MHC class I and II expression in juvenile dermatomyositis skeletal muscle
To assess MHC I and II expressions in muscle fibres of juvenile dermatomyositis (JDM) and compare with the expression in polymyositis (PM), dermatomyositis (DM) and dystrophy. Forty-eight JDM patients and 17 controls (8 PM, 5 DM and 4 dystrophy) were studied. The mean age at disease onset was 7.1+/-...
Gespeichert in:
| Veröffentlicht in: | Clinical and experimental rheumatology 2009-05, Vol.27 (3), p.519-526 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 526 |
|---|---|
| container_issue | 3 |
| container_start_page | 519 |
| container_title | Clinical and experimental rheumatology |
| container_volume | 27 |
| creator | SALLUM, A. M. E KISS, M. H. B SILVA, C. A. A WAKAMATSU, A SACHETTI, S LOTUFO, S MATSUMURA, N MARIE, S. K. N |
| description | To assess MHC I and II expressions in muscle fibres of juvenile dermatomyositis (JDM) and compare with the expression in polymyositis (PM), dermatomyositis (DM) and dystrophy.
Forty-eight JDM patients and 17 controls (8 PM, 5 DM and 4 dystrophy) were studied. The mean age at disease onset was 7.1+/-3.0 years and the mean duration of weakness before biopsy was 9.4+/-12.9 months. Routinehistochemistry and immunohistochemistry (StreptABComplex/HRP) for MHC I and II (Dakopatts) were performed on serial frozen muscle sections in all patients. Mann-Whitney, Kruskal Wallis, chi-square and Fisher's exact statistical methods were used.
MHC I expression was positive in 47 (97.9%) JDM cases. This expression was observed independent of time of disease, corticotherapy previous to muscle biopsy and to the grading of inflammation observed in clinical, laboratorial and histological parameters. The expression of MHC I was similar on JDM, PM and DM, and lower in dystrophy. On the other hand, MHC II expression was positive in just 28.2% of JDM cases and was correlated to histological features as inflammatory infiltrate, increased connective tissue and VAS for global degree of abnormality (p |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_67495696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67495696</sourcerecordid><originalsourceid>FETCH-LOGICAL-p239t-b432c4853c648afadfa1aa375e678adf205050163f53fdbdf7f9b07bd6766f863</originalsourceid><addsrcrecordid>eNo9z0tLxDAUBeAgijOO_gXJRneFNM9mKYM6hRE3CrMrt2kCGdOHva3ov3fEUe7icODjwD0hy1xZkTFb7E7JkgnLs0Lp3YJcIO4Z41ppc04WudVMSmmXpHzarKlLgEhLCl1Dy5L6z2H0iLHvaOzofv7wXUyeNn5sYerbrx7jFJHim09-gkTbGV3yl-QsQEJ_dcwVeX24f1lvsu3zY7m-22YDF3bKaim4k4USTssCAjQBcgBhlNemODTO1OFyLYISoambYIKtmakbbbQOhRYrcvu7O4z9--xxqtqIzqcEne9nrLSRVmn7A6-PcK5b31TDGFsYv6q_5w_g5ggAHaQwQuci_jueG8m5MOIb-3Nj_Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67495696</pqid></control><display><type>article</type><title>MHC class I and II expression in juvenile dermatomyositis skeletal muscle</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>SALLUM, A. M. E ; KISS, M. H. B ; SILVA, C. A. A ; WAKAMATSU, A ; SACHETTI, S ; LOTUFO, S ; MATSUMURA, N ; MARIE, S. K. N</creator><creatorcontrib>SALLUM, A. M. E ; KISS, M. H. B ; SILVA, C. A. A ; WAKAMATSU, A ; SACHETTI, S ; LOTUFO, S ; MATSUMURA, N ; MARIE, S. K. N</creatorcontrib><description>To assess MHC I and II expressions in muscle fibres of juvenile dermatomyositis (JDM) and compare with the expression in polymyositis (PM), dermatomyositis (DM) and dystrophy.
