Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)

To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. Genomic DNA was isolated and PCR was set up by using specific primers. Am...

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Veröffentlicht in:	Letters in applied microbiology 2009-08, Vol.49 (2), p.274-277
Hauptverfasser:	Chaudhary, P.P, Sirohi, S.K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biodiversity Biological and medical sciences Bubalus bubalis Buffaloes - microbiology Cluster Analysis DNA, Archaeal - chemistry DNA, Archaeal - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Female Fundamental and applied biological sciences. Psychology Genes, rRNA India Male Methane - metabolism methanogens Methanomicrobiaceae - classification Methanomicrobiaceae - genetics Methanomicrobiaceae - isolation & purification Methanomicrobiaceae - metabolism Methanomicrobium Methanomicrobium mobile Microbiology Molecular Sequence Data Murrah buffaloes Phylogeny phylotype RNA, Archaeal - genetics RNA, Ribosomal, 16S - genetics Rumen - microbiology Sequence Analysis, DNA Sequence Homology, Nucleic Acid
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