Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model
Background/Aim: Cirrhosis is a long‐term consequence of chronic hepatic injury and no effective therapy is currently available for this disease. Recent reports have shown that the mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, and umbilical cord blood is a rich so...
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| Veröffentlicht in: | Liver international 2009-07, Vol.29 (6), p.898-909 |
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| creator | Jung, Kyung Hee Shin, Hyun Phil Lee, Sun Lim, Yun Jeong Hwang, Soo Han Han, Hoon Park, Hwon Kyum Chung, Joo-Ho Yim, Sung-Vin |
| description | Background/Aim: Cirrhosis is a long‐term consequence of chronic hepatic injury and no effective therapy is currently available for this disease. Recent reports have shown that the mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, and umbilical cord blood is a rich source of MSCs. Hence, we investigated the effect of infusing of human umbilical cord blood‐derived MSCs (HMSCs) in carbon tetrachloride (CCl4)‐induced cirrhosis in a rat model.
Methods: The effect of HMSCs on cirrhosis was evaluated using haematoxylin and eosin and Masson's trichrome staining. To evaluate cirrhosis‐related factors, we measured protein and mRNA expression of transforming growth factor β1 (TGF‐β1), collagen type I and α‐smooth muscle actin (α‐SMA).
Results: Histological findings showed that liver fibrosis in rats was alleviated by HMSCs infusion. Interestingly, CM‐DiI‐labelled HMSCs expressed the hepatocyte‐specific markers, human albumin and α‐fetoprotein. Infusion of HMSCs significantly inhibited TGF‐β1, collagen type I and α‐SMA expressions in CCl4‐induced cirrhotic rats.
Conclusion: Our results showed that HMSCs infusion could improve liver fibrosis in rats with CCl4‐induced cirrhosis, raising the possibility for clinical use of HMSCs in the treatment of cirrhosis. |
| doi_str_mv | 10.1111/j.1478-3231.2009.02031.x |
| format | Article |
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Methods: The effect of HMSCs on cirrhosis was evaluated using haematoxylin and eosin and Masson's trichrome staining. To evaluate cirrhosis‐related factors, we measured protein and mRNA expression of transforming growth factor β1 (TGF‐β1), collagen type I and α‐smooth muscle actin (α‐SMA).
Results: Histological findings showed that liver fibrosis in rats was alleviated by HMSCs infusion. Interestingly, CM‐DiI‐labelled HMSCs expressed the hepatocyte‐specific markers, human albumin and α‐fetoprotein. Infusion of HMSCs significantly inhibited TGF‐β1, collagen type I and α‐SMA expressions in CCl4‐induced cirrhotic rats.
Conclusion: Our results showed that HMSCs infusion could improve liver fibrosis in rats with CCl4‐induced cirrhosis, raising the possibility for clinical use of HMSCs in the treatment of cirrhosis.</description><identifier>ISSN: 1478-3223</identifier><identifier>EISSN: 1478-3231</identifier><identifier>DOI: 10.1111/j.1478-3231.2009.02031.x</identifier><identifier>PMID: 19422480</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actins - metabolism ; Animals ; Blotting, Western ; Carbon Tetrachloride ; Cell- and Tissue-Based Therapy - methods ; cirrhosis ; collagen type I ; Fetal Blood - cytology ; human umbilical cord blood (HUCB) ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Liver - pathology ; Liver Cirrhosis, Experimental - therapy ; Mesenchymal Stem Cell Transplantation ; mesenchymal stem cells (MSCs) ; Mesenchymal Stromal Cells - physiology ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; TGF-β1 ; Transforming Growth Factor beta1 - metabolism ; α-SMA</subject><ispartof>Liver international, 2009-07, Vol.29 (6), p.898-909</ispartof><rights>2009 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5021-f32265bf0dbefb24605a2127961a7b31ea31525960bb7ad0803aa56ac1d1cfd73</citedby><cites>FETCH-LOGICAL-c5021-f32265bf0dbefb24605a2127961a7b31ea31525960bb7ad0803aa56ac1d1cfd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1478-3231.2009.02031.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1478-3231.2009.02031.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19422480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Kyung Hee</creatorcontrib><creatorcontrib>Shin, Hyun Phil</creatorcontrib><creatorcontrib>Lee, Sun</creatorcontrib><creatorcontrib>Lim, Yun Jeong</creatorcontrib><creatorcontrib>Hwang, Soo Han</creatorcontrib><creatorcontrib>Han, Hoon</creatorcontrib><creatorcontrib>Park, Hwon Kyum</creatorcontrib><creatorcontrib>Chung, Joo-Ho</creatorcontrib><creatorcontrib>Yim, Sung-Vin</creatorcontrib><title>Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model</title><title>Liver international</title><addtitle>Liver Int</addtitle><description>Background/Aim: Cirrhosis is a long‐term consequence of chronic hepatic injury and no effective therapy is currently available for this disease. Recent reports have shown that the mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, and umbilical cord blood is a rich source of MSCs. Hence, we investigated the effect of infusing of human umbilical cord blood‐derived MSCs (HMSCs) in carbon tetrachloride (CCl4)‐induced cirrhosis in a rat model.
