An alternative use of basic pGEX vectors for producing both N- and C-terminal fusion proteins for production and affinity purification of antibodies

Glutathione S-transferase (GST) fusion proteins are widely used in protein production for pure immunogens, protein–protein, and DNA–protein interaction studies. Using basic pGEX vectors, foreign DNA is introduced to the C-terminus of the GST gene and the produced fusion proteins are C-terminally ori...

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Veröffentlicht in:	Protein expression and purification 2005-04, Vol.40 (2), p.287-291
Hauptverfasser:	Aatsinki, Jyrki T., Rajaniemi, Hannu J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity purification Antibodies - genetics Antibodies - isolation & purification Antibody production Chromatography, Affinity Cloning, Molecular - methods Electrophoresis, Polyacrylamide Gel Fusion protein Genetic Vectors Glutathione S-transferase Glutathione Transferase - genetics Luteinizing hormone/chorionic gonadotropin receptor Receptors, LH - immunology Recombinant Fusion Proteins Solubility
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