The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice
The quality control of sperm is critical for efficient reproduction. In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is...
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| Veröffentlicht in: | Development, growth & differentiation growth & differentiation, 2005-01, Vol.47 (1), p.15-24 |
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| creator | Kido, Tatsuo Arata, Satoru Suzuki, Ryusuke Hosono, Tomohiko Nakanishi, Yoshinobu Miyazaki, Jun‐ichi Saito, Izumu Kuroki, Toshio Shioda, Seiji |
| description | The quality control of sperm is critical for efficient reproduction. In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is related to fertility. Composition of the fatty acid of germ cells changes dynamically during spermatogenesis, and fatty acid binding protein (FABP) may be involved in these changes. In this study, we developed transgenic mice with a testicular germ‐cell‐specific FABP (PERF15) transgene, whose expression was controlled by the Cre‐LoxP site‐specific recombination system. We also developed transgenic mice with the Cre gene under the control of the spermatocyte specific Pgk2 promoter. In double transgenic mice, following Cre‐mediated recombination of the PERF15 containing transgene, PERF15 was strongly overexpressed. Its overexpression induced multinucleate symplasts to form, indicating programmed germ cell death occurred at the elongated spermatid stage. As a result, sperm harboring the transgene were significantly decreased, but the surviving sperm demonstrated higher fertility than natural sperm. Therefore, we conclude that PERF15 associate with the direction of germ cell fates and preserve the quality of sperm. |
| doi_str_mv | 10.1111/j.1440-169x.2004.00775.x |
| format | Article |
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In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is related to fertility. Composition of the fatty acid of germ cells changes dynamically during spermatogenesis, and fatty acid binding protein (FABP) may be involved in these changes. In this study, we developed transgenic mice with a testicular germ‐cell‐specific FABP (PERF15) transgene, whose expression was controlled by the Cre‐LoxP site‐specific recombination system. We also developed transgenic mice with the Cre gene under the control of the spermatocyte specific Pgk2 promoter. In double transgenic mice, following Cre‐mediated recombination of the PERF15 containing transgene, PERF15 was strongly overexpressed. Its overexpression induced multinucleate symplasts to form, indicating programmed germ cell death occurred at the elongated spermatid stage. As a result, sperm harboring the transgene were significantly decreased, but the surviving sperm demonstrated higher fertility than natural sperm. Therefore, we conclude that PERF15 associate with the direction of germ cell fates and preserve the quality of sperm.</description><identifier>ISSN: 0012-1592</identifier><identifier>EISSN: 1440-169X</identifier><identifier>DOI: 10.1111/j.1440-169x.2004.00775.x</identifier><identifier>PMID: 15740583</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Pty</publisher><subject>Animals ; Apoptosis - physiology ; Carrier Proteins - metabolism ; Cre‐LoxP system ; DNA Primers ; fatty acid binding protein ; Fatty Acid-Binding Proteins ; Fertility - genetics ; germ cell death ; Green Fluorescent Proteins ; Immunoblotting ; Immunohistochemistry ; Integrases - genetics ; Isoenzymes - genetics ; Male ; Mice ; Mice, Transgenic ; PERF15 ; Phosphoglycerate Kinase - genetics ; Promoter Regions, Genetic - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Spermatogenesis - physiology ; Spermatozoa - metabolism ; Spermatozoa - physiology ; testis ; Transgenes - genetics</subject><ispartof>Development, growth & differentiation, 2005-01, Vol.47 (1), p.15-24</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4775-ccfae433611605d513b59aabe772882e8d0d6d9f1527640d5c93a40eb82434933</citedby><cites>FETCH-LOGICAL-c4775-ccfae433611605d513b59aabe772882e8d0d6d9f1527640d5c93a40eb82434933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-169x.2004.00775.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-169x.2004.00775.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27903,27904,45553,45554,46388,46812</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15740583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kido, Tatsuo</creatorcontrib><creatorcontrib>Arata, Satoru</creatorcontrib><creatorcontrib>Suzuki, Ryusuke</creatorcontrib><creatorcontrib>Hosono, Tomohiko</creatorcontrib><creatorcontrib>Nakanishi, Yoshinobu</creatorcontrib><creatorcontrib>Miyazaki, Jun‐ichi</creatorcontrib><creatorcontrib>Saito, Izumu</creatorcontrib><creatorcontrib>Kuroki, Toshio</creatorcontrib><creatorcontrib>Shioda, Seiji</creatorcontrib><title>The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice</title><title>Development, growth & differentiation</title><addtitle>Dev Growth Differ</addtitle><description>The quality control of sperm is critical for efficient reproduction. In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is related to fertility. Composition of the fatty acid of germ cells changes dynamically during spermatogenesis, and fatty acid binding protein (FABP) may be involved in these changes. In this study, we developed transgenic mice with a testicular germ‐cell‐specific FABP (PERF15) transgene, whose expression was controlled by the Cre‐LoxP site‐specific recombination system. We also developed transgenic mice with the Cre gene under the control of the spermatocyte specific Pgk2 promoter. In double transgenic mice, following Cre‐mediated recombination of the PERF15 containing transgene, PERF15 was strongly overexpressed. Its overexpression induced multinucleate symplasts to form, indicating programmed germ cell death occurred at the elongated spermatid stage. As a result, sperm harboring the transgene were significantly decreased, but the surviving sperm demonstrated higher fertility than natural sperm. Therefore, we conclude that PERF15 associate with the direction of germ cell fates and preserve the quality of sperm.