Methods: The effect of HMSCs on cirrhosis was evaluated using haematoxylin and eosin and Masson's trichrome staining. To evaluate cirrhosis‐related factors, we measured protein and mRNA expression of transforming growth factor β1 (TGF‐β1), collagen type I and α‐smooth muscle actin (α‐SMA).
Results: Histological findings showed that liver fibrosis in rats was alleviated by HMSCs infusion. Interestingly, CM‐DiI‐labelled HMSCs expressed the hepatocyte‐specific markers, human albumin and α‐fetoprotein. Infusion of HMSCs significantly inhibited TGF‐β1, collagen type I and α‐SMA expressions in CCl4‐induced cirrhotic rats.
Conclusion: Our results showed that HMSCs infusion could improve liver fibrosis in rats with CCl4‐induced cirrhosis, raising the possibility for clinical use of HMSCs in the treatment of cirrhosis.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Carbon Tetrachloride</subject><subject>Cell- and Tissue-Based Therapy - methods</subject><subject>cirrhosis</subject><subject>collagen type I</subject><subject>Fetal Blood - cytology</subject><subject>human umbilical cord blood (HUCB)</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Liver - pathology</subject><subject>Liver Cirrhosis, Experimental - therapy</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>mesenchymal stem cells (MSCs)</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>TGF-β1</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>α-SMA</subject><issn>1478-3223</issn><issn>1478-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTtv2zAUhYkiQeMk_QsBp25S-BAla-hQGI5r1GiXPJYCBJ8wHdFMSCmx_32p2nDHlgsvcM93eXkOABCjEudzuylx1UwLSiguCUJtiQjK5e4DmJwaZ6ea0AtwmdIGIdy2DH8EF7itCKmmaAJ-za01qofBwvXgxRYOXrrOKdFBFaKGsgtBF9pE92Y09CaZrVrvfW6n3nioTNcl6LZQQOViXIfeKRhFD33QprsG51Z0yXw63lfg4W5-P_tWrH4ulrOvq0IxRHBh84o1kxZpaawkVY2YIJg0bY1FIyk2gmJGWFsjKRuh0RRRIVgtFNZYWd3QK_D5MPclhtfBpJ57l8bVxNaEIfG6YbRiDP1TmL2sWbYoC6cHoYohpWgsf4nOi7jnGPExAr7ho7t8dHrEWv4nAr7L6M3xjUF6o_-CR8-z4MtB8O46s__vwXy1fByrzBcH3uUIdidexOf8Udow_vRjwReMPZHZ43dO6W8t3aNw</recordid><startdate>200907</startdate><enddate>200907</enddate><creator>Jung, Kyung Hee</creator><creator>Shin, Hyun Phil</creator><creator>Lee, Sun</creator><creator>Lim, Yun Jeong</creator><creator>Hwang, Soo Han</creator><creator>Han, Hoon</creator><creator>Park, Hwon Kyum</creator><creator>Chung, Joo-Ho</creator><creator>Yim, Sung-Vin</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200907</creationdate><title>Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model</title><author>Jung, Kyung Hee ; Shin, Hyun Phil ; Lee, Sun ; Lim, Yun Jeong ; Hwang, Soo Han ; Han, Hoon ; Park, Hwon Kyum ; Chung, Joo-Ho ; Yim, Sung-Vin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5021-f32265bf0dbefb24605a2127961a7b31ea31525960bb7ad0803aa56ac1d1cfd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Carbon Tetrachloride</topic><topic>Cell- and Tissue-Based Therapy - methods</topic><topic>cirrhosis</topic><topic>collagen type I</topic><topic>Fetal Blood - cytology</topic><topic>human umbilical cord blood (HUCB)</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Liver - pathology</topic><topic>Liver Cirrhosis, Experimental - therapy</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>mesenchymal stem cells (MSCs)</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>TGF-β1</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>α-SMA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Kyung