Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads
This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enha...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 2009-10, Vol.393 (1), p.56-61 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 61 |
|---|---|
| container_issue | 1 |
| container_start_page | 56 |
| container_title | Analytical biochemistry |
| container_volume | 393 |
| creator | Yang, Xiaoyan Guo, Yingshu Mei, Zhenhua |
| description | This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of TRP were compared with those of p-iodophenol (PIP). TRP produced a strong enhancement of the CL with the effect of prolonging the light emission. The developed system was then applied to the determination of H(2)O(2) with immobilized HRP using magnetic beads as a solid support. The linear range for H(2)O(2) was 2.0x10(-6) to 1.0x10(-3) M. The detection limit for H(2)O(2) was 2.0x10(-6) M. The proposed sensor was applied successfully to the determination of H(2)O(2) in rainwater. |
| doi_str_mv | 10.1016/j.ab.2009.06.008 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67532170</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67532170</sourcerecordid><originalsourceid>FETCH-LOGICAL-c297t-cec9f9c8adf1697ebfcf83ed1fa675a95ccfb6e637634321d5a05aa953d0cc333</originalsourceid><addsrcrecordid>eNpFkUFr3DAQhUVpabZp7z0VnUoLsTOy1vLqWJa2KQRySc5iLI1jLba0lWxo8nP6S6OQpT0NM7z3wZvH2EcBtQChLg819nUDoGtQNcDuFdsI0KoCCfo12wCArBqluzP2LucDgBDbVr1lZ0K3Tae6dsP-7kea_bTOPlC2FBbuaKFUVlx8DDwO_Kq5afiafbjn2-qLuGguttWSPD7GqRLVw_T1OFKIE8fMMXAKIwZLifeYyfGCWEbifp5j7yf_-I86xpQpofN55EdK8Y93xVD0S-Qz3gdavOU9ocvv2ZsBp0wfTvOc3f34fru_qq5vfv7af7uubKO7pbJk9aDtDt0gSmTqBzvsJDkxYEmKurV26BUp2Sm5lY1wLUKL5S4dWCulPGefX7jHFH-vlBcz-_KSacJAcc2mUIqtgyKEF6FNMedEgzkmP2N6MALMcy_mYLA3z70YUKb0UiyfTuy1n8n9N5yKkE8m_YxH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67532170</pqid></control><display><type>article</type><title>Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yang, Xiaoyan ; Guo, Yingshu ; Mei, Zhenhua</creator><creatorcontrib>Yang, Xiaoyan ; Guo, Yingshu ; Mei, Zhenhua</creatorcontrib><description>This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of TRP were compared with those of p-iodophenol (PIP). TRP produced a strong enhancement of the CL with the effect of prolonging the light emission. The developed system was then applied to the determination of H(2)O(2) with immobilized HRP using magnetic beads as a solid support. The linear range for H(2)O(2) was 2.0x10(-6) to 1.0x10(-3) M. The detection limit for H(2)O(2) was 2.0x10(-6) M. The proposed sensor was applied successfully to the determination of H(2)O(2) in rainwater.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2009.06.008</identifier><identifier>PMID: 19527675</identifier><language>eng</language><publisher>United States</publisher><subject>Armoracia - enzymology ; Biosensing Techniques ; Calibration ; Enzymes, Immobilized - metabolism ; Fresh Water - chemistry ; Horseradish Peroxidase - metabolism ; Hydrogen Peroxide - analysis ; Hydrogen Peroxide - chemistry ; Hydrogen Peroxide - metabolism ; Luminescent Measurements - methods ; Magnetics ; Molecular Structure ; Phenols - chemistry ; Triazoles - chemistry</subject><ispartof>Analytical biochemistry, 2009-10, Vol.393 (1), p.56-61</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c297t-cec9f9c8adf1697ebfcf83ed1fa675a95ccfb6e637634321d5a05aa953d0cc333</citedby><cites>FETCH-LOGICAL-c297t-cec9f9c8adf1697ebfcf83ed1fa675a95ccfb6e637634321d5a05aa953d0cc333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19527675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiaoyan</creatorcontrib><creatorcontrib>Guo, Yingshu</creatorcontrib><creatorcontrib>Mei, Zhenhua</creatorcontrib><title>Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of TRP were compared with those of p-iodophenol (PIP). TRP produced a strong enhancement of the CL with the effect of prolonging the light emission. The developed system was then applied to the determination of H(2)O(2) with immobilized HRP using magnetic beads as a solid support. The linear range for H(2)O(2) was 2.0x10(-6) to 1.0x10(-3) M. The detection limit for H(2)O(2) was 2.0x10(-6) M. The proposed sensor was applied successfully to the determination of H(2)O(2) in rainwater.