</description><subject>Animals</subject><subject>Apoptosis - physiology</subject><subject>Carrier Proteins - metabolism</subject><subject>Cre‐LoxP system</subject><subject>DNA Primers</subject><subject>fatty acid binding protein</subject><subject>Fatty Acid-Binding Proteins</subject><subject>Fertility - genetics</subject><subject>germ cell death</subject><subject>Green Fluorescent Proteins</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Integrases - genetics</subject><subject>Isoenzymes - genetics</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>PERF15</subject><subject>Phosphoglycerate Kinase - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Spermatogenesis - physiology</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>testis</subject><subject>Transgenes - genetics</subject><issn>0012-1592</issn><issn>1440-169X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1LwzAUhoMoOqd_QXLlXetJk_QDvBGdUxAUUfAupOnpzGw7TTrc_r2pm3ipuUkgz3tyTh5CKIOYhXU2j5kQELG0WMUJgIgBskzGqx0y-rl42SUjAJZETBbJATn0fg6BFCzZJwdMZgJkzkfk7ekVaY--t2bZaEdr3fdrqo2taGm7ynYz-u4WPdqOPkwer5mkDmeBDBHah2jgkS5qOkPXUoNN4-kv2jvd-Rl21tDWGjwie7VuPB5v9zF5vp48Xd5Ed_fT28uLu8iIMEVkTK1RcJ4yloKsJOOlLLQuMcuSPE8wr6BKq6JmMslSAZU0BdcCsMwTwUXB-ZicbuqGzj-WYTbVWj_0pjtcLL1KM5EzSMWfICtykCKFAOYb0LiF9w5r9e5sq91aMVCDETVXw8erwYgajKhvI2oVoifbN5Zli9VvcKsgAOcb4NM2uP53YXU1vQoH_gXz35iw</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Kido, Tatsuo</creator><creator>Arata, Satoru</creator><creator>Suzuki, Ryusuke</creator><creator>Hosono, Tomohiko</creator><creator>Nakanishi, Yoshinobu</creator><creator>Miyazaki, Jun‐ichi</creator><creator>Saito, Izumu</creator><creator>Kuroki, Toshio</creator><creator>Shioda, Seiji</creator><general>Blackwell Science Pty</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice</title><author>Kido, Tatsuo ; Arata, Satoru ; Suzuki, Ryusuke ; Hosono, Tomohiko ; Nakanishi, Yoshinobu ; Miyazaki, Jun‐ichi ; Saito, Izumu ; Kuroki, Toshio ; Shioda, Seiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4775-ccfae433611605d513b59aabe772882e8d0d6d9f1527640d5c93a40eb82434933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Apoptosis - physiology</topic><topic>Carrier Proteins - metabolism</topic><topic>Cre‐LoxP system</topic><topic>DNA Primers</topic><topic>fatty acid binding protein</topic><topic>Fatty Acid-Binding Proteins</topic><topic>Fertility - genetics</topic><topic>germ cell death</topic><topic>Green Fluorescent Proteins</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Integrases - genetics</topic><topic>Isoenzymes - genetics</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>PERF15</topic><topic>Phosphoglycerate Kinase - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Spermatogenesis - physiology</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>testis</topic><topic>Transgenes - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kido, Tatsuo</creatorcontrib><creatorcontrib>Arata, Satoru</creatorcontrib><creatorcontrib>Suzuki, Ryusuke</creatorcontrib><creatorcontrib>Hosono, Tomohiko</creatorcontrib><creatorcontrib>Nakanishi, Yoshinobu</creatorcontrib><creatorcontrib>Miyazaki, Jun‐ichi</creatorcontrib><creatorcontrib>Saito, Izumu</creatorcontrib><creatorcontrib>Kuroki, Toshio</creatorcontrib><creatorcontrib>Shioda, Seiji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development, growth & differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kido, Tatsuo</au><au>Arata, Satoru</au><au>Suzuki, Ryusuke</au><au>Hosono, Tomohiko</au><au>Nakanishi, Yoshinobu</au><au>Miyazaki, Jun‐ichi</au><au>Saito, Izumu</au><au>Kuroki, Toshio</au><au>Shioda, Seiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice</atitle><jtitle>Development, growth & differentiation</jtitle><addtitle>Dev Growth Differ</addtitle><date>2005-01</date><risdate>2005</risdate><volume>47</volume><issue>1</issue><spage>15</spage><epage>24</epage><pages>15-24</pages><issn>0012-1592</issn><eissn>1440-169X</eissn><abstract>The quality control of sperm is critical for efficient reproduction. In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is related to fertility. Composition of the fatty acid of germ cells changes dynamically during spermatogenesis, and fatty acid binding protein (FABP) may be involved in these changes. In this study, we developed transgenic mice with a testicular germ‐cell‐specific FABP (PERF15) transgene, whose expression was controlled by the Cre‐LoxP site‐specific recombination system. We also developed transgenic mice with the Cre gene under the control of the spermatocyte specific Pgk2 promoter. In double transgenic mice, following Cre‐mediated recombination of the PERF15 containing transgene, PERF15 was strongly overexpressed. Its overexpression induced multinucleate symplasts to form, indicating programmed germ cell death occurred at the elongated spermatid stage. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; Freely Accessible Japanese Titles; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Apoptosis - physiology Carrier Proteins - metabolism Cre‐LoxP system DNA Primers fatty acid binding protein Fatty Acid-Binding Proteins Fertility - genetics germ cell death Green Fluorescent Proteins Immunoblotting Immunohistochemistry Integrases - genetics Isoenzymes - genetics Male Mice Mice, Transgenic PERF15 Phosphoglycerate Kinase - genetics Promoter Regions, Genetic - genetics Reverse Transcriptase Polymerase Chain Reaction Spermatogenesis - physiology Spermatozoa - metabolism Spermatozoa - physiology testis Transgenes - genetics |
| title | The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice |
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