Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)
To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. Genomic DNA was isolated and PCR was set up by using specific primers. Am...
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| Veröffentlicht in: | Letters in applied microbiology 2009-08, Vol.49 (2), p.274-277 |
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| description | To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. Genomic DNA was isolated and PCR was set up by using specific primers. Amplified product was cloned into a suitable vector and the positive clones were selected on the basis of blue-white screening and sequenced. The resulting nucleotide sequences were arranged in the phylogenetic tree. A total of 108 clones were examined, revealing 17 different 16S rRNA gene sequences or phylotypes. Of the 17 phylotypes, 15 (102 of 108 clones) belonged to the genus Methanomicrobium, indicating that the genus Methanomicrobium is the most dominant component of methanogen populations in Murrah buffaloes (Bubalus bubalis) from North India. The largest group of clones (102 clones) was more than 98% similar to Methanomicrobium mobile. BLAST analysis of the rumen contents from individual animals also revealed 17 different phylotypes with a range of 3-10 phylotypes per animal. Methanomicrobium phylotype is the most dominant phylotype of methanogens present in Murrah buffaloes (Bubalus bubalis). Effective strategies can be made to inhibit the growth of Methanomicrobium phylotype to reduce the methane emission from rumen contents and thus help in preventing global warming. |
| doi_str_mv | 10.1111/j.1472-765X.2009.02654.x |
| format | Article |
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Genomic DNA was isolated and PCR was set up by using specific primers. Amplified product was cloned into a suitable vector and the positive clones were selected on the basis of blue-white screening and sequenced. The resulting nucleotide sequences were arranged in the phylogenetic tree. A total of 108 clones were examined, revealing 17 different 16S rRNA gene sequences or phylotypes. Of the 17 phylotypes, 15 (102 of 108 clones) belonged to the genus Methanomicrobium, indicating that the genus Methanomicrobium is the most dominant component of methanogen populations in Murrah buffaloes (Bubalus bubalis) from North India. The largest group of clones (102 clones) was more than 98% similar to Methanomicrobium mobile. BLAST analysis of the rumen contents from individual animals also revealed 17 different phylotypes with a range of 3-10 phylotypes per animal. Methanomicrobium phylotype is the most dominant phylotype of methanogens present in Murrah buffaloes (Bubalus bubalis). Effective strategies can be made to inhibit the growth of Methanomicrobium phylotype to reduce the methane emission from rumen contents and thus help in preventing global warming.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2009.02654.x</identifier><identifier>PMID: 19486281</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Biodiversity ; Biological and medical sciences ; Bubalus bubalis ; Buffaloes - microbiology ; Cluster Analysis ; DNA, Archaeal - chemistry ; DNA, Archaeal - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; India ; Male ; Methane - metabolism ; methanogens ; Methanomicrobiaceae - classification ; Methanomicrobiaceae - genetics ; Methanomicrobiaceae - isolation & purification ; Methanomicrobiaceae - metabolism ; Methanomicrobium ; Methanomicrobium mobile ; Microbiology ; Molecular Sequence Data ; Murrah buffaloes ; Phylogeny ; phylotype ; RNA, Archaeal - genetics ; RNA, Ribosomal, 16S - genetics ; Rumen - microbiology ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid</subject><ispartof>Letters in applied microbiology, 2009-08, Vol.49 (2), p.274-277</ispartof><rights>2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4524-ac5537b1494c0dbb3a1b98da68a447b88e76796292a3180815c3c5fed1e7cbff3</citedby><cites>FETCH-LOGICAL-c4524-ac5537b1494c0dbb3a1b98da68a447b88e76796292a3180815c3c5fed1e7cbff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2009.02654.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2009.02654.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21724872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19486281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaudhary, P.P</creatorcontrib><creatorcontrib>Sirohi, S.K</creatorcontrib><title>Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. Genomic DNA was isolated and PCR was set up by using specific primers. Amplified product was cloned into a suitable vector and the positive clones were selected on the basis of blue-white screening and sequenced. The resulting nucleotide sequences were arranged in the phylogenetic tree. A total of 108 clones were examined, revealing 17 different 16S rRNA gene sequences or phylotypes. Of the 17 phylotypes, 15 (102 of 108 clones) belonged to the genus Methanomicrobium, indicating that the genus Methanomicrobium is the most dominant component of methanogen populations in Murrah buffaloes (Bubalus bubalis) from North India. The largest group of clones (102 clones) was more than 98% similar to Methanomicrobium mobile. BLAST analysis of the rumen contents from individual animals also revealed 17 different phylotypes with a range of 3-10 phylotypes per animal. Methanomicrobium phylotype is the most dominant phylotype of methanogens present in Murrah buffaloes (Bubalus bubalis). Effective strategies can be made to inhibit the growth of Methanomicrobium phylotype to reduce the methane emission from rumen contents and thus help in preventing global warming.