Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites
The effect of 8-isoprostanes on potassium (K+)-depolarization-evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K(+)-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF(2alp...
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| Veröffentlicht in: | Neurochemical research 2005-01, Vol.30 (1), p.129-137 |
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| creator | Opere, Catherine A Zheng, Wei Dong Huang, Jifan Adewale, Adeniran Kruglet, Michael Ohia, Sunny E |
| description | The effect of 8-isoprostanes on potassium (K+)-depolarization-evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K(+)-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF(2alpha) (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 microM) enhanced K(+)-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF(2alpha) was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 microM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF(2alpha) (1-30 microM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 microM). In conclusion, 8-isoPGF(2alpha) exhibits a dual regulatory effect on K(+)-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF(2alpha) is due to the activation of EP1/EP2 receptors while the excitatory effects are due to the activation of thromboxane receptors. |
| doi_str_mv | 10.1007/s11064-004-9694-3 |
| format | Article |
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Isolated bovine retinae were prepared for studies of K(+)-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF(2alpha) (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 microM) enhanced K(+)-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF(2alpha) was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 microM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF(2alpha) (1-30 microM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 microM). In conclusion, 8-isoPGF(2alpha) exhibits a dual regulatory effect on K(+)-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF(2alpha) is due to the activation of EP1/EP2 receptors while the excitatory effects are due to the activation of thromboxane receptors.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-004-9694-3</identifier><identifier>PMID: 15756941</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Arachidonic Acid - metabolism ; Aspartic Acid - metabolism ; Cattle ; Dose-Response Relationship, Drug ; In Vitro Techniques ; Isoprostanes - pharmacology ; Retina - drug effects ; Retina - metabolism ; Tritium</subject><ispartof>Neurochemical research, 2005-01, Vol.30 (1), p.129-137</ispartof><rights>Springer Science+Business Media, Inc. 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-e4778bce1f2be911fa0ca7e85e4a231698cee86531a6acb6e89849030fe918c63</citedby><cites>FETCH-LOGICAL-c357t-e4778bce1f2be911fa0ca7e85e4a231698cee86531a6acb6e89849030fe918c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15756941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Opere, Catherine A</creatorcontrib><creatorcontrib>Zheng, Wei Dong</creatorcontrib><creatorcontrib>Huang, Jifan</creatorcontrib><creatorcontrib>Adewale, Adeniran</creatorcontrib><creatorcontrib>Kruglet, Michael</creatorcontrib><creatorcontrib>Ohia, Sunny E</creatorcontrib><title>Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>The effect of 8-isoprostanes on potassium (K+)-depolarization-evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K(+)-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF(2alpha) (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 microM) enhanced K(+)-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF(2alpha) was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 microM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF(2alpha) (1-30 microM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 microM). In conclusion, 8-isoPGF(2alpha) exhibits a dual regulatory effect on K(+)-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF(2alpha) is due to the activation of EP1/EP2 receptors while the excitatory effects are due to the activation of thromboxane receptors.</description><subject>Animals</subject><subject>Arachidonic Acid - metabolism</subject><subject>Aspartic Acid - metabolism</subject><subject>Cattle</subject><subject>Dose-Response Relationship, Drug</subject><subject>In Vitro Techniques</subject><subject>Isoprostanes - pharmacology</subject><subject>Retina - drug effects</subject><subject>Retina - metabolism</subject><subject>Tritium</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1rFTEUhoMo9lr9AW4kuHA3mjPJ5MOdtNoKBTe6EgmZ3BOaMjO5JhnBnT_dTO8FoRtXIZznOfCel5CXwN4CY-pdAWBSdIyJzkgjOv6I7GBQvJOG8cdkx3ibcjDsjDwr5Y6xZvXwlJw1aGgC7Mify9VNFENAX2kKNJZ0yKlUt2ChaaH1FmnGCV3BbfydX_-47Fw5uFxdRRpymjdnap89HdOvuGx8jYvD9zSn6d5y2fnbuE9L9NT5uKczVjemKVYsz8mT4KaCL07vOfn26ePXi-vu5svV54sPN53ng6odCqX06BFCP6IBCI55p1APKFzPQRrtEbUcODjp_ChRGy3aFVhotPaSn5M3x70t3s8VS7VzLB6nqSVNa7FSCaO5gP-CoLjo5T34-gF4l9a8tBC270GD6Y1qEBwh365aMgZ7yHF2-bcFZrcS7bFE20q0W4mWN-fVafE6zrj_Z5xa438BJkSYOA</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Opere, Catherine A</creator><creator>Zheng, Wei Dong</creator><creator>Huang, Jifan</creator><creator>Adewale, Adeniran</creator><creator>Kruglet, Michael</creator><creator>Ohia, Sunny E</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites</title><author>Opere, Catherine A ; 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Isolated bovine retinae were prepared for studies of K(+)-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF(2alpha) (1-100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM-30 microM) enhanced K(+)-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF(2alpha) was mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 microM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF(2alpha) (1-30 microM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 microM). In conclusion, 8-isoPGF(2alpha) exhibits a dual regulatory effect on K(+)-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF(2alpha) is due to the activation of EP1/EP2 receptors while the excitatory effects are due to the activation of thromboxane receptors.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>15756941</pmid><doi>10.1007/s11064-004-9694-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | Neurochemical research, 2005-01, Vol.30 (1), p.129-137 |
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| subjects | Animals Arachidonic Acid - metabolism Aspartic Acid - metabolism Cattle Dose-Response Relationship, Drug In Vitro Techniques Isoprostanes - pharmacology Retina - drug effects Retina - metabolism Tritium |
| title | Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites |
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