Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes

Enhancer trap is a powerful approach for identifying tissue- and stage-specific gene expression in plants and animals. For Arabidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully used to identify tissue-specific gene expression in many plant organs. Howe...

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Veröffentlicht in:The Plant journal : for cell and molecular biology 2005-03, Vol.41 (6), p.936-944
Hauptverfasser: Liu, P.P, Koizuka, N, Homrichhausen, T.M, Hewitt, J.R, Martin, R.C, Nonogaki, H
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container_issue 6
container_start_page 936
container_title The Plant journal : for cell and molecular biology
container_volume 41
creator Liu, P.P
Koizuka, N
Homrichhausen, T.M
Hewitt, J.R
Martin, R.C
Nonogaki, H
description Enhancer trap is a powerful approach for identifying tissue- and stage-specific gene expression in plants and animals. For Arabidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully used to identify tissue-specific gene expression in many plant organs. However, limited applications of these lines for seed germination research have been reported. This is probably due to the impermeability of the testa to the GUS substrate. By focusing on the stages between testa and endosperm rupture; we were able to circumvent the testa barrier to the GUS substrate and observe diverse tissue-specific gene expression during germination sensu stricto. One hundred and twenty-one positive subpools of 10 lines out of 1130 were isolated. Approximately 4500 plants from these subpools were grown in a greenhouse and one to seven individual plants exhibiting GUS expression in seeds were isolated for each subpool. This library of the Arabidopsis seed enhancer-trap lines is an efficient tool for identifying seed germination-associated genes. The individual lines from this library will be provided to the international seed biology research community. International collaboration to identify the trapped genes using genome-walking PCR and to characterize the gene functions using knockout plants will significantly enhance our understanding of the molecular mechanisms of seed germination.
doi_str_mv 10.1111/j.1365-313X.2005.02347.x
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For Arabidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully used to identify tissue-specific gene expression in many plant organs. However, limited applications of these lines for seed germination research have been reported. This is probably due to the impermeability of the testa to the GUS substrate. By focusing on the stages between testa and endosperm rupture; we were able to circumvent the testa barrier to the GUS substrate and observe diverse tissue-specific gene expression during germination sensu stricto. One hundred and twenty-one positive subpools of 10 lines out of 1130 were isolated. Approximately 4500 plants from these subpools were grown in a greenhouse and one to seven individual plants exhibiting GUS expression in seeds were isolated for each subpool. This library of the Arabidopsis seed enhancer-trap lines is an efficient tool for identifying seed germination-associated genes. 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Psychology ; Gene expression ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Gene Library ; Genes ; Germination ; Germination - genetics ; Germination and dormancy ; Glucuronidase - metabolism ; molecular sequence data ; nucleotide sequences ; Plant physiology and development ; reporter genes ; screening ; seed germination ; Seeds ; Seeds - metabolism ; Seeds - ultrastructure ; transgenes</subject><ispartof>The Plant journal : for cell and molecular biology, 2005-03, Vol.41 (6), p.936-944</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Mar 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5457-b843637919369168fb340bd056cf45c3f029fe9f6ac183caeaaa1cbc4af8a4513</citedby><cites>FETCH-LOGICAL-c5457-b843637919369168fb340bd056cf45c3f029fe9f6ac183caeaaa1cbc4af8a4513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2005.02347.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2005.02347.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=16568153$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15743455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, P.P</creatorcontrib><creatorcontrib>Koizuka, N</creatorcontrib><creatorcontrib>Homrichhausen, T.M</creatorcontrib><creatorcontrib>Hewitt, J.R</creatorcontrib><creatorcontrib>Martin, R.C</creatorcontrib><creatorcontrib>Nonogaki, H</creatorcontrib><title>Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Enhancer trap is a powerful approach for identifying tissue- and stage-specific gene expression in plants and animals. For Arabidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully used to identify tissue-specific gene expression in many plant organs. However, limited applications of these lines for seed germination research have been reported. This is probably due to the impermeability of the testa to the GUS substrate. By focusing on the stages between testa and endosperm rupture; we were able to circumvent the testa barrier to the GUS substrate and observe diverse tissue-specific gene expression during germination sensu stricto. One hundred and twenty-one positive subpools of 10 lines out of 1130 were isolated. Approximately 4500 plants from these subpools were grown in a greenhouse and one to seven individual plants exhibiting GUS expression in seeds were isolated for each subpool. This library of the Arabidopsis seed enhancer-trap lines is an efficient tool for identifying seed germination-associated genes. 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Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Germination</topic><topic>Germination - genetics</topic><topic>Germination and dormancy</topic><topic>Glucuronidase - metabolism</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Plant physiology and development</topic><topic>reporter genes</topic><topic>screening</topic><topic>seed germination</topic><topic>Seeds</topic><topic>Seeds - metabolism</topic><topic>Seeds - ultrastructure</topic><topic>transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, P.P</creatorcontrib><creatorcontrib>Koizuka, N</creatorcontrib><creatorcontrib>Homrichhausen, T.M</creatorcontrib><creatorcontrib>Hewitt, J.R</creatorcontrib><creatorcontrib>Martin, R.C</creatorcontrib><creatorcontrib>Nonogaki, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, P.P</au><au>Koizuka, N</au><au>Homrichhausen, T.M</au><au>Hewitt, J.R</au><au>Martin, R.C</au><au>Nonogaki, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2005-03</date><risdate>2005</risdate><volume>41</volume><issue>6</issue><spage>936</spage><epage>944</epage><pages>936-944</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Enhancer trap is a powerful approach for identifying tissue- and stage-specific gene expression in plants and animals. For Arabidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully used to identify tissue-specific gene expression in many plant organs. However, limited applications of these lines for seed germination research have been reported. This is probably due to the impermeability of the testa to the GUS substrate. By focusing on the stages between testa and endosperm rupture; we were able to circumvent the testa barrier to the GUS substrate and observe diverse tissue-specific gene expression during germination sensu stricto. One hundred and twenty-one positive subpools of 10 lines out of 1130 were isolated. Approximately 4500 plants from these subpools were grown in a greenhouse and one to seven individual plants exhibiting GUS expression in seeds were isolated for each subpool. This library of the Arabidopsis seed enhancer-trap lines is an efficient tool for identifying seed germination-associated genes. The individual lines from this library will be provided to the international seed biology research community. International collaboration to identify the trapped genes using genome-walking PCR and to characterize the gene functions using knockout plants will significantly enhance our understanding of the molecular mechanisms of seed germination.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15743455</pmid><doi>10.1111/j.1365-313X.2005.02347.x</doi><tpages>9</tpages></addata></record>
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subjects Arabidopsis
Arabidopsis - embryology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
beta-glucuronidase
Biological and medical sciences
Botany
embryo
endosperm
enhancer elements
enhancer trap
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Library
Genes
Germination
Germination - genetics
Germination and dormancy
Glucuronidase - metabolism
molecular sequence data
nucleotide sequences
Plant physiology and development
reporter genes
screening
seed germination
Seeds
Seeds - metabolism
Seeds - ultrastructure
transgenes
title Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes
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