Forty-eight JDM patients and 17 controls (8 PM, 5 DM and 4 dystrophy) were studied. The mean age at disease onset was 7.1+/-3.0 years and the mean duration of weakness before biopsy was 9.4+/-12.9 months. Routinehistochemistry and immunohistochemistry (StreptABComplex/HRP) for MHC I and II (Dakopatts) were performed on serial frozen muscle sections in all patients. Mann-Whitney, Kruskal Wallis, chi-square and Fisher's exact statistical methods were used.
MHC I expression was positive in 47 (97.9%) JDM cases. This expression was observed independent of time of disease, corticotherapy previous to muscle biopsy and to the grading of inflammation observed in clinical, laboratorial and histological parameters. The expression of MHC I was similar on JDM, PM and DM, and lower in dystrophy. On the other hand, MHC II expression was positive in just 28.2% of JDM cases and was correlated to histological features as inflammatory infiltrate, increased connective tissue and VAS for global degree of abnormality (p<0.05). MHC II expression was similar in DM/PM and lower in JDM and dystrophy, and it was based on the frequency of positive staining rather than to the degree of the MCH II expression.
MHC I expression in muscle fibres is a premature and late marker of JDM patient independent to corticotherapy, and MHC II expression was lower in JDM than in PM and DM.</description><identifier>ISSN: 0392-856X</identifier><identifier>EISSN: 1593-098X</identifier><identifier>PMID: 19604449</identifier><language>eng</language><publisher>Pisa: Clinical and Experimental Rheumatology</publisher><subject>Biological and medical sciences ; Biomarkers - metabolism ; Biopsy ; Child ; Child, Preschool ; Cross-Sectional Studies ; Dermatomyositis - diagnosis ; Dermatomyositis - metabolism ; Dermatomyositis - pathology ; Diagnosis, Differential ; Diseases of striated muscles. Neuromuscular diseases ; Diseases of the osteoarticular system ; Gene Expression Regulation ; Genes, MHC Class I - genetics ; Genes, MHC Class II - genetics ; HLA Antigens - metabolism ; Humans ; Medical sciences ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - pathology ; Muscular Dystrophies - diagnosis ; Muscular Dystrophies - metabolism ; Muscular Dystrophies - pathology ; Neurology ; Polymyositis - diagnosis ; Polymyositis - metabolism ; Polymyositis - pathology ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><ispartof>Clinical and experimental rheumatology, 2009-05, Vol.27 (3), p.519-526</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21742237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19604449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SALLUM, A. M. E</creatorcontrib><creatorcontrib>KISS, M. H. B</creatorcontrib><creatorcontrib>SILVA, C. A. A</creatorcontrib><creatorcontrib>WAKAMATSU, A</creatorcontrib><creatorcontrib>SACHETTI, S</creatorcontrib><creatorcontrib>LOTUFO, S</creatorcontrib><creatorcontrib>MATSUMURA, N</creatorcontrib><creatorcontrib>MARIE, S. K. N</creatorcontrib><title>MHC class I and II expression in juvenile dermatomyositis skeletal muscle</title><title>Clinical and experimental rheumatology</title><addtitle>Clin Exp Rheumatol</addtitle><description>To assess MHC I and II expressions in muscle fibres of juvenile dermatomyositis (JDM) and compare with the expression in polymyositis (PM), dermatomyositis (DM) and dystrophy.
Forty-eight JDM patients and 17 controls (8 PM, 5 DM and 4 dystrophy) were studied. The mean age at disease onset was 7.1+/-3.0 years and the mean duration of weakness before biopsy was 9.4+/-12.9 months. Routinehistochemistry and immunohistochemistry (StreptABComplex/HRP) for MHC I and II (Dakopatts) were performed on serial frozen muscle sections in all patients. Mann-Whitney, Kruskal Wallis, chi-square and Fisher's exact statistical methods were used.
MHC I expression was positive in 47 (97.9%) JDM cases. This expression was observed independent of time of disease, corticotherapy previous to muscle biopsy and to the grading of inflammation observed in clinical, laboratorial and histological parameters. The expression of MHC I was similar on JDM, PM and DM, and lower in dystrophy. On the other hand, MHC II expression was positive in just 28.2% of JDM cases and was correlated to histological features as inflammatory infiltrate, increased connective tissue and VAS for global degree of abnormality (p<0.05). MHC II expression was similar in DM/PM and lower in JDM and dystrophy, and it was based on the frequency of positive staining rather than to the degree of the MCH II expression.
