Development of a High-Density Assay for Long-Chain Fatty Acyl-CoA Elongases

We established a convenient assay method for measuring elongation of very long chain fatty acids (ELOVLs) using a Unifilter-96 GF/C plate. The Unifilter GF/C plate preferentially interacts with hydrophobic end products of ELOVLs (i.e., long chain fatty acid), with minimal malonyl-CoA (C2 unit donor...

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Veröffentlicht in:	Lipids 2009-08, Vol.44 (8), p.765-773
Hauptverfasser:	Kitazawa, Hidefumi, Miyamoto, Yasuhisa, Shimamura, Ken, Nagumo, Akira, Tokita, Shigeru
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyltransferases - analysis Acetyltransferases - genetics Acetyltransferases - metabolism Acyl Coenzyme A - metabolism acyltransferases Animals Biomedical and Life Sciences Cells, Cultured Cercopithecus aethiops complementary DNA COS Cells Diabetes Elongase ELOVL enzyme activity fatty acid elongases Fatty acids gene expression regulation human diseases human health Humans Immunoradiometric Assay - methods Life Sciences lipid metabolism Lipidology Long chain fatty acid long chain fatty acids Long chain fatty acyl‐CoA Malonyl Coenzyme A - metabolism Malonyl‐CoA Medical Biochemistry Medicinal Chemistry Metabolic disorder Methods Microbial Genetics and Genomics microsomes Microsomes - metabolism molecular cloning Neurochemistry noninsulin-dependent diabetes mellitus Nutrition Obesity Palmitoyl Coenzyme A - metabolism Protein Binding recombinant fusion proteins Substrate Specificity Transfection
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