The Histology of Graft Adhesion in Descemet Stripping with Endothelial Keratoplasty
Purpose To describe the histologic findings of full-thickness corneas from penetrating keratoplasty (PK) in 10 patients with a prior history of Descemet stripping with endothelial keratoplasty. Design Retrospective review of the histopathology of 10 PK specimens. Methods We reviewed histologic secti...
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| Veröffentlicht in: | American journal of ophthalmology 2009-08, Vol.148 (2), p.277-281 |
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| creator | Caldwell, Matthew C Afshari, Natalie A Decroos, Francis C Proia, Alan D |
| description | Purpose To describe the histologic findings of full-thickness corneas from penetrating keratoplasty (PK) in 10 patients with a prior history of Descemet stripping with endothelial keratoplasty. Design Retrospective review of the histopathology of 10 PK specimens. Methods We reviewed histologic sections stained with hematoxylin and eosin, periodic acid-Schiff, and colloidal iron stains from 10 PK specimens, which had been trisected and submitted in their entirety. Clinical data were abstracted from the medical record. Results The interface between host and graft in most cases was barely perceptible with minimal changes in the degree of tissue eosinophilia, a subtle increase in keratocyte cellularity focally, or the presence of melanin granules within keratocytes. Residual host Descemet membrane (DM) was found in 8 of the 10 cases, most often at the edges of the graft. In no case did the presence of DM appear to hinder graft adhesion. Conclusion The adhesion of Descemet stripping with endothelial keratoplasty grafts is not associated with significant scarring or keratocyte proliferation. Contrary to previous assumptions, retained DM did not appear to hinder graft adhesion, raising the possibility that removal of DM may be unnecessary for endothelial transplantation. |
| doi_str_mv | 10.1016/j.ajo.2009.03.025 |
| format | Article |
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Design Retrospective review of the histopathology of 10 PK specimens. Methods We reviewed histologic sections stained with hematoxylin and eosin, periodic acid-Schiff, and colloidal iron stains from 10 PK specimens, which had been trisected and submitted in their entirety. Clinical data were abstracted from the medical record. Results The interface between host and graft in most cases was barely perceptible with minimal changes in the degree of tissue eosinophilia, a subtle increase in keratocyte cellularity focally, or the presence of melanin granules within keratocytes. Residual host Descemet membrane (DM) was found in 8 of the 10 cases, most often at the edges of the graft. In no case did the presence of DM appear to hinder graft adhesion. Conclusion The adhesion of Descemet stripping with endothelial keratoplasty grafts is not associated with significant scarring or keratocyte proliferation. Contrary to previous assumptions, retained DM did not appear to hinder graft adhesion, raising the possibility that removal of DM may be unnecessary for endothelial transplantation.</description><identifier>ISSN: 0002-9394</identifier><identifier>EISSN: 1879-1891</identifier><identifier>DOI: 10.1016/j.ajo.2009.03.025</identifier><identifier>PMID: 19464669</identifier><identifier>CODEN: AJOPAA</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Cell Adhesion ; Corneal Diseases - pathology ; Corneal Diseases - surgery ; Corneal Transplantation ; Descemet Membrane - pathology ; Descemet Membrane - surgery ; Endothelium, Corneal - pathology ; Endothelium, Corneal - transplantation ; Female ; Glaucoma ; Graft Survival ; Histology ; Humans ; Keratoplasty, Penetrating ; Male ; Medical sciences ; Middle Aged ; Miscellaneous ; Ophthalmology ; Patients ; Retrospective Studies ; Surgery ; Tissue Donors ; Wound Healing ; Young Adult</subject><ispartof>American journal of ophthalmology, 2009-08, Vol.148 (2), p.277-281</ispartof><rights>2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Elsevier Limited Aug 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-acc62c3defe09b37380516dd9c5707db1ca9b155aa392455a83fc1eeab2613173</citedby><cites>FETCH-LOGICAL-c464t-acc62c3defe09b37380516dd9c5707db1ca9b155aa392455a83fc1eeab2613173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ajo.2009.03.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21797662$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19464669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caldwell, Matthew C</creatorcontrib><creatorcontrib>Afshari, Natalie A</creatorcontrib><creatorcontrib>Decroos, Francis C</creatorcontrib><creatorcontrib>Proia, Alan D</creatorcontrib><title>The Histology of Graft Adhesion in Descemet Stripping with Endothelial Keratoplasty</title><title>American journal of ophthalmology</title><addtitle>Am J Ophthalmol</addtitle><description>Purpose To describe the histologic findings of full-thickness corneas from penetrating keratoplasty (PK) in 10 patients with a prior history of Descemet stripping with endothelial keratoplasty. Design Retrospective review of the histopathology of 10 PK specimens. Methods We reviewed histologic sections stained with hematoxylin and eosin, periodic acid-Schiff, and colloidal iron stains from 10 PK specimens, which had been trisected and submitted in their entirety. Clinical data were abstracted from the medical record. Results The interface between host and graft in most cases was barely perceptible with minimal changes in the degree of tissue eosinophilia, a subtle increase in keratocyte cellularity focally, or the presence of melanin granules within keratocytes. Residual host Descemet membrane (DM) was found in 8 of the 10 cases, most often at the edges of the graft. In no case did the presence of DM appear to hinder graft adhesion. Conclusion The adhesion of Descemet stripping with endothelial keratoplasty grafts is not associated with significant scarring or keratocyte proliferation. Contrary to previous assumptions, retained DM did not appear to hinder graft adhesion, raising the possibility that removal of DM may be unnecessary for endothelial transplantation.