MHC I expression in muscle fibres is a premature and late marker of JDM patient independent to corticotherapy, and MHC II expression was lower in JDM than in PM and DM.</description><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biopsy</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross-Sectional Studies</subject><subject>Dermatomyositis - diagnosis</subject><subject>Dermatomyositis - metabolism</subject><subject>Dermatomyositis - pathology</subject><subject>Diagnosis, Differential</subject><subject>Diseases of striated muscles. Neuromuscular diseases</subject><subject>Diseases of the osteoarticular system</subject><subject>Gene Expression Regulation</subject><subject>Genes, MHC Class I - genetics</subject><subject>Genes, MHC Class II - genetics</subject><subject>HLA Antigens - metabolism</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscular Dystrophies - diagnosis</subject><subject>Muscular Dystrophies - metabolism</subject><subject>Muscular Dystrophies - pathology</subject><subject>Neurology</subject><subject>Polymyositis - diagnosis</subject><subject>Polymyositis - metabolism</subject><subject>Polymyositis - pathology</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><issn>0392-856X</issn><issn>1593-098X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9z0tLxDAUBeAgijOO_gXJRneFNM9mKYM6hRE3CrMrt2kCGdOHva3ov3fEUe7icODjwD0hy1xZkTFb7E7JkgnLs0Lp3YJcIO4Z41ppc04WudVMSmmXpHzarKlLgEhLCl1Dy5L6z2H0iLHvaOzofv7wXUyeNn5sYerbrx7jFJHim09-gkTbGV3yl-QsQEJ_dcwVeX24f1lvsu3zY7m-22YDF3bKaim4k4USTssCAjQBcgBhlNemODTO1OFyLYISoambYIKtmakbbbQOhRYrcvu7O4z9--xxqtqIzqcEne9nrLSRVmn7A6-PcK5b31TDGFsYv6q_5w_g5ggAHaQwQuci_jueG8m5MOIb-3Nj_Q</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>SALLUM, A. M. E</creator><creator>KISS, M. H. B</creator><creator>SILVA, C. A. A</creator><creator>WAKAMATSU, A</creator><creator>SACHETTI, S</creator><creator>LOTUFO, S</creator><creator>MATSUMURA, N</creator><creator>MARIE, S. K. N</creator><general>Clinical and Experimental Rheumatology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>MHC class I and II expression in juvenile dermatomyositis skeletal muscle</title><author>SALLUM, A. M. E ; KISS, M. H. B ; SILVA, C. A. A ; WAKAMATSU, A ; SACHETTI, S ; LOTUFO, S ; MATSUMURA, N ; MARIE, S. K. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-b432c4853c648afadfa1aa375e678adf205050163f53fdbdf7f9b07bd6766f863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biopsy</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross-Sectional Studies</topic><topic>Dermatomyositis - diagnosis</topic><topic>Dermatomyositis - metabolism</topic><topic>Dermatomyositis - pathology</topic><topic>Diagnosis, Differential</topic><topic>Diseases of striated muscles. Neuromuscular diseases</topic><topic>Diseases of the osteoarticular system</topic><topic>Gene Expression Regulation</topic><topic>Genes, MHC Class I - genetics</topic><topic>Genes, MHC Class II - genetics</topic><topic>HLA Antigens - metabolism</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - pathology</topic><topic>Muscular Dystrophies - diagnosis</topic><topic>Muscular Dystrophies - metabolism</topic><topic>Muscular Dystrophies - pathology</topic><topic>Neurology</topic><topic>Polymyositis - diagnosis</topic><topic>Polymyositis - metabolism</topic><topic>Polymyositis - pathology</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SALLUM, A. M. E</creatorcontrib><creatorcontrib>KISS, M. H. B</creatorcontrib><creatorcontrib>SILVA, C. A. A</creatorcontrib><creatorcontrib>WAKAMATSU, A</creatorcontrib><creatorcontrib>SACHETTI, S</creatorcontrib><creatorcontrib>LOTUFO, S</creatorcontrib><creatorcontrib>MATSUMURA, N</creatorcontrib><creatorcontrib>MARIE, S. K. N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SALLUM, A. M. E</au><au>KISS, M. H. B</au><au>SILVA, C. A. A</au><au>WAKAMATSU, A</au><au>SACHETTI, S</au><au>LOTUFO, S</au><au>MATSUMURA, N</au><au>MARIE, S. K. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MHC class I and II expression in juvenile dermatomyositis skeletal muscle</atitle><jtitle>Clinical and experimental rheumatology</jtitle><addtitle>Clin Exp Rheumatol</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>27</volume><issue>3</issue><spage>519</spage><epage>526</epage><pages>519-526</pages><issn>0392-856X</issn><eissn>1593-098X</eissn><abstract>To assess MHC I and II expressions in muscle fibres of juvenile dermatomyositis (JDM) and compare with the expression in polymyositis (PM), dermatomyositis (DM) and dystrophy.
