The Klebsiella pneumoniae nitrogenase Fe protein gene ( nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii

The entire coding region of chlL, an essential chloroplast gene required for chlorophyll biosynthesis in the dark in Chlamydomonas reinhardtii, was precisely replaced by either the Klebsiella pneumoniae nifH (encoding the structural component of nitrogenase Fe protein) or the Escherichia coli uidA r...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-04, Vol.329 (3), p.966-975
Hauptverfasser:	Cheng, Qi, Day, Anil, Dowson-Day, Mandy, Shen, Gui-Fang, Dixon, Ray
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cells, Cultured Chlamydomonas reinhardtii Chlamydomonas reinhardtii - cytology Chlamydomonas reinhardtii - enzymology Chlamydomonas reinhardtii - genetics Chlorophyll - biosynthesis Chlorophyll biosynthesis pathway Chloroplast transformation Chloroplasts - genetics Chloroplasts - metabolism Cloning, Molecular - methods Escherichia coli Fe protein Heteroplasmic Homologous recombination Homoplasmic Klebsiella pneumoniae Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics Light-dependent protochlorophyllide reductase Light-independent protochlorophyllide reductase Oxidoreductases - genetics Oxidoreductases - metabolism Protein Engineering - methods Recombinant Proteins - metabolism Transplastomic
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