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Corneal Diseases - pathology</subject><subject>Corneal Diseases - surgery</subject><subject>Corneal Transplantation</subject><subject>Descemet Membrane - pathology</subject><subject>Descemet Membrane - surgery</subject><subject>Endothelium, Corneal - pathology</subject><subject>Endothelium, Corneal - transplantation</subject><subject>Female</subject><subject>Glaucoma</subject><subject>Graft Survival</subject><subject>Histology</subject><subject>Humans</subject><subject>Keratoplasty, Penetrating</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Ophthalmology</subject><subject>Patients</subject><subject>Retrospective Studies</subject><subject>Surgery</subject><subject>Tissue Donors</subject><subject>Wound Healing</subject><subject>Young Adult</subject><issn>0002-9394</issn><issn>1879-1891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ktFrFDEQxhdR7Fn9A3yRgNi3XSfJbnJBEEpbW7Hgw1XwLWSzs72se5ttklPuvzfHHRb64NMk8PtmvvmYonhLoaJAxcehMoOvGICqgFfAmmfFgi6lKulS0efFAgBYqbiqT4pXMQ75K2QtXxYnVNWiFkItitXdGsmNi8mP_n5HfE-ug-kTOe_WGJ2fiJvIJUaLG0xklYKbZzfdkz8urcnV1Pm0xtGZkXzDYJKfRxPT7nXxojdjxDfHelr8-HJ1d3FT3n6__npxflvaPD2VxlrBLO-wR1Atl3wJDRVdp2wjQXYttUa1tGmM4YrVuS55bymiaZmgnEp-Wpwd-s7BP2wxJr1x2ek4mgn9NmohG2ig5hl8_wQc_DZM2Zumom4A6kbUmaIHygYfY8Bez8FtTNhpCnqftx50zlvv89bAdc47a94dO2_bDXaPimPAGfhwBEy0ZuyDmayL_zhGpZJCsMx9OnCYA_vtMOhoHU4WOxfQJt15918bn5-o7egmlwf-wh3Gx211ZBr0an8Y-7sAlR9M_uR_AW_ksZ8</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Caldwell, Matthew C</creator><creator>Afshari, Natalie A</creator><creator>Decroos, Francis C</creator><creator>Proia, Alan D</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>The Histology of Graft Adhesion in Descemet Stripping with Endothelial Keratoplasty</title><author>Caldwell, Matthew C ; Afshari, Natalie A ; Decroos, Francis C ; Proia, Alan D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-acc62c3defe09b37380516dd9c5707db1ca9b155aa392455a83fc1eeab2613173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Corneal Diseases - pathology</topic><topic>Corneal Diseases - surgery</topic><topic>Corneal Transplantation</topic><topic>Descemet Membrane - pathology</topic><topic>Descemet Membrane - surgery</topic><topic>Endothelium, Corneal - pathology</topic><topic>Endothelium, Corneal - transplantation</topic><topic>Female</topic><topic>Glaucoma</topic><topic>Graft Survival</topic><topic>Histology</topic><topic>Humans</topic><topic>Keratoplasty, Penetrating</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Ophthalmology</topic><topic>Patients</topic><topic>Retrospective Studies</topic><topic>Surgery</topic><topic>Tissue Donors</topic><topic>Wound Healing</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caldwell, Matthew C</creatorcontrib><creatorcontrib>Afshari, Natalie A</creatorcontrib><creatorcontrib>Decroos, Francis C</creatorcontrib><creatorcontrib>Proia, Alan D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caldwell, Matthew C</au><au>Afshari, Natalie A</au><au>Decroos, Francis C</au><au>Proia, Alan D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Histology of Graft Adhesion in Descemet Stripping with Endothelial Keratoplasty</atitle><jtitle>American journal of ophthalmology</jtitle><addtitle>Am J Ophthalmol</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>148</volume><issue>2</issue><spage>277</spage><epage>281</epage><pages>277-281</pages><issn>0002-9394</issn><eissn>1879-1891</eissn><coden>AJOPAA</coden><abstract>Purpose To describe the histologic findings of full-thickness corneas from penetrating keratoplasty (PK) in 10 patients with a prior history of Descemet stripping with endothelial keratoplasty. Design Retrospective review of the histopathology of 10 PK specimens. Methods We reviewed histologic sections stained with hematoxylin and eosin, periodic acid-Schiff, and colloidal iron stains from 10 PK specimens, which had been trisected and submitted in their entirety. Clinical data were abstracted from the medical record. Results The interface between host and graft in most cases was barely perceptible with minimal changes in the degree of tissue eosinophilia, a subtle increase in keratocyte cellularity focally, or the presence of melanin granules within keratocytes. Residual host Descemet membrane (DM) was found in 8 of the 10 cases, most often at the edges of the graft. In no case did the presence of DM appear to hinder graft adhesion. Conclusion The adhesion of Descemet stripping with endothelial keratoplasty grafts is not associated with significant scarring or keratocyte proliferation. Contrary to previous assumptions, retained DM did not appear to hinder graft adhesion, raising the possibility that removal of DM may be unnecessary for endothelial transplantation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19464669</pmid><doi>10.1016/j.ajo.2009.03.025</doi><tpages>5</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Biological and medical sciences Cell Adhesion Corneal Diseases - pathology Corneal Diseases - surgery Corneal Transplantation Descemet Membrane - pathology Descemet Membrane - surgery Endothelium, Corneal - pathology Endothelium, Corneal - transplantation Female Glaucoma Graft Survival Histology Humans Keratoplasty, Penetrating Male Medical sciences Middle Aged Miscellaneous Ophthalmology Patients Retrospective Studies Surgery Tissue Donors Wound Healing Young Adult |
| title | The Histology of Graft Adhesion in Descemet Stripping with Endothelial Keratoplasty |
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