Identifying subpopulations of thymic epithelial cells by flow cytometry using a new specific thymic epithelial marker, Ly110
We generated monoclonal antibodies reacting to a mouse thymic epithelial cell specific membrane protein, Thymic Stromal Co-transporter (TSCOT)/Ly110. These antibodies showed specificity to the peptide sequences derived from TSCOT/Ly110 determined by specific peptide inhibition in flow cytometric ana...
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| Veröffentlicht in: | Journal of immunological methods 2005-02, Vol.297 (1), p.265-270 |
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| creator | Yang, Soo Jung Ahn, Sejin Park, Chan-Sik Choi, Seeyoung Kim, Moon Gyo |
| description | We generated monoclonal antibodies reacting to a mouse thymic epithelial cell specific membrane protein, Thymic Stromal Co-transporter (TSCOT)/Ly110. These antibodies showed specificity to the peptide sequences derived from TSCOT/Ly110 determined by specific peptide inhibition in flow cytometric analyses with cells expressing the protein on the surface. TSCOT/Ly110 expressing subpopulation can be identified among the CDR1
+ or 6C3
+ cortical epithelial cells. Furthermore, CDR1 positive cortical thymic epithelial cells can be separated into further distinguishable populations; CDR1
+6C3
+Ly110
+, CDR1
+6C3
−/
lowLy110
+, CDR1
+Ly110
−. Some of TSCOT/Ly110 expressing cells negative for both CDR1 and 6C3 markers were found at the earlier stages of development, while most of the cells are positive for both at 1-week-old stage. After then, downregulation in 6C3 and/or CDR1 expression was noticed until 16 weeks of age. These results suggest that TSCOT/Ly110 is a new marker for the subpopulation of CDR1
+ or 6C3
+
epithelial cells in the neonatal and adult thymus and is useful for the studies on the epithelial cell differentiation process. |
| doi_str_mv | 10.1016/j.jim.2004.12.021 |
| format | Article |
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+ or 6C3
+ cortical epithelial cells. Furthermore, CDR1 positive cortical thymic epithelial cells can be separated into further distinguishable populations; CDR1
+6C3
+Ly110
+, CDR1
+6C3
−/
lowLy110
+, CDR1
+Ly110
−. Some of TSCOT/Ly110 expressing cells negative for both CDR1 and 6C3 markers were found at the earlier stages of development, while most of the cells are positive for both at 1-week-old stage. After then, downregulation in 6C3 and/or CDR1 expression was noticed until 16 weeks of age. These results suggest that TSCOT/Ly110 is a new marker for the subpopulation of CDR1
+ or 6C3
+
epithelial cells in the neonatal and adult thymus and is useful for the studies on the epithelial cell differentiation process.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2004.12.021</identifier><identifier>PMID: 15777949</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Biomarkers - analysis ; Cell Differentiation ; Epithelial Cells - classification ; Epithelial Cells - cytology ; Epithelial Cells - immunology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; mean fluorescence intensity ; MFI ; Mice ; Molecular immunology ; Nerve Tissue Proteins - analysis ; Nerve Tissue Proteins - immunology ; Specific marker ; Symporters - analysis ; Symporters - immunology ; TEC ; Techniques ; thymic epithelial cell ; Thymic epithelial cells ; Thymic Stroma Cotransporter ; Thymus Gland - cytology ; Thymus Gland - immunology ; TSCOT ; TSCOT/Ly110</subject><ispartof>Journal of immunological methods, 2005-02, Vol.297 (1), p.265-270</ispartof><rights>2005</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-66bd97c2dded93d7c11f7196e1ae3214a1c185011420969004911e64bbd4d91b3</citedby><cites>FETCH-LOGICAL-c358t-66bd97c2dded93d7c11f7196e1ae3214a1c185011420969004911e64bbd4d91b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175905000165$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16638201$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15777949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Soo Jung</creatorcontrib><creatorcontrib>Ahn, Sejin</creatorcontrib><creatorcontrib>Park, Chan-Sik</creatorcontrib><creatorcontrib>Choi, Seeyoung</creatorcontrib><creatorcontrib>Kim, Moon Gyo</creatorcontrib><title>Identifying subpopulations of thymic epithelial cells by flow cytometry using a new specific thymic epithelial marker, Ly110</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>We generated monoclonal antibodies reacting to a mouse thymic epithelial cell specific membrane protein, Thymic Stromal Co-transporter (TSCOT)/Ly110. These antibodies showed specificity to the peptide sequences derived from TSCOT/Ly110 determined by specific peptide inhibition in flow cytometric analyses with cells expressing the protein on the surface. TSCOT/Ly110 expressing subpopulation can be identified among the CDR1
+ or 6C3
+ cortical epithelial cells. Furthermore, CDR1 positive cortical thymic epithelial cells can be separated into further distinguishable populations; CDR1
+6C3
+Ly110
+, CDR1
+6C3
−/
lowLy110
+, CDR1
+Ly110
−. Some of TSCOT/Ly110 expressing cells negative for both CDR1 and 6C3 markers were found at the earlier stages of development, while most of the cells are positive for both at 1-week-old stage. After then, downregulation in 6C3 and/or CDR1 expression was noticed until 16 weeks of age. These results suggest that TSCOT/Ly110 is a new marker for the subpopulation of CDR1
+ or 6C3
+