</description><subject>Animals</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Bubalus bubalis</subject><subject>Buffaloes - microbiology</subject><subject>Cluster Analysis</subject><subject>DNA, Archaeal - chemistry</subject><subject>DNA, Archaeal - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>India</subject><subject>Male</subject><subject>Methane - metabolism</subject><subject>methanogens</subject><subject>Methanomicrobiaceae - classification</subject><subject>Methanomicrobiaceae - genetics</subject><subject>Methanomicrobiaceae - isolation & purification</subject><subject>Methanomicrobiaceae - metabolism</subject><subject>Methanomicrobium</subject><subject>Methanomicrobium mobile</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Murrah buffaloes</subject><subject>Phylogeny</subject><subject>phylotype</subject><subject>RNA, Archaeal - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Rumen - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCXwBfQOWQ4G87Bw6l0BZpVxygEjfL9jrdrJI4tRPR_fc4ZFWO1JcZzfuMx54XAIhRifP5uC8xk6SQgv8qCUJViYjgrHx4BlaPwnOwylVRKMLZCThNaY8QUphUL8EJrpgSROEV6L-ErulN7zwMNdz4cWf6XHEx2Gbq4LA7tGE8DB42PewW9c73cAjD1JqxCTmNPvl-nIHNFKPZQTvVtWmDT_D882RNO6VcyrFJH16BF1lK_vUxnoHbq68_L2-K9ffrb5cX68IxTlhhHOdUWswq5tDWWmqwrdTWCGUYk1YpL4WsBKmIoVjlX3FHHa_9FnvpbF3TM_B-uXeI4X7yadRdk5xvW9P7MCUtJCeEMvVfkCCCKKM0g2oB82pSir7WQ2w6Ew8aIz2bovd63r2ed69nU_RfU_RDbn1znDHZzm__NR5dyMC7I2CSM20dsx9NeuQIloQpSTL3aeF-N60_PPkBen2xmbPc_3bpr03Q5i7mGbc_CMIU4SwzJugfdvSy9g</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Chaudhary, P.P</creator><creator>Sirohi, S.K</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)</title><author>Chaudhary, P.P ; Sirohi, S.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4524-ac5537b1494c0dbb3a1b98da68a447b88e76796292a3180815c3c5fed1e7cbff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Bubalus bubalis</topic><topic>Buffaloes - microbiology</topic><topic>Cluster Analysis</topic><topic>DNA, Archaeal - chemistry</topic><topic>DNA, Archaeal - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>India</topic><topic>Male</topic><topic>Methane - metabolism</topic><topic>methanogens</topic><topic>Methanomicrobiaceae - classification</topic><topic>Methanomicrobiaceae - genetics</topic><topic>Methanomicrobiaceae - isolation & purification</topic><topic>Methanomicrobiaceae - metabolism</topic><topic>Methanomicrobium</topic><topic>Methanomicrobium mobile</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Murrah buffaloes</topic><topic>Phylogeny</topic><topic>phylotype</topic><topic>RNA, Archaeal - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Rumen - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaudhary, P.P</creatorcontrib><creatorcontrib>Sirohi, S.K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaudhary, P.P</au><au>Sirohi, S.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis)</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2009-08</date><risdate>2009</risdate><volume>49</volume><issue>2</issue><spage>274</spage><epage>277</epage><pages>274-277</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. Genomic DNA was isolated and PCR was set up by using specific primers. Amplified product was cloned into a suitable vector and the positive clones were selected on the basis of blue-white screening and sequenced. The resulting nucleotide sequences were arranged in the phylogenetic tree. A total of 108 clones were examined, revealing 17 different 16S rRNA gene sequences or phylotypes. Of the 17 phylotypes, 15 (102 of 108 clones) belonged to the genus Methanomicrobium, indicating that the genus Methanomicrobium is the most dominant component of methanogen populations in Murrah buffaloes (Bubalus bubalis) from North India. The largest group of clones (102 clones) was more than 98% similar to Methanomicrobium mobile. BLAST analysis of the rumen contents from individual animals also revealed 17 different phylotypes with a range of 3-10 phylotypes per animal. Methanomicrobium phylotype is the most dominant phylotype of methanogens present in Murrah buffaloes (Bubalus bubalis). Effective strategies can be made to inhibit the growth of Methanomicrobium phylotype to reduce the methane emission from rumen contents and thus help in preventing global warming.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19486281</pmid><doi>10.1111/j.1472-765X.2009.02654.x</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0266-8254 |
| ispartof | Letters in applied microbiology, 2009-08, Vol.49 (2), p.274-277 |
| issn | 0266-8254 1472-765X |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Biodiversity Biological and medical sciences Bubalus bubalis Buffaloes - microbiology Cluster Analysis DNA, Archaeal - chemistry DNA, Archaeal - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Female Fundamental and applied biological sciences. Psychology Genes, rRNA India Male Methane - metabolism methanogens Methanomicrobiaceae - classification Methanomicrobiaceae - genetics Methanomicrobiaceae - isolation & purification Methanomicrobiaceae - metabolism Methanomicrobium Methanomicrobium mobile Microbiology Molecular Sequence Data Murrah buffaloes Phylogeny phylotype RNA, Archaeal - genetics RNA, Ribosomal, 16S - genetics Rumen - microbiology Sequence Analysis, DNA Sequence Homology, Nucleic Acid |
| title | Dominance of Methanomicrobium phylotype in methanogen population present in Murrah buffaloes (Bubalus bubalis) |
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