Extracellular ascorbate modulates cortically evoked glutamate dynamics in rat striatum
To determine if extracellular ascorbate, which may increase by several hundred micromolar in striatum during behavioral activation, directly alters glutamate transmission, we monitored striatal glutamate transients evoked by electrical stimulation of cerebral cortex in anesthetized rats tested with...
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| Veröffentlicht in: | Neuroscience letters 2005-04, Vol.378 (3), p.166-170 |
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| creator | Rebec, George V. Witowski, Steven R. Sandstrom, Michael I. Rostand, Rebecca D. Kennedy, Robert T. |
| description | To determine if extracellular ascorbate, which may increase by several hundred micromolar in striatum during behavioral activation, directly alters glutamate transmission, we monitored striatal glutamate transients evoked by electrical stimulation of cerebral cortex in anesthetized rats tested with varying concentrations of ascorbate (0, 50, 200, and 500
μM) by reverse dialysis. Capillary electrophoresis coupled with laser-induced fluorescence detection was used to analyze dialysates collected at 3-s intervals. Ascorbate elevated striatal glutamate in a concentration-dependent fashion. Addition of 500
μM ascorbate not only more than doubled basal glutamate levels relative to the ascorbate-free condition, but significantly increased both the magnitude of the electrically evoked glutamate response as well as its subsequent return to baseline. In fact, the time required to return to within 10% of the pre-stimulation baseline increased by >100
s. Reverse dialysis of
iso-ascorbate, in contrast, had no effect on stimulation-evoked glutamate release arguing against an antioxidant effect. It appears, therefore, that the level of extracellular ascorbate plays a critical role in regulating corticostriatal glutamate transmission. |
| doi_str_mv | 10.1016/j.neulet.2004.12.027 |
| format | Article |
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μM) by reverse dialysis. Capillary electrophoresis coupled with laser-induced fluorescence detection was used to analyze dialysates collected at 3-s intervals. Ascorbate elevated striatal glutamate in a concentration-dependent fashion. Addition of 500
μM ascorbate not only more than doubled basal glutamate levels relative to the ascorbate-free condition, but significantly increased both the magnitude of the electrically evoked glutamate response as well as its subsequent return to baseline. In fact, the time required to return to within 10% of the pre-stimulation baseline increased by >100
s. Reverse dialysis of
iso-ascorbate, in contrast, had no effect on stimulation-evoked glutamate release arguing against an antioxidant effect. It appears, therefore, that the level of extracellular ascorbate plays a critical role in regulating corticostriatal glutamate transmission.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2004.12.027</identifier><identifier>PMID: 15781152</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Ascorbate ; Ascorbic Acid - metabolism ; Ascorbic Acid - pharmacology ; Biological and medical sciences ; Capillary electrophoresis ; Cerebral cortex ; Cerebral Cortex - drug effects ; Cerebral Cortex - metabolism ; Corpus Striatum - drug effects ; Corpus Striatum - metabolism ; Dose-Response Relationship, Drug ; Electric Stimulation ; Extracellular Fluid - metabolism ; Fundamental and applied biological sciences. Psychology ; Glutamate ; Glutamic Acid - metabolism ; Heteroexchange ; Male ; Microdialysis ; Neural Pathways - drug effects ; Neural Pathways - metabolism ; Neurons - drug effects ; Neurons - metabolism ; Rats ; Rats, Sprague-Dawley ; Striatum ; Synaptic Transmission - drug effects ; Synaptic Transmission - physiology ; Up-Regulation - drug effects ; Up-Regulation - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2005-04, Vol.378 (3), p.166-170</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-bcc30ea642567678ffcb0bcb3979a7cc35296cfc1b25b4af786caf05ae2a61203</citedby><cites>FETCH-LOGICAL-c390t-bcc30ea642567678ffcb0bcb3979a7cc35296cfc1b25b4af786caf05ae2a61203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304394004015514$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16626965$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15781152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rebec, George V.</creatorcontrib><creatorcontrib>Witowski, Steven R.</creatorcontrib><creatorcontrib>Sandstrom, Michael I.</creatorcontrib><creatorcontrib>Rostand, Rebecca D.</creatorcontrib><creatorcontrib>Kennedy, Robert T.</creatorcontrib><title>Extracellular ascorbate modulates cortically evoked glutamate dynamics in rat striatum</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>To determine if extracellular ascorbate, which may increase by several hundred micromolar in striatum during behavioral activation, directly alters glutamate transmission, we monitored striatal glutamate transients evoked by electrical stimulation of cerebral cortex in anesthetized rats tested with varying concentrations of ascorbate (0, 50, 200, and 500
μM) by reverse dialysis. Capillary electrophoresis coupled with laser-induced fluorescence detection was used to analyze dialysates collected at 3-s intervals. Ascorbate elevated striatal glutamate in a concentration-dependent fashion. Addition of 500
μM ascorbate not only more than doubled basal glutamate levels relative to the ascorbate-free condition, but significantly increased both the magnitude of the electrically evoked glutamate response as well as its subsequent return to baseline. In fact, the time required to return to within 10% of the pre-stimulation baseline increased by >100
s. Reverse dialysis of