epithelial cells in the neonatal and adult thymus and is useful for the studies on the epithelial cell differentiation process.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Cell Differentiation</subject><subject>Epithelial Cells - classification</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - immunology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>mean fluorescence intensity</subject><subject>MFI</subject><subject>Mice</subject><subject>Molecular immunology</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - immunology</subject><subject>Specific marker</subject><subject>Symporters - analysis</subject><subject>Symporters - immunology</subject><subject>TEC</subject><subject>Techniques</subject><subject>thymic epithelial cell</subject><subject>Thymic epithelial cells</subject><subject>Thymic Stroma Cotransporter</subject><subject>Thymus Gland - cytology</subject><subject>Thymus Gland - immunology</subject><subject>TSCOT</subject><subject>TSCOT/Ly110</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoTs_oD3Aj2ejKKu9NPdLBlQw6DjS40XVIJbectPUyqXIo8MebohtmITirbL5zuPkOY68QcgSs3x_zo-9zAVDmKHIQ-ITtcC9FJhVUT9kOQIgMZaUu2GWMRwBAqOE5u8BKSqlKtWN_bh0Ns29XP_zgcWmmcVo6M_txiHxs-Xy39t5ymvx8R503HbfUdZE3K2-78Z7bdR57msPKl7g1GD7QPY8TWd-m3L_x3oSfFN7xw4oIL9iz1nSRXp7fK_b986dv11-yw9eb2-uPh8wW1X7O6rpxSlrhHDlVOGkRW4mqJjRUCCwNWtxXgFgKULVKOhQi1WXTuNIpbIor9vbUO4Xx10Jx1r2P20fMQOMSdS2rIqmqHgUFSFlWCh4Fk_SirHAD8QTaMMYYqNVT8EnCqhH0NqI-6jSi3kbUKHQaMWVen8uXpif3kDivloA3Z8BEa7o2mMH6-MDVdbEXsBV9OHGU5P72FHS0ngZLzgeys3aj_88ZfwHL7LpF</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Yang, Soo Jung</creator><creator>Ahn, Sejin</creator><creator>Park, Chan-Sik</creator><creator>Choi, Seeyoung</creator><creator>Kim, Moon Gyo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Identifying subpopulations of thymic epithelial cells by flow cytometry using a new specific thymic epithelial marker, Ly110</title><author>Yang, Soo Jung ; Ahn, Sejin ; Park, Chan-Sik ; Choi, Seeyoung ; Kim, Moon Gyo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-66bd97c2dded93d7c11f7196e1ae3214a1c185011420969004911e64bbd4d91b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Cell Differentiation</topic><topic>Epithelial Cells - classification</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - immunology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>mean fluorescence intensity</topic><topic>MFI</topic><topic>Mice</topic><topic>Molecular immunology</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - immunology</topic><topic>Specific marker</topic><topic>Symporters - analysis</topic><topic>Symporters - immunology</topic><topic>TEC</topic><topic>Techniques</topic><topic>thymic epithelial cell</topic><topic>Thymic epithelial cells</topic><topic>Thymic Stroma Cotransporter</topic><topic>Thymus Gland - cytology</topic><topic>Thymus Gland - immunology</topic><topic>TSCOT</topic><topic>TSCOT/Ly110</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Soo Jung</creatorcontrib><creatorcontrib>Ahn, Sejin</creatorcontrib><creatorcontrib>Park, Chan-Sik</creatorcontrib><creatorcontrib>Choi, Seeyoung</creatorcontrib><creatorcontrib>Kim, Moon Gyo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Soo Jung</au><au>Ahn, Sejin</au><au>Park, Chan-Sik</au><au>Choi, Seeyoung</au><au>Kim, Moon Gyo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identifying subpopulations of thymic epithelial cells by flow cytometry using a new specific thymic epithelial marker, Ly110</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2005-02</date><risdate>2005</risdate><volume>297</volume><issue>1</issue><spage>265</spage><epage>270</epage><pages>265-270</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>We generated monoclonal antibodies reacting to a mouse thymic epithelial cell specific membrane protein, Thymic Stromal Co-transporter (TSCOT)/Ly110. These antibodies showed specificity to the peptide sequences derived from TSCOT/Ly110 determined by specific peptide inhibition in flow cytometric analyses with cells expressing the protein on the surface. TSCOT/Ly110 expressing subpopulation can be identified among the CDR1
+ or 6C3
+ cortical epithelial cells. Furthermore, CDR1 positive cortical thymic epithelial cells can be separated into further distinguishable populations; CDR1
+6C3
+Ly110
+, CDR1
+6C3
−/
lowLy110
+, CDR1
+Ly110
−. Some of TSCOT/Ly110 expressing cells negative for both CDR1 and 6C3 markers were found at the earlier stages of development, while most of the cells are positive for both at 1-week-old stage. After then, downregulation in 6C3 and/or CDR1 expression was noticed until 16 weeks of age. These results suggest that TSCOT/Ly110 is a new marker for the subpopulation of CDR1
+ or 6C3
+
epithelial cells in the neonatal and adult thymus and is useful for the studies on the epithelial cell differentiation process.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15777949</pmid><doi>10.1016/j.jim.2004.12.021</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal - immunology Biological and medical sciences Biomarkers - analysis Cell Differentiation Epithelial Cells - classification Epithelial Cells - cytology Epithelial Cells - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology mean fluorescence intensity MFI Mice Molecular immunology Nerve Tissue Proteins - analysis Nerve Tissue Proteins - immunology Specific marker Symporters - analysis Symporters - immunology TEC Techniques thymic epithelial cell Thymic epithelial cells Thymic Stroma Cotransporter Thymus Gland - cytology Thymus Gland - immunology TSCOT TSCOT/Ly110 |
| title | Identifying subpopulations of thymic epithelial cells by flow cytometry using a new specific thymic epithelial marker, Ly110 |
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