p15 mRNA expression detected by real-time quantitative reverse transcriptase-polymerase chain reaction correlates with the methylation density of the gene in adult acute leukemia
Cyclin-dependent kinase inhibitor p15 is frequently inactivated by either methylation or deletion in patients with acute leukemia. To examine pathologic and clinical significance of the p15 gene inactivation, we established a quantitative assay of p15 mRNA expression in the bone marrow cells by real...
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| Veröffentlicht in: | Leukemia research 2005-05, Vol.29 (5), p.557-564 |
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| creator | Matsuno, Naofumi Hoshino, Koyu Nanri, Tomoko Kawakita, Toshiro Suzushima, Hitoshi Kawano, Fumio Mitsuya, Hiroaki Asou, Norio |
| description | Cyclin-dependent kinase inhibitor
p15 is frequently inactivated by either methylation or deletion in patients with acute leukemia. To examine pathologic and clinical significance of the
p15 gene inactivation, we established a quantitative assay of
p15 mRNA expression in the bone marrow cells by real-time quantitative reverse transcriptase-polymerase chain reaction.
p15 mRNA expression in 14 patients with precursor B-cell acute lymphoblastic leukemia (PBC-ALL) well correlated with status of deletion and methylation in the
p15 gene analyzed by Southern blotting. Furthermore, two patients with PBC-ALL and 11 acute myeloblastic leukemia (AML) were quantitatively examined for
p15 gene methylation using bisulfite genomic sequencing. The data showed that
p15 mRNA expression significantly correlated with the CpG island methylation density. Among 108 AML patients,
p15 mRNA expression was significantly lower in the myeloid lineage (M1, M2, M3) than the monocytic lineage (M4, M5) (
P
=
0.0019). Above all, the majority of M3 patients showed low
p15 expression compared with M1 and M2 patients (
P
=
0.029). These observations suggest that quantitative analysis of
p15 mRNA will be useful to evaluate transcriptional repression of the
p15 gene caused by various degrees of methylation. |
| doi_str_mv | 10.1016/j.leukres.2004.11.003 |
| format | Article |
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p15 is frequently inactivated by either methylation or deletion in patients with acute leukemia. To examine pathologic and clinical significance of the
p15 gene inactivation, we established a quantitative assay of
p15 mRNA expression in the bone marrow cells by real-time quantitative reverse transcriptase-polymerase chain reaction.
p15 mRNA expression in 14 patients with precursor B-cell acute lymphoblastic leukemia (PBC-ALL) well correlated with status of deletion and methylation in the
p15 gene analyzed by Southern blotting. Furthermore, two patients with PBC-ALL and 11 acute myeloblastic leukemia (AML) were quantitatively examined for
p15 gene methylation using bisulfite genomic sequencing. The data showed that
p15 mRNA expression significantly correlated with the CpG island methylation density. Among 108 AML patients,
p15 mRNA expression was significantly lower in the myeloid lineage (M1, M2, M3) than the monocytic lineage (M4, M5) (
P
=
0.0019). Above all, the majority of M3 patients showed low
p15 expression compared with M1 and M2 patients (
P
=
0.029). These observations suggest that quantitative analysis of
p15 mRNA will be useful to evaluate transcriptional repression of the
p15 gene caused by various degrees of methylation.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/j.leukres.2004.11.003</identifier><identifier>PMID: 15755508</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acute Disease ; Adult ; ALL ; AML ; Bone Marrow Cells - metabolism ; Bone Marrow Cells - pathology ; Burkitt Lymphoma - genetics ; Burkitt Lymphoma - metabolism ; Burkitt Lymphoma - pathology ; Cell Cycle Proteins - genetics ; Cell Lineage ; CpG Islands ; Cyclin-Dependent Kinase Inhibitor p15 ; DNA Methylation ; Gene Expression Regulation, Leukemic ; Humans ; Leukemia, Myeloid - classification ; Leukemia, Myeloid - genetics ; Leukemia, Myeloid - metabolism ; Methylation ; p15 ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - metabolism ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; RQ-PCR ; Sequence Deletion ; Tumor Cells, Cultured ; Tumor Suppressor Proteins - genetics</subject><ispartof>Leukemia research, 2005-05, Vol.29 (5), p.557-564</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-a8fb6153d83a41b374a8852856b18c4a52e1c92c6599f215ac804decda89ea553</citedby><cites>FETCH-LOGICAL-c394t-a8fb6153d83a41b374a8852856b18c4a52e1c92c6599f215ac804decda89ea553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.leukres.2004.11.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15755508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuno, Naofumi</creatorcontrib><creatorcontrib>Hoshino, Koyu</creatorcontrib><creatorcontrib>Nanri, Tomoko</creatorcontrib><creatorcontrib>Kawakita, Toshiro</creatorcontrib><creatorcontrib>Suzushima, Hitoshi</creatorcontrib><creatorcontrib>Kawano, Fumio</creatorcontrib><creatorcontrib>Mitsuya, Hiroaki</creatorcontrib><creatorcontrib>Asou, Norio</creatorcontrib><title>p15 mRNA expression detected by real-time quantitative reverse transcriptase-polymerase chain reaction correlates with the methylation density of the gene in adult acute leukemia</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>Cyclin-dependent kinase inhibitor
p15 is frequently inactivated by either methylation or deletion in patients with acute leukemia. To examine pathologic and clinical significance of the
p15 gene inactivation, we established a quantitative assay of
p15 mRNA expression in the bone marrow cells by real-time quantitative reverse transcriptase-polymerase chain reaction.