iso-ascorbate, in contrast, had no effect on stimulation-evoked glutamate release arguing against an antioxidant effect. It appears, therefore, that the level of extracellular ascorbate plays a critical role in regulating corticostriatal glutamate transmission.</description><subject>Animals</subject><subject>Ascorbate</subject><subject>Ascorbic Acid - metabolism</subject><subject>Ascorbic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Capillary electrophoresis</subject><subject>Cerebral cortex</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electric Stimulation</subject><subject>Extracellular Fluid - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamate</subject><subject>Glutamic Acid - metabolism</subject><subject>Heteroexchange</subject><subject>Male</subject><subject>Microdialysis</subject><subject>Neural Pathways - drug effects</subject><subject>Neural Pathways - metabolism</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Striatum</subject><subject>Synaptic Transmission - drug effects</subject><subject>Synaptic Transmission - physiology</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2P1DAMhiMEYoeFf4BQL3BrcdImmV6Q0Gr5kFbiAlwj13VRhrRdknTF_HsympH2xsmS_djy-wjxWkIjQZr3h2bhLXBuFEDXSNWAsk_ETu6tqm1v1VOxgxa6uu07uBIvUjoAgJa6ey6upLZ7KbXaiZ-3f3NE4hC2gLHCRGscMHM1r2PpZE5V6WRPGMKx4of1N4_Vr7BlnE_UeFxw9pQqv1QRc5Vy9Ji3-aV4NmFI_OpSr8WPT7ffb77Ud98-f735eFdT20OuB6IWGE2ntLHG7qeJBhhoaHvboy1DrXpDE8lB6aHDye4N4QQaWaGRCtpr8e589z6ufzZO2c0-ndLgwuuWnLG67UrUAnZnkOKaUuTJ3Uc_Yzw6Ce7k0x3c2ac7-XRSueKzrL253N-GmcfHpYvAAry9AEUdhiniQj49csYo0xtduA9njouNB8_RJfK8EI8-MmU3rv7_n_wDAS-XxQ</recordid><startdate>20050422</startdate><enddate>20050422</enddate><creator>Rebec, George V.</creator><creator>Witowski, Steven R.</creator><creator>Sandstrom, Michael I.</creator><creator>Rostand, Rebecca D.</creator><creator>Kennedy, Robert T.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050422</creationdate><title>Extracellular ascorbate modulates cortically evoked glutamate dynamics in rat striatum</title><author>Rebec, George V. ; Witowski, Steven R. ; Sandstrom, Michael I. ; Rostand, Rebecca D. ; Kennedy, Robert T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-bcc30ea642567678ffcb0bcb3979a7cc35296cfc1b25b4af786caf05ae2a61203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Ascorbate</topic><topic>Ascorbic Acid - metabolism</topic><topic>Ascorbic Acid - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Capillary electrophoresis</topic><topic>Cerebral cortex</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electric Stimulation</topic><topic>Extracellular Fluid - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamate</topic><topic>Glutamic Acid - metabolism</topic><topic>Heteroexchange</topic><topic>Male</topic><topic>Microdialysis</topic><topic>Neural Pathways - drug effects</topic><topic>Neural Pathways - metabolism</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Striatum</topic><topic>Synaptic Transmission - drug effects</topic><topic>Synaptic Transmission - physiology</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rebec, George V.</creatorcontrib><creatorcontrib>Witowski, Steven R.</creatorcontrib><creatorcontrib>Sandstrom, Michael I.</creatorcontrib><creatorcontrib>Rostand, Rebecca D.</creatorcontrib><creatorcontrib>Kennedy, Robert T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rebec, George V.</au><au>Witowski, Steven R.</au><au>Sandstrom, Michael I.</au><au>Rostand, Rebecca D.</au><au>Kennedy, Robert T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular ascorbate modulates cortically evoked glutamate dynamics in rat striatum</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2005-04-22</date><risdate>2005</risdate><volume>378</volume><issue>3</issue><spage>166</spage><epage>170</epage><pages>166-170</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>To determine if extracellular ascorbate, which may increase by several hundred micromolar in striatum during behavioral activation, directly alters glutamate transmission, we monitored striatal glutamate transients evoked by electrical stimulation of cerebral cortex in anesthetized rats tested with varying concentrations of ascorbate (0, 50, 200, and 500
μM) by reverse dialysis. Capillary electrophoresis coupled with laser-induced fluorescence detection was used to analyze dialysates collected at 3-s intervals. Ascorbate elevated striatal glutamate in a concentration-dependent fashion. Addition of 500
μM ascorbate not only more than doubled basal glutamate levels relative to the ascorbate-free condition, but significantly increased both the magnitude of the electrically evoked glutamate response as well as its subsequent return to baseline. In fact, the time required to return to within 10% of the pre-stimulation baseline increased by >100
s. Reverse dialysis of
iso-ascorbate, in contrast, had no effect on stimulation-evoked glutamate release arguing against an antioxidant effect. It appears, therefore, that the level of extracellular ascorbate plays a critical role in regulating corticostriatal glutamate transmission.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15781152</pmid><doi>10.1016/j.neulet.2004.12.027</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Ascorbate Ascorbic Acid - metabolism Ascorbic Acid - pharmacology Biological and medical sciences Capillary electrophoresis Cerebral cortex Cerebral Cortex - drug effects Cerebral Cortex - metabolism Corpus Striatum - drug effects Corpus Striatum - metabolism Dose-Response Relationship, Drug Electric Stimulation Extracellular Fluid - metabolism Fundamental and applied biological sciences. Psychology Glutamate Glutamic Acid - metabolism Heteroexchange Male Microdialysis Neural Pathways - drug effects Neural Pathways - metabolism Neurons - drug effects Neurons - metabolism Rats Rats, Sprague-Dawley Striatum Synaptic Transmission - drug effects Synaptic Transmission - physiology Up-Regulation - drug effects Up-Regulation - physiology Vertebrates: nervous system and sense organs |
| title | Extracellular ascorbate modulates cortically evoked glutamate dynamics in rat striatum |
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