Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus
Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation...
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| Veröffentlicht in: | Journal of experimental botany 2005-04, Vol.56 (414), p.1245-1253 |
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| creator | Teixeira, Rita Teresa Knorpp, Carina Glimelius, Kristina |
| description | Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number of chloroplasts per cell area in CMS plants compared with B. napus. The increase in chloroplast density was found to be the result of the smaller mesophyll cells. Sucrose concentration in the leaves of the CMS lines was reduced both in green leaves as well as in leaves from 2 d-etiolated plants. Flower meristem, flower buds, and leaves from green and 2 d-etiolated plants were analysed for ATP and ADP contents. All CMS plant tissues, except for green leaves, possessed lower ATP levels than B. napus. The results indicate that the reduced availability of energy, i.e. ATP and sucrose in the CMS plants, limits plant growth. This is supported by the reduced levels of two D-type cyclin transcripts and the reduced capacity of the CMS plants to recover after etiolation. |
| doi_str_mv | 10.1093/jxb/eri120 |
| format | Article |
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Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number of chloroplasts per cell area in CMS plants compared with B. napus. The increase in chloroplast density was found to be the result of the smaller mesophyll cells. Sucrose concentration in the leaves of the CMS lines was reduced both in green leaves as well as in leaves from 2 d-etiolated plants. Flower meristem, flower buds, and leaves from green and 2 d-etiolated plants were analysed for ATP and ADP contents. All CMS plant tissues, except for green leaves, possessed lower ATP levels than B. napus. The results indicate that the reduced availability of energy, i.e. ATP and sucrose in the CMS plants, limits plant growth. This is supported by the reduced levels of two D-type cyclin transcripts and the reduced capacity of the CMS plants to recover after etiolation.</description><identifier>ISSN: 0022-0957</identifier><identifier>ISSN: 1460-2431</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/eri120</identifier><identifier>PMID: 15753110</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adenosine Diphosphate - metabolism ; adenosine triphosphate ; Adenosine Triphosphate - metabolism ; Arabidopsis - genetics ; ATP ; B. napus ; Biological and medical sciences ; Brassica napus ; Brassica napus - genetics ; Brassica napus - metabolism ; buds ; Cell lines ; cell nucleus ; Cell Size ; chemical constituents of plants ; Chloroplasts ; Chloroplasts - metabolism ; Chloroplasts - ultrastructure ; CMS ; cytoplasmic male sterility ; Darkness ; DNA Primers ; flower primordia ; flowers ; Flowers - metabolism ; Fundamental and applied biological sciences. Psychology ; Internodes ; Kinetics ; Leaves ; Meristem - metabolism ; mesophyll ; Mesophyll cells ; Metabolism ; mitochondria ; mitochondrial DNA ; Photosynthesis, respiration. Anabolism, catabolism ; Plant cells ; Plant growth ; Plant Leaves - metabolism ; Plant Leaves - ultrastructure ; Plant physiology and development ; Plants ; Plants, Genetically Modified - metabolism ; plastids ; Protoplasts ; rapeseed ; Reproduction ; RESEARCH PAPER ; Reverse Transcriptase Polymerase Chain Reaction ; starch ; Starch - metabolism ; Starches ; sucrose ; Sucrose - metabolism</subject><ispartof>Journal of experimental botany, 2005-04, Vol.56 (414), p.1245-1253</ispartof><rights>Society for Experimental Biology 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Apr 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-b38ed62e88d29ce51164f6026175e1b236e04c7c8db27bb85ee40e30557411943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24030922$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24030922$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16694466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15753110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teixeira, Rita Teresa</creatorcontrib><creatorcontrib>Knorpp, Carina</creatorcontrib><creatorcontrib>Glimelius, Kristina</creatorcontrib><title>Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus</title><title>Journal of experimental botany</title><addtitle>J. Exp. Bot</addtitle><description>Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number of chloroplasts per cell area in CMS plants compared with B. napus. The increase in chloroplast density was found to be the result of the smaller mesophyll cells. Sucrose concentration in the leaves of the CMS lines was reduced both in green leaves as well as in leaves from 2 d-etiolated plants. Flower meristem, flower buds, and leaves from green and 2 d-etiolated plants were analysed for ATP and ADP contents. All CMS plant tissues, except for green leaves, possessed lower ATP levels than B. napus. The results indicate that the reduced availability of energy, i.e. ATP and sucrose in the CMS plants, limits plant growth. This is supported by the reduced levels of two D-type cyclin transcripts and the reduced capacity of the CMS plants to recover after etiolation.