Brightness of Yellow Fluorescent Protein from Coral (zFP538) Depends on Aggregation

The yellow fluorescent protein from coral (zFP538) forms aggregates in water solutions. According to dynamic light scattering and gel filtration data, the aggregation number is ∼1000−10000 at pH 8−9 and protein concentration 1 mg/mL. Gel filtration demonstrated that dissociation of the aggregates ta...

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Veröffentlicht in:	Biochemistry (Easton) 2005-03, Vol.44 (10), p.3982-3993
Hauptverfasser:	Zubova, Nadezhda N, Korolenko, Vadim A, Astafyev, Artem A, Petrukhin, Andrew N, Vinokurov, Leonid M, Sarkisov, Oleg M, Savitsky, Alexander P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anthozoa - chemistry Hydrogen-Ion Concentration Kinetics Luminescent Proteins - chemistry Luminescent Proteins - metabolism Microscopy, Atomic Force - methods Models, Chemical Molecular Weight Nonlinear Dynamics Protein Denaturation Solutions Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Spectrum Analysis - methods Titrimetry
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