p15 mRNA expression in 14 patients with precursor B-cell acute lymphoblastic leukemia (PBC-ALL) well correlated with status of deletion and methylation in the
p15 gene analyzed by Southern blotting. Furthermore, two patients with PBC-ALL and 11 acute myeloblastic leukemia (AML) were quantitatively examined for
p15 gene methylation using bisulfite genomic sequencing. The data showed that
p15 mRNA expression significantly correlated with the CpG island methylation density. Among 108 AML patients,
p15 mRNA expression was significantly lower in the myeloid lineage (M1, M2, M3) than the monocytic lineage (M4, M5) (
P
=
0.0019). Above all, the majority of M3 patients showed low
p15 expression compared with M1 and M2 patients (
P
=
0.029). These observations suggest that quantitative analysis of
p15 mRNA will be useful to evaluate transcriptional repression of the
p15 gene caused by various degrees of methylation.</description><subject>Acute Disease</subject><subject>Adult</subject><subject>ALL</subject><subject>AML</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Cells - pathology</subject><subject>Burkitt Lymphoma - genetics</subject><subject>Burkitt Lymphoma - metabolism</subject><subject>Burkitt Lymphoma - pathology</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Lineage</subject><subject>CpG Islands</subject><subject>Cyclin-Dependent Kinase Inhibitor p15</subject><subject>DNA Methylation</subject><subject>Gene Expression Regulation, Leukemic</subject><subject>Humans</subject><subject>Leukemia, Myeloid - classification</subject><subject>Leukemia, Myeloid - genetics</subject><subject>Leukemia, Myeloid - metabolism</subject><subject>Methylation</subject><subject>p15</subject><subject>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics</subject><subject>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - metabolism</subject><subject>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>RQ-PCR</subject><subject>Sequence Deletion</subject><subject>Tumor Cells, Cultured</subject><subject>Tumor Suppressor Proteins - genetics</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcmO1DAQhi0EYpqBRwD5xC3BFceJc0KjEZs0AgnB2ao41bSbbGM7DXktnhCHbonjnGyV_0Xlj7GXIHIQUL055j0tPz2FvBCizAFyIeQjtgNdy0xpqR6znYBSZQUU1RV7FsJRCKEaaJ6yK1C1UkroHfszg-LD1883nH7PKS24aeQdRbKROt6u3BP2WXQD8fsFx-giRneiND6RD8SjxzFY7-aIgbJ56teBfLpye0A3bm4bt0g7eU89Rgr8l4sHHg_EB4qHNc3OlWNwceXT_t_TDxqJJz92Sx852iUS3_alweFz9mSPfaAXl_OafX__7tvtx-zuy4dPtzd3mZVNGTPU-7YCJTstsYRW1iVqrQqtqha0LVEVBLYpbKWaZl-AQqtF2ZHtUDeESslr9vqcO_vpfqEQzeCCpb7HkaYlmKpWQqaCB4VQy6JSApJQnYXWTyF42pvZuwH9akCYjao5mgtVs1E1ACZRTb5Xl4KlHaj777pgTIK3ZwGl_zg58iZYR6OlzvlE0nSTe6DiLx_9utA</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Matsuno, Naofumi</creator><creator>Hoshino, Koyu</creator><creator>Nanri, Tomoko</creator><creator>Kawakita, Toshiro</creator><creator>Suzushima, Hitoshi</creator><creator>Kawano, Fumio</creator><creator>Mitsuya, Hiroaki</creator><creator>Asou, Norio</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>p15 mRNA expression detected by real-time quantitative reverse transcriptase-polymerase chain reaction correlates with the methylation density of the gene in adult acute leukemia</title><author>Matsuno, Naofumi ; Hoshino, Koyu ; Nanri, Tomoko ; Kawakita, Toshiro ; Suzushima, Hitoshi ; Kawano, Fumio ; Mitsuya, Hiroaki ; Asou, Norio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-a8fb6153d83a41b374a8852856b18c4a52e1c92c6599f215ac804decda89ea553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acute Disease</topic><topic>Adult</topic><topic>ALL</topic><topic>AML</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Cells - pathology</topic><topic>Burkitt Lymphoma - genetics</topic><topic>Burkitt Lymphoma - metabolism</topic><topic>Burkitt Lymphoma - pathology</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Lineage</topic><topic>CpG Islands</topic><topic>Cyclin-Dependent Kinase Inhibitor p15</topic><topic>DNA Methylation</topic><topic>Gene Expression Regulation, Leukemic</topic><topic>Humans</topic><topic>Leukemia, Myeloid - classification</topic><topic>Leukemia, Myeloid - genetics</topic><topic>Leukemia, Myeloid - metabolism</topic><topic>Methylation</topic><topic>p15</topic><topic>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics</topic><topic>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - metabolism</topic><topic>Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>RQ-PCR</topic><topic>Sequence Deletion</topic><topic>Tumor Cells, Cultured</topic><topic>Tumor Suppressor Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuno, Naofumi</creatorcontrib><creatorcontrib>Hoshino, Koyu</creatorcontrib><creatorcontrib>Nanri, Tomoko</creatorcontrib><creatorcontrib>Kawakita, Toshiro</creatorcontrib><creatorcontrib>Suzushima, Hitoshi</creatorcontrib><creatorcontrib>Kawano, Fumio</creatorcontrib><creatorcontrib>Mitsuya, Hiroaki</creatorcontrib><creatorcontrib>Asou, Norio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuno, Naofumi</au><au>Hoshino, Koyu</au><au>Nanri, Tomoko</au><au>Kawakita, Toshiro</au><au>Suzushima, Hitoshi</au><au>Kawano, Fumio</au><au>Mitsuya, Hiroaki</au><au>Asou, Norio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p15 mRNA expression detected by real-time quantitative reverse transcriptase-polymerase chain reaction correlates with the methylation density of the gene in adult acute leukemia</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>29</volume><issue>5</issue><spage>557</spage><epage>564</epage><pages>557-564</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><abstract>Cyclin-dependent kinase inhibitor
p15 is frequently inactivated by either methylation or deletion in patients with acute leukemia. To examine pathologic and clinical significance of the
p15 gene inactivation, we established a quantitative assay of
p15 mRNA expression in the bone marrow cells by real-time quantitative reverse transcriptase-polymerase chain reaction.
p15 mRNA expression in 14 patients with precursor B-cell acute lymphoblastic leukemia (PBC-ALL) well correlated with status of deletion and methylation in the
p15 gene analyzed by Southern blotting. Furthermore, two patients with PBC-ALL and 11 acute myeloblastic leukemia (AML) were quantitatively examined for
p15 gene methylation using bisulfite genomic sequencing. The data showed that
p15 mRNA expression significantly correlated with the CpG island methylation density. Among 108 AML patients,
p15 mRNA expression was significantly lower in the myeloid lineage (M1, M2, M3) than the monocytic lineage (M4, M5) (
P
=
0.0019). Above all, the majority of M3 patients showed low
p15 expression compared with M1 and M2 patients (
P
=
0.029). These observations suggest that quantitative analysis of
p15 mRNA will be useful to evaluate transcriptional repression of the
p15 gene caused by various degrees of methylation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15755508</pmid><doi>10.1016/j.leukres.2004.11.003</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-2126 |
| ispartof | Leukemia research, 2005-05, Vol.29 (5), p.557-564 |
| issn | 0145-2126 1873-5835 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67503153 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acute Disease Adult ALL AML Bone Marrow Cells - metabolism Bone Marrow Cells - pathology Burkitt Lymphoma - genetics Burkitt Lymphoma - metabolism Burkitt Lymphoma - pathology Cell Cycle Proteins - genetics Cell Lineage CpG Islands Cyclin-Dependent Kinase Inhibitor p15 DNA Methylation Gene Expression Regulation, Leukemic Humans Leukemia, Myeloid - classification Leukemia, Myeloid - genetics Leukemia, Myeloid - metabolism Methylation p15 Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - metabolism Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism RQ-PCR Sequence Deletion Tumor Cells, Cultured Tumor Suppressor Proteins - genetics |
| title | p15 mRNA expression detected by real-time quantitative reverse transcriptase-polymerase chain reaction correlates with the methylation density of the gene in adult acute leukemia |
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