</description><subject>Armoracia - enzymology</subject><subject>Biosensing Techniques</subject><subject>Calibration</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Fresh Water - chemistry</subject><subject>Horseradish Peroxidase - metabolism</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Luminescent Measurements - methods</subject><subject>Magnetics</subject><subject>Molecular Structure</subject><subject>Phenols - chemistry</subject><subject>Triazoles - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUFr3DAQhUVpabZp7z0VnUoLsTOy1vLqWJa2KQRySc5iLI1jLba0lWxo8nP6S6OQpT0NM7z3wZvH2EcBtQChLg819nUDoGtQNcDuFdsI0KoCCfo12wCArBqluzP2LucDgBDbVr1lZ0K3Tae6dsP-7kea_bTOPlC2FBbuaKFUVlx8DDwO_Kq5afiafbjn2-qLuGguttWSPD7GqRLVw_T1OFKIE8fMMXAKIwZLifeYyfGCWEbifp5j7yf_-I86xpQpofN55EdK8Y93xVD0S-Qz3gdavOU9ocvv2ZsBp0wfTvOc3f34fru_qq5vfv7af7uubKO7pbJk9aDtDt0gSmTqBzvsJDkxYEmKurV26BUp2Sm5lY1wLUKL5S4dWCulPGefX7jHFH-vlBcz-_KSacJAcc2mUIqtgyKEF6FNMedEgzkmP2N6MALMcy_mYLA3z70YUKb0UiyfTuy1n8n9N5yKkE8m_YxH</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Yang, Xiaoyan</creator><creator>Guo, Yingshu</creator><creator>Mei, Zhenhua</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads</title><author>Yang, Xiaoyan ; Guo, Yingshu ; Mei, Zhenhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-cec9f9c8adf1697ebfcf83ed1fa675a95ccfb6e637634321d5a05aa953d0cc333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Armoracia - enzymology</topic><topic>Biosensing Techniques</topic><topic>Calibration</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Fresh Water - chemistry</topic><topic>Horseradish Peroxidase - metabolism</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Luminescent Measurements - methods</topic><topic>Magnetics</topic><topic>Molecular Structure</topic><topic>Phenols - chemistry</topic><topic>Triazoles - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiaoyan</creatorcontrib><creatorcontrib>Guo, Yingshu</creatorcontrib><creatorcontrib>Mei, Zhenhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiaoyan</au><au>Guo, Yingshu</au><au>Mei, Zhenhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>393</volume><issue>1</issue><spage>56</spage><epage>61</epage><pages>56-61</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of TRP were compared with those of p-iodophenol (PIP). TRP produced a strong enhancement of the CL with the effect of prolonging the light emission. The developed system was then applied to the determination of H(2)O(2) with immobilized HRP using magnetic beads as a solid support. The linear range for H(2)O(2) was 2.0x10(-6) to 1.0x10(-3) M. The detection limit for H(2)O(2) was 2.0x10(-6) M. The proposed sensor was applied successfully to the determination of H(2)O(2) in rainwater.</abstract><cop>United States</cop><pmid>19527675</pmid><doi>10.1016/j.ab.2009.06.008</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 2009-10, Vol.393 (1), p.56-61 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67532170 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Armoracia - enzymology Biosensing Techniques Calibration Enzymes, Immobilized - metabolism Fresh Water - chemistry Horseradish Peroxidase - metabolism Hydrogen Peroxide - analysis Hydrogen Peroxide - chemistry Hydrogen Peroxide - metabolism Luminescent Measurements - methods Magnetics Molecular Structure Phenols - chemistry Triazoles - chemistry |
| title | Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T20%3A05%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chemiluminescent%20determination%20of%20H2O2%20using%204-(1,2,4-triazol-1-yl)phenol%20as%20an%20enhancer%20based%20on%20the%20immobilization%20of%20horseradish%20peroxidase%20onto%20magnetic%20beads&rft.jtitle=Analytical%20biochemistry&rft.au=Yang,%20Xiaoyan&rft.date=2009-10-01&rft.volume=393&rft.issue=1&rft.spage=56&rft.epage=61&rft.pages=56-61&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/j.ab.2009.06.008&rft_dat=%3Cproquest_cross%3E67532170%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67532170&rft_id=info:pmid/19527675&rfr_iscdi=true |