Decrypting the Biochemical Function of an Essential Gene from Streptococcus pneumoniae Using ThermoFluor® Technology

The protein product of an essential gene of unknown function from Streptococcus pneumoniae was expressed and purified for screening in the ThermoFluor® affinity screening assay. This assay can detect ligand binding to proteins of unknown function. The recombinant protein was found to be in a dimeric...

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Veröffentlicht in:	The Journal of biological chemistry 2005-03, Vol.280 (12), p.11704-11712
Hauptverfasser:	Carver, Theodore E., Bordeau, Brian, Cummings, Maxwell D., Petrella, Eugene C., Pucci, Michael J., Zawadzke, Laura E., Dougherty, Brian A., Tredup, Jeffrey A., Bryson, James W., Yanchunas, Joseph, Doyle, Michael L., Witmer, Mark R., Nelen, Marina I., DesJarlais, Renee L., Jaeger, Edward P., Devine, Heather, Asel, Eric D., Springer, Barry A., Bone, Roger, Salemme, F. Raymond, Todd, Matthew J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - physiology Benzopyrans - metabolism Dimerization Furans - metabolism Genes, Essential - physiology Ligands Molecular Sequence Data Nucleoside Diphosphate Sugars - metabolism Pyridoxal Phosphate - metabolism Pyridoxamine - metabolism Streptococcus pneumoniae - enzymology Streptococcus pneumoniae - genetics Transaminases - physiology
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