Molecular cloning and functional expression of hemolysin from the sea anemone Actineria villosa

The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681 bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypept...

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Veröffentlicht in:	Protein expression and purification 2005-04, Vol.40 (2), p.379-384
Hauptverfasser:	Uechi, Gen-ichiro, Toma, Hiromu, Arakawa, Takeshi, Sato, Yoshiya
Format:	Artikel
Sprache:	eng
Schlagworte:	Actineria villosa Animals Base Sequence Cloning, Molecular - methods DNA, Complementary - genetics Erythrocytes - drug effects Escherichia coli - genetics Hemolysin Hemolysin Proteins - chemistry Hemolysin Proteins - genetics Hemolysin Proteins - pharmacology Isoelectric Point M9Y medium Molecular Weight Recombinant Avt-I Recombinant Proteins - genetics Sea anemone Sea Anemones - chemistry Sequence Homology Sheep
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