Forty-eight JDM patients and 17 controls (8 PM, 5 DM and 4 dystrophy) were studied. The mean age at disease onset was 7.1+/-3.0 years and the mean duration of weakness before biopsy was 9.4+/-12.9 months. Routinehistochemistry and immunohistochemistry (StreptABComplex/HRP) for MHC I and II (Dakopatts) were performed on serial frozen muscle sections in all patients. Mann-Whitney, Kruskal Wallis, chi-square and Fisher's exact statistical methods were used.
MHC I expression was positive in 47 (97.9%) JDM cases. This expression was observed independent of time of disease, corticotherapy previous to muscle biopsy and to the grading of inflammation observed in clinical, laboratorial and histological parameters. The expression of MHC I was similar on JDM, PM and DM, and lower in dystrophy. On the other hand, MHC II expression was positive in just 28.2% of JDM cases and was correlated to histological features as inflammatory infiltrate, increased connective tissue and VAS for global degree of abnormality (p<0.05). MHC II expression was similar in DM/PM and lower in JDM and dystrophy, and it was based on the frequency of positive staining rather than to the degree of the MCH II expression.
MHC I expression in muscle fibres is a premature and late marker of JDM patient independent to corticotherapy, and MHC II expression was lower in JDM than in PM and DM.</abstract><cop>Pisa</cop><pub>Clinical and Experimental Rheumatology</pub><pmid>19604449</pmid><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0392-856X |
| ispartof | Clinical and experimental rheumatology, 2009-05, Vol.27 (3), p.519-526 |
| issn | 0392-856X 1593-098X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67495696 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biomarkers - metabolism Biopsy Child Child, Preschool Cross-Sectional Studies Dermatomyositis - diagnosis Dermatomyositis - metabolism Dermatomyositis - pathology Diagnosis, Differential Diseases of striated muscles. Neuromuscular diseases Diseases of the osteoarticular system Gene Expression Regulation Genes, MHC Class I - genetics Genes, MHC Class II - genetics HLA Antigens - metabolism Humans Medical sciences Muscle, Skeletal - metabolism Muscle, Skeletal - pathology Muscular Dystrophies - diagnosis Muscular Dystrophies - metabolism Muscular Dystrophies - pathology Neurology Polymyositis - diagnosis Polymyositis - metabolism Polymyositis - pathology Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis |
| title | MHC class I and II expression in juvenile dermatomyositis skeletal muscle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T05%3A50%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MHC%20class%20I%20and%20II%20expression%20in%20juvenile%20dermatomyositis%20skeletal%20muscle&rft.jtitle=Clinical%20and%20experimental%20rheumatology&rft.au=SALLUM,%20A.%20M.%20E&rft.date=2009-05-01&rft.volume=27&rft.issue=3&rft.spage=519&rft.epage=526&rft.pages=519-526&rft.issn=0392-856X&rft.eissn=1593-098X&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E67495696%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67495696&rft_id=info:pmid/19604449&rfr_iscdi=true |