Hee</creatorcontrib><creatorcontrib>Shin, Hyun Phil</creatorcontrib><creatorcontrib>Lee, Sun</creatorcontrib><creatorcontrib>Lim, Yun Jeong</creatorcontrib><creatorcontrib>Hwang, Soo Han</creatorcontrib><creatorcontrib>Han, Hoon</creatorcontrib><creatorcontrib>Park, Hwon Kyum</creatorcontrib><creatorcontrib>Chung, Joo-Ho</creatorcontrib><creatorcontrib>Yim, Sung-Vin</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Liver international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Kyung Hee</au><au>Shin, Hyun Phil</au><au>Lee, Sun</au><au>Lim, Yun Jeong</au><au>Hwang, Soo Han</au><au>Han, Hoon</au><au>Park, Hwon Kyum</au><au>Chung, Joo-Ho</au><au>Yim, Sung-Vin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model</atitle><jtitle>Liver international</jtitle><addtitle>Liver Int</addtitle><date>2009-07</date><risdate>2009</risdate><volume>29</volume><issue>6</issue><spage>898</spage><epage>909</epage><pages>898-909</pages><issn>1478-3223</issn><eissn>1478-3231</eissn><abstract>Background/Aim: Cirrhosis is a long‐term consequence of chronic hepatic injury and no effective therapy is currently available for this disease. Recent reports have shown that the mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, and umbilical cord blood is a rich source of MSCs. Hence, we investigated the effect of infusing of human umbilical cord blood‐derived MSCs (HMSCs) in carbon tetrachloride (CCl4)‐induced cirrhosis in a rat model.
Methods: The effect of HMSCs on cirrhosis was evaluated using haematoxylin and eosin and Masson's trichrome staining. To evaluate cirrhosis‐related factors, we measured protein and mRNA expression of transforming growth factor β1 (TGF‐β1), collagen type I and α‐smooth muscle actin (α‐SMA).
Results: Histological findings showed that liver fibrosis in rats was alleviated by HMSCs infusion. Interestingly, CM‐DiI‐labelled HMSCs expressed the hepatocyte‐specific markers, human albumin and α‐fetoprotein. Infusion of HMSCs significantly inhibited TGF‐β1, collagen type I and α‐SMA expressions in CCl4‐induced cirrhotic rats.
Conclusion: Our results showed that HMSCs infusion could improve liver fibrosis in rats with CCl4‐induced cirrhosis, raising the possibility for clinical use of HMSCs in the treatment of cirrhosis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19422480</pmid><doi>10.1111/j.1478-3231.2009.02031.x</doi><tpages>12</tpages></addata></record> |
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| ispartof | Liver international, 2009-07, Vol.29 (6), p.898-909 |
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| language | eng |
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| subjects | Actins - metabolism Animals Blotting, Western Carbon Tetrachloride Cell- and Tissue-Based Therapy - methods cirrhosis collagen type I Fetal Blood - cytology human umbilical cord blood (HUCB) Humans Immunohistochemistry In Situ Hybridization, Fluorescence Liver - pathology Liver Cirrhosis, Experimental - therapy Mesenchymal Stem Cell Transplantation mesenchymal stem cells (MSCs) Mesenchymal Stromal Cells - physiology Rats Reverse Transcriptase Polymerase Chain Reaction TGF-β1 Transforming Growth Factor beta1 - metabolism α-SMA |
| title | Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model |
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