</description><subject>Adenosine Diphosphate - metabolism</subject><subject>adenosine triphosphate</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Arabidopsis - genetics</subject><subject>ATP</subject><subject>B. napus</subject><subject>Biological and medical sciences</subject><subject>Brassica napus</subject><subject>Brassica napus - genetics</subject><subject>Brassica napus - metabolism</subject><subject>buds</subject><subject>Cell lines</subject><subject>cell nucleus</subject><subject>Cell Size</subject><subject>chemical constituents of plants</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Chloroplasts - ultrastructure</subject><subject>CMS</subject><subject>cytoplasmic male sterility</subject><subject>Darkness</subject><subject>DNA Primers</subject><subject>flower primordia</subject><subject>flowers</subject><subject>Flowers - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Internodes</subject><subject>Kinetics</subject><subject>Leaves</subject><subject>Meristem - metabolism</subject><subject>mesophyll</subject><subject>Mesophyll cells</subject><subject>Metabolism</subject><subject>mitochondria</subject><subject>mitochondrial DNA</subject><subject>Photosynthesis, respiration. Anabolism, catabolism</subject><subject>Plant cells</subject><subject>Plant growth</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - ultrastructure</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>plastids</subject><subject>Protoplasts</subject><subject>rapeseed</subject><subject>Reproduction</subject><subject>RESEARCH PAPER</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>starch</subject><subject>Starch - metabolism</subject><subject>Starches</subject><subject>sucrose</subject><subject>Sucrose - metabolism</subject><issn>0022-0957</issn><issn>1460-2431</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0E1v1DAQBmALgehSuHAHLKRyQE07_kx8bFdAkVpRRItQL5aTTCCLN17sBMq_x6usWomTD--jGc9LyHMGRwyMOF7d1scYe8bhAVkwqaHgUrCHZAHAeQFGlXvkSUorAFCg1GOyx1SpBGOwIDcXoe27HluapiaGhIc0jS42Pw6pG1p6cnVJPf5Gn2g_0PFPoM77sPEurfuGrp3HIo15t0fq-wETDR09PaKD20zpKXnUOZ_w2e7dJ9fv310tz4rzTx8-Lk_Oi0YaPha1qLDVHKuq5aZBxZiWnQauWamQ1VxoBNmUTdXWvKzrSiFKQJEPKSVjRop98maeu4nh14RptOs-Nei9GzBMyepScQNMZPj6P7gKUxzy3ywXKgumdEZvZ7RtI0Xs7Cb2axf_WgZ2W7fNddu57oxf7iZO9Rrbe7rrN4ODHXCpcb6Lbmj6dO-0NlLq7dYXs1ulMcS7nEsQYDjPeTHnfa779i538Wc-T5TKnn27sRdLUF_hUtjP2b-afeeCdd9j3nn9hecTAYzRII34B4Spp_Y</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Teixeira, Rita Teresa</creator><creator>Knorpp, Carina</creator><creator>Glimelius, Kristina</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus</title><author>Teixeira, Rita Teresa ; Knorpp, Carina ; Glimelius, Kristina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-b38ed62e88d29ce51164f6026175e1b236e04c7c8db27bb85ee40e30557411943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenosine Diphosphate - metabolism</topic><topic>adenosine triphosphate</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Arabidopsis - genetics</topic><topic>ATP</topic><topic>B. napus</topic><topic>Biological and medical sciences</topic><topic>Brassica napus</topic><topic>Brassica napus - genetics</topic><topic>Brassica napus - metabolism</topic><topic>buds</topic><topic>Cell lines</topic><topic>cell nucleus</topic><topic>Cell Size</topic><topic>chemical constituents of plants</topic><topic>Chloroplasts</topic><topic>Chloroplasts - metabolism</topic><topic>Chloroplasts - ultrastructure</topic><topic>CMS</topic><topic>cytoplasmic male sterility</topic><topic>Darkness</topic><topic>DNA Primers</topic><topic>flower primordia</topic><topic>flowers</topic><topic>Flowers - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Internodes</topic><topic>Kinetics</topic><topic>Leaves</topic><topic>Meristem - metabolism</topic><topic>mesophyll</topic><topic>Mesophyll cells</topic><topic>Metabolism</topic><topic>mitochondria</topic><topic>mitochondrial DNA</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Plant cells</topic><topic>Plant growth</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - ultrastructure</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>plastids</topic><topic>Protoplasts</topic><topic>rapeseed</topic><topic>Reproduction</topic><topic>RESEARCH PAPER</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>starch</topic><topic>Starch - metabolism</topic><topic>Starches</topic><topic>sucrose</topic><topic>Sucrose - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teixeira, Rita Teresa</creatorcontrib><creatorcontrib>Knorpp, Carina</creatorcontrib><creatorcontrib>Glimelius, Kristina</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, Rita Teresa</au><au>Knorpp, Carina</au><au>Glimelius, Kristina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J. Exp. Bot</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>56</volume><issue>414</issue><spage>1245</spage><epage>1253</epage><pages>1245-1253</pages><issn>0022-0957</issn><issn>1460-2431</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number of chloroplasts per cell area in CMS plants compared with B. napus. The increase in chloroplast density was found to be the result of the smaller mesophyll cells. Sucrose concentration in the leaves of the CMS lines was reduced both in green leaves as well as in leaves from 2 d-etiolated plants. Flower meristem, flower buds, and leaves from green and 2 d-etiolated plants were analysed for ATP and ADP contents. All CMS plant tissues, except for green leaves, possessed lower ATP levels than B. napus. The results indicate that the reduced availability of energy, i.e. ATP and sucrose in the CMS plants, limits plant growth. This is supported by the reduced levels of two D-type cyclin transcripts and the reduced capacity of the CMS plants to recover after etiolation.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15753110</pmid><doi>10.1093/jxb/eri120</doi><tpages>9</tpages></addata></record> |
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| subjects | Adenosine Diphosphate - metabolism adenosine triphosphate Adenosine Triphosphate - metabolism Arabidopsis - genetics ATP B. napus Biological and medical sciences Brassica napus Brassica napus - genetics Brassica napus - metabolism buds Cell lines cell nucleus Cell Size chemical constituents of plants Chloroplasts Chloroplasts - metabolism Chloroplasts - ultrastructure CMS cytoplasmic male sterility Darkness DNA Primers flower primordia flowers Flowers - metabolism Fundamental and applied biological sciences. Psychology Internodes Kinetics Leaves Meristem - metabolism mesophyll Mesophyll cells Metabolism mitochondria mitochondrial DNA Photosynthesis, respiration. Anabolism, catabolism Plant cells Plant growth Plant Leaves - metabolism Plant Leaves - ultrastructure Plant physiology and development Plants Plants, Genetically Modified - metabolism plastids Protoplasts rapeseed Reproduction RESEARCH PAPER Reverse Transcriptase Polymerase Chain Reaction starch Starch - metabolism Starches sucrose Sucrose - metabolism |
| title | Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus |
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