Advanced glycation end products accumulate in the reproductive tract of men with diabetes

Summary Light microscopic studies comparing sperm parameters show little association between diabetes and male fertility. However, with the introduction of new analytical techniques, evidence is now emerging of previously undetectable effects of diabetes on sperm function. Specifically, a recent stu...

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Veröffentlicht in:International journal of andrology 2009-08, Vol.32 (4), p.295-305
Hauptverfasser: Mallidis, C., Agbaje, I. M., Rogers, D. A., Glenn, J. V., Pringle, R., Atkinson, A. B., Steger, K., Stitt, A. W., McClure, N.
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container_end_page 305
container_issue 4
container_start_page 295
container_title International journal of andrology
container_volume 32
creator Mallidis, C.
Agbaje, I. M.
Rogers, D. A.
Glenn, J. V.
Pringle, R.
Atkinson, A. B.
Steger, K.
Stitt, A. W.
McClure, N.
description Summary Light microscopic studies comparing sperm parameters show little association between diabetes and male fertility. However, with the introduction of new analytical techniques, evidence is now emerging of previously undetectable effects of diabetes on sperm function. Specifically, a recent study has found a significantly higher sperm nuclear DNA fragmentation in diabetic men. As advanced glycation end products (AGEs) are important instigators of oxidative stress and cell dysfunction in numerous diabetic complications, we hypothesized that these compounds could also be present in the male reproductive tract. The presence and localization of the most prominent AGE, carboxymethyl‐lysine (CML), in the human testis, epididymis and sperm was determined by immunohistochemistry. Parallel ELISA and Western blot analyses were performed to ascertain the amount of CML in seminal plasma and sperm from 13 diabetic and nine non‐diabetic subjects. CML immunoreactivity was found throughout the seminiferous epithelium, the nuclei of spermatogonia and spermatocytes, in the basal and principle cells cytoplasm and nuclei of the caput epididymis and on most sperm tails, mid pieces and all cytoplasmic droplets. The acrosomal cap, especially the equatorial band, was prominently stained in diabetic samples only. The amount of CML was significantly higher (p = 0.004) in sperm from non‐diabetic men. Considering the known detrimental actions of AGEs in other organs, the presence, location and quantity of CML, particularly the increased expression found in diabetic men, suggest that these compounds may play a hitherto unrecognized role in male infertility.
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The presence and localization of the most prominent AGE, carboxymethyl‐lysine (CML), in the human testis, epididymis and sperm was determined by immunohistochemistry. Parallel ELISA and Western blot analyses were performed to ascertain the amount of CML in seminal plasma and sperm from 13 diabetic and nine non‐diabetic subjects. CML immunoreactivity was found throughout the seminiferous epithelium, the nuclei of spermatogonia and spermatocytes, in the basal and principle cells cytoplasm and nuclei of the caput epididymis and on most sperm tails, mid pieces and all cytoplasmic droplets. The acrosomal cap, especially the equatorial band, was prominently stained in diabetic samples only. The amount of CML was significantly higher (p = 0.004) in sperm from non‐diabetic men. 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W.</au><au>McClure, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Advanced glycation end products accumulate in the reproductive tract of men with diabetes</atitle><jtitle>International journal of andrology</jtitle><addtitle>Int J Androl</addtitle><date>2009-08</date><risdate>2009</risdate><volume>32</volume><issue>4</issue><spage>295</spage><epage>305</epage><pages>295-305</pages><issn>0105-6263</issn><eissn>1365-2605</eissn><coden>IJANDP</coden><abstract>Summary Light microscopic studies comparing sperm parameters show little association between diabetes and male fertility. However, with the introduction of new analytical techniques, evidence is now emerging of previously undetectable effects of diabetes on sperm function. Specifically, a recent study has found a significantly higher sperm nuclear DNA fragmentation in diabetic men. 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The amount of CML was significantly higher (p = 0.004) in sperm from non‐diabetic men. Considering the known detrimental actions of AGEs in other organs, the presence, location and quantity of CML, particularly the increased expression found in diabetic men, suggest that these compounds may play a hitherto unrecognized role in male infertility.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18217985</pmid><doi>10.1111/j.1365-2605.2007.00849.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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subjects Adult
AGEs
Biological and medical sciences
Blotting, Western
Case-Control Studies
diabetes
Diabetes Complications - etiology
Diabetes Complications - metabolism
Diabetes Mellitus - metabolism
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Enzyme-Linked Immunosorbent Assay
epididymis
Epididymis - chemistry
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Fundamental and applied biological sciences. Psychology
Glycation End Products, Advanced - analysis
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Infertility, Male - etiology
Infertility, Male - metabolism
Lysine - analogs & derivatives
Lysine - analysis
Male
Male genital diseases
Mammalian male genital system
Medical sciences
Semen - chemistry
spermatozoa
Spermatozoa - chemistry
testis
Testis - chemistry
Vertebrates: reproduction
title Advanced glycation end products accumulate in the reproductive tract of men with diabetes
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