Two-dimensional Blue Native/sodium dodecyl sulfate gel electrophoresis for analysis of multimeric proteins in platelets

Two‐dimensional (2‐D) Blue Native/SDS gel electrophoresis combines a first‐dimensional separation of monomeric and multimeric proteins in their native state with a second denaturing dimension. These high‐resolution 2‐D gels aim at identifying multiprotein complexes with respect to their subunit comp...

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Veröffentlicht in:	Electrophoresis 2005-03, Vol.26 (6), p.1189-1199
Hauptverfasser:	Claeys, Dirk, Geering, Käthi, Meyer, Beat J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blood Platelets - chemistry Blue Native electrophoresis Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Polyacrylamide Gel - methods Humans Membrane proteins Membrane Proteins - blood Membrane Proteins - isolation & purification Microsomes - metabolism Oocytes - metabolism Platelets Protein complexes Protein Structure, Quaternary Proteome - isolation & purification Two-dimensional gel electrophoresis Xenopus
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