lys-NH2 (G 4 -PNA) hybridizes with a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt, C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111−4118). This report describes quadruplex formation by the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments reveal formation of a multistranded structure stabilized by guanine tetrads. The ion dependency of these structures is analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicates that both dimeric and tetrameric quadruplexes are formed by G 4 -PNA, with the dimeric form being preferred. These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targets in chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipid bilayer environments.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15783201</pmid><doi>10.1021/ja0446202</doi><tpages>9</tpages></addata></record> |
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| subjects | Base Sequence Biological and medical sciences DNA - chemistry Fundamental and applied biological sciences. Psychology G-Quadruplexes Guanine - chemistry Molecular biophysics Nucleic Acid Conformation Nucleic Acid Hybridization Peptide Nucleic Acids - chemistry Spectrometry, Fluorescence Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Ultraviolet Structure in molecular biology Supramolecular structure |
| title | Quadruplex Formation by a Guanine-Rich PNA Oligomer |
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