Transport of live yeast and zebrafish embryo on a droplet digital microfluidic platform
We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transp...
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| Veröffentlicht in: | Lab on a chip 2009-08, Vol.9 (16), p.2398-2401 |
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| container_title | Lab on a chip |
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| creator | Son, Sang Uk Garrell, Robin L |
| description | We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays. |
| doi_str_mv | 10.1039/b906257b |
| format | Article |
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The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. 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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2009-08, Vol.9 (16), p.2398-2401 |
| issn | 1473-0197 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Embryo Culture Techniques Microfluidic Analytical Techniques - methods Motion Preservation, Biological - methods Saccharomyces cerevisiae - cytology Zebrafish - embryology |
| title | Transport of live yeast and zebrafish embryo on a droplet digital